standard for northern blot analysis
Dr. Benoit Gauthier
bgauthie at OHI-NET.HEARTINST.ON.CA
Tue Aug 27 13:16:34 EST 1996
Problem; The mRNAs which I have been using to standardize my northen
blots (GAPDH and Actin) are both modulated in response to different
treatment that I have been giving my cells.
Question: Are their any other internal controls that I can use in order
to standardize my mRNA of interest which are refractory to most
treatment and which are found in adipose tissues. I cannot use ribosomal
RNA since I isolate poly(A) RNA.
More information about the Methods