Plasmid amplification in Cm-r E.coli

DAVE BOYD dboyd at
Tue Aug 27 10:57:03 EST 1996

In article <321CD884.5123 at>, Masayoshi Itoh
<maitoh at> wrote:

> Hello!
> Can anyone tell me how to amplify plasmid in Cm-resistant E. coli?
> Of course I know changing the host is a better way.
> But I cannot do it.  I want to prepare enough amount plasmids from a
> less E.coli cells.
> Thanks in advance for your help.
> Masayoshi Itoh
> maitoh at

Use spectinomycin at 35 ug/ml


Hey, hey, hey, hey! It was the DNA.
Hey, hey, hey, hey! That made me this way.

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