Does Taq Stoffel fragment produce 3' A overhangs.
nourse at tpp.uq.edu.au
Thu Aug 29 04:59:22 EST 1996
We've been using Perkin-Elmer AmpliTaq Stoffel fragment for DNA
fingerprinting and would like to clone some of the pcr products. We've
been having troubles using Promega's pGEM-T kit and were just
wondering if anyone knows if this enzyme produces 3' A overhangs as
taq polymerase does. Also, what do people think of the pGEM-T kit, we
seem to have got a few duds from them. Thanks alot.
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