Glycogen for co-precipitating DNA

Edmundo Castro ecastro at physci.ucla.edu
Tue Aug 27 18:27:51 EST 1996


Frederik Intelmann wrote:
> 
> In article <jwobrien-2008961033410001 at f181-203.net.wisc.edu>,
>    jwobrien at students.wisc.edu (jwobrien) wrote:
> >Does anyone have references for co-precipitating DNA with glycogen?  Any
> >protocols?  i.e. what concnetration is best.
> 
> From the Boehringer instruction (I got a copy, but haven't used the stuff
> myself yet):
> 
> - "20 µg glycogen = approx 1 µl solution allow to precipitate pg-amounts of
>    DNA or RNA from a volume of 1 ml."
> 
> - literature: 1. Tracy, S. (1981) Prep. Biochem. 11, 251-268
>               2. Helms, C. et al (1985) DNA 4, 39-49
> 
> Hope this helps
>            Frederik


This is what I have used in the past and it works very well.

EC



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