How to denature target DNA on nylon membrane?

CRH nil
Wed Aug 28 09:42:48 EST 1996

> Hi netters
> Does anyone know what methods be able to use to denature DNA on nylon
> directly? Thanks
> Jeremy

Hi Jermy,
Recall the screening of the plasmid or phage library. Do you guess
anything from the protocol you use?
there we just pick up the colonies/plaques onto the nylon membrane and fix
and denature the DNA onto membrane...I would give you this explanation to
your question..hope it sounds logical and reasonable
Good Luck
4104vsav at

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