looking for a method to strip gel plates

Wed Aug 28 10:03:12 EST 1996

On 27 Aug 1996, brad swanson wrote:

> Hi, 
> 	I use a BioRad sequencing gel apparatus to run out microsatellite DNA and
> I coat one of the plates with Sigmacote to make it 'slick'.  I have noticed
> recently that the gels are starting to have difficultly staying on one
> plate.  Someone suggested that I need to strip the plates of the Sigmacote
> b/c the plates become 'overcoated' over time.  Does anyone have a recipe
> for a stripping reagent?  Thanks in advance!  Cathy Mossman

Soak the plate(s) in 0.1N NaOH overnight.
			  Dr. Hiranya S. Roychowdhury
   			  Plant Genetic Engineering Lab.
			  Box 3GL, NM State Univ.
			  Las Cruces, NM 88003
			  Phone: (505) 646-5785
			  hroychow at nmsu.edu

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