Qiagen: This is ridiculous

[sharmaa at acsu.buffalo.edu]

I havent expereinecsIan A. York wrote:
> 
> I've been using Qiagen DNA preps for years.  The quality is great; as
> everyone who's used them knows, though, the yield is at best
> inconsistent.  At random intervals, and for no apparent reason, there
> will be absolutely no DNA produced.  I've been willing to deal with that
> when I was getting 5 out of 6, or whatever, with good yields; with
> important DNA, I'd always run duplicates to be reasonably sure of getting
> something.
> 
> In the last two days, I've run 10 Qiagen DNA preps - 8 midi-preps, two
> maxipreps - and got one (1) with detectable DNA.  Meanwhile two identical
> cultures, run on a different company's resin, gave 160 ug and 175 ug of
> DNA from 200 ml bugs (low copy-number plasmid).
> 
> One out of ten is not acceptable.  Over the years I've gone around and
> around with Qiagen reps, I've tried dozens of variations on the protocol,
> I've tried everything they suggest, I follow the directions exactly, and
> in spite of it I just spent two days pouring DNA down the sink.
> 
> The hell with it.  Until I hear from users that the Qiagen preps are
> reliable, I'm not going to use them any more.
> 
> Blowing off steam,
> 
> Ian

I have had low or no yields with  Qia-miniprep spin columns! I will be 
doing a comparison of Qiagen midiprep with PrimmLabs kit soon. Will 
post the results.
  
- 
Name: Ashu Sharma                       
E-mail: sharmaa at acsu.buffalo.edu