pGEX/GST gene fusion problems-> HELP!

[Michael DiDonato]

In article <32227081.3C92 at po.cwru.edu>, sbc4 at po.cwru.edu wrote:

> I am trying to find if there is a limit on the size of a GST-fusion
> protein. My protein is 110Kd + GST (27kd) for a total of 137kd.
> 
> Any thoughts on this?
> 
> Steve

I am expressing a 96Kda GST fusion no problems.  However I have heard that
the higher you go the less binds to the column because of stearic
constrains.

Good Luck.
Mike