How to denature target DNA on nylon membrane?

CRH nil
Wed Aug 28 09:42:48 EST 1996


> Hi netters
> Does anyone know what methods be able to use to denature DNA on nylon
membrane 
> directly? Thanks
> Jeremy

Hi Jermy,
Recall the screening of the plasmid or phage library. Do you guess
anything from the protocol you use?
there we just pick up the colonies/plaques onto the nylon membrane and fix
and denature the DNA onto membrane...I would give you this explanation to
your question..hope it sounds logical and reasonable
Good Luck
Savita
4104vsav at vm1.ulaval.ca



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