pGex expression

AShaw5 ashaw5 at aol.com
Tue Aug 27 17:31:35 EST 1996


We usually just sonicate in PBS alone.  Having detergent in the buffer is
going to cause a lot of foaming.  After sonicating , we then add triton or
np40 to 1%, then spin out the insoluble debris.
Andrey Shaw



More information about the Methods mailing list