CAT gene -- fluorescent substrates?
ebeaty at firecraker.com
Tue Aug 27 10:46:33 EST 1996
Check out Molecular Probes. They have a fluorescent CAT assay, and may
also have or know of a CAT substrate with the properties you're looking
for. Their tech assistance line is: (541) 465-8353.
Pierce Chemical Company
Gene Huh wrote:
> Hello fellow netters,
> I have a question for those who work with the chloramphenicol
> acetyltransferase gene. I was wondering if a substrate for CAT exists
> (i) is fluorescent and
> (ii) has a reaction product that is retained within a live cell.
> Such a substrate night work in the same way that FDG
> (fluorescein-D-galactopyranoside) works for live staining of LacZ-positive
> Thanks in advance,
> Dr. Gene S. Huh
> Dept. of Molecular and Cell Biology
> University of California at Berkeley
> gshuh at garnet.berkeley.edu
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