Other minipreps (was Re: Qiagen: This is ridiculous)

[Robert Seymour]

In article <50254c$1lqs at piglet.cc.uic.edu>,
   levenson at uic.edu (Victor Levenson) wrote:
>sharmaa at acsu.buffalo.edu wrote:
>
>>I havent expereinecsIan A. York wrote:
>>> 
>>> I've been using Qiagen DNA preps for years.  The quality 
is great; as
>>> everyone who's used them knows, though, the yield is at 
best
>>> inconsistent.  At random intervals, and for no apparent 
reason, there
>[stuff deleted]
>>> Blowing off steam,
>>> 
>>> Ian
>
>>I have had low or no yields with  Qia-miniprep spin 
columns! I will be 
>>doing a comparison of Qiagen midiprep with PrimmLabs kit 
soon. Will 
>>post the results.
>>  
>>Name: Ashu Sharma                       
>>E-mail: sharmaa at acsu.buffalo.edu
>
>On the same subject:
>
>Has anyone tried mimipreps from "5'-3'" which they call
>"Perfect-Prep"? And/Or  from Princeton Separations (forgot 
the name
>for this one)? The tech people for both companies claim 
better than
>CsCL ("and other kits" - no names, though) purity and 
yield, but what
>is your experience? The applications I am mostly interested 
in are
>TRANSFECTIONS and AUTO Sequencing (for screening by RE 
digest nothing
>beats phenol-chloroform ;-)!)
>
>If you want  to e-mail I can summarize and post the gist of 
the
>responses.
>
>Cheers,
>
>Victor
>levenson at uic.edu
>

Dear All

As we are discussing miniprep protocols I thought I might 
mention the protocol that I've just seen in biotechniques. 
It is an alkaline lysis followed by a PEG 6000 precipitation 
of the DNA followed by a LiCl precipitation of RNA and 
protein away from the DNA.  The authors claim that this 
gives sequenceable DNA.  I have not tried this method yet 
but will probably do so in the next week or so.  The 
reference is BioTechniques Vol 21 pages 233-235.

Robert