standard for northern blot analysis

Dr. Benoit Gauthier bgauthie at OHI-NET.HEARTINST.ON.CA
Tue Aug 27 13:16:34 EST 1996


Problem; The mRNAs which I have been using to standardize my northen
blots (GAPDH and Actin) are both modulated in response to different
treatment that I have been giving my cells.

Question: Are their any other internal controls that I can use in order
to standardize my mRNA of interest which are refractory to most
treatment and which are found in adipose tissues. I cannot use ribosomal
RNA since I isolate poly(A) RNA.

			Thank you



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