Looking for different CAT assay protocols !

Phillip Thompson thompsop at burnet.mbcmr.unimelb.edu.au
Wed Aug 28 18:58:01 EST 1996


I don't know of a review on CAT asaay protocols, but if you surf to
Promega's WWW site (www.promega.com) & download their tech document on
CAT assays (cat # E1000) you will have a comparison of two different
detection methods.  The first outlines detection by extracting the
assay sample with mixed xylenes.  The butyrylated chloramphenicol
partitions into the solvent phase, the un-butyrylated into the aqueous
phase.  Promega claim it is more sensitive than detection by TLC; it
certainly is quicker & easier to perform!

Phillip R. Thompson, PhD
Senior Research Officer
HIV Molecular Biology Unit
Macfarlane Burnet Centre
Yarra Bend Rd
Fairfield, Vic 3078
Phone: (03) 9282 2127
Fax: (03) 9282 2100

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