DNA contanimate

Joe Boutell boutell at cf.ac.uk
Tue Aug 27 06:54:48 EST 1996


Gabby Gerlitz <gabiger at zoot.tau.ac.il> wrote:
>Hello everyone,
>
>I got DNA in my pipetors, and it contanimate my ligation reactions.
>Does anyone know what is the best way to clean pipetors from DNA?
>I have tried to clean them with Radio wash solution without much success.
>
>Thanks,
>Gabi.
>E-mail: gabiger at post.tau.ac.il

Around here we take the pipettes apart, soak the barrels in 1M HCl 
overnight and the O-rings in sterile water. In the morning, give 
everything a good rinse with water and reassemble.
Seems to work for us.
Cheers,
Joe Boutell. 




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