Thrombin Cleavage of GST

AShaw5 ashaw5 at
Tue Aug 27 17:31:27 EST 1996

We usually immunize with the fusion protein.  While we are waiting for the
first boost, we prepare the cleaved product and then boost with that. 
That should focus the immune response onto the protein of interest.  When
we screen we always do our ELISAs on both GST, the GST fusion and the
cleaved product.

Andrey Shaw

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