Plasmid amplification in Cm-r E.coli

DAVE BOYD dboyd at cc.umanitoba.ca
Tue Aug 27 10:57:03 EST 1996


In article <321CD884.5123 at rtc.riken.go.jp>, Masayoshi Itoh
<maitoh at rtc.riken.go.jp> wrote:

> Hello!
> Can anyone tell me how to amplify plasmid in Cm-resistant E. coli?
> Of course I know changing the host is a better way.
> But I cannot do it.  I want to prepare enough amount plasmids from a
> less E.coli cells.
> Thanks in advance for your help.
> 
> Masayoshi Itoh
> maitoh at rtc.riken.go.jp

Use spectinomycin at 35 ug/ml

DAB

-- 
Hey, hey, hey, hey! It was the DNA.
Hey, hey, hey, hey! That made me this way.



More information about the Methods mailing list