Looking for different CAT assay protocols !

Richard A. Janssen rjanssen_imm at OPAL.TUFTS.EDU
Wed Aug 28 21:10:47 EST 1996


Hi,

Boehringer-Mannheim has an ELISA kit for quantifying CAT.

Richard
New England Medical Center
Hematology/Oncology

On 28 Aug 1996, Phillip Thompson wrote:

> Thomas,
> 
> I don't know of a review on CAT asaay protocols, but if you surf to
> Promega's WWW site (www.promega.com) & download their tech document on
> CAT assays (cat # E1000) you will have a comparison of two different
> detection methods.  The first outlines detection by extracting the
> assay sample with mixed xylenes.  The butyrylated chloramphenicol
> partitions into the solvent phase, the un-butyrylated into the aqueous
> phase.  Promega claim it is more sensitive than detection by TLC; it
> certainly is quicker & easier to perform!
> 
> 
> 
> ***************************************
> Phillip R. Thompson, PhD
> Senior Research Officer
> HIV Molecular Biology Unit
> Macfarlane Burnet Centre
> Yarra Bend Rd
> Fairfield, Vic 3078
> Australia
> Phone: (03) 9282 2127
> Fax: (03) 9282 2100
> ***************************************
> 
> 



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