looking for a method to strip gel plates

H. ROYCHOWDHURY hroychow at NMSU.EDU
Wed Aug 28 10:03:12 EST 1996


On 27 Aug 1996, brad swanson wrote:

> Hi, 
> 	I use a BioRad sequencing gel apparatus to run out microsatellite DNA and
> I coat one of the plates with Sigmacote to make it 'slick'.  I have noticed
> recently that the gels are starting to have difficultly staying on one
> plate.  Someone suggested that I need to strip the plates of the Sigmacote
> b/c the plates become 'overcoated' over time.  Does anyone have a recipe
> for a stripping reagent?  Thanks in advance!  Cathy Mossman
> 
> 

Soak the plate(s) in 0.1N NaOH overnight.
			>>>>>>>>>>>>>>>>>>>>>>>>>>>
			  Dr. Hiranya S. Roychowdhury
   			  Plant Genetic Engineering Lab.
			  Box 3GL, NM State Univ.
			  Las Cruces, NM 88003
			  Phone: (505) 646-5785
			  hroychow at nmsu.edu
			<<<<<<<<<<<<<<<<<<<<<<<<<<<




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