3' prime radiolabeling of oligo using dideoxynucleotides

p.j. van santbrink santbrin at CHEM.LEIDENUNIV.NL
Wed Aug 28 06:52:07 EST 1996


Fellow netters,     

I am facing some problems with radiolabeling of a oligodeoxynucleotide 
since the 5'-prime site is already chemically derivatized. Im am now 
planning to chemically radiolabel the ODN at the 3'-prime. However, the 
stability of ODNs radiolabeled at the 3'-prime using 32P-ATP is low. Does 
anyone know whether the stability of 3'-dideoxy labeled ODN is enhanced 
as compared to deoxylabeled ODNs. Thank you in advance. 

 
Best regards to lovely superpam. 
-- 
Leiden Institute of Chemistry, Gorlaeus Laboratories.
Leiden University;Einsteinweg 55 / P.O. Box 9502 2300RA, Leiden The 
Netherlands.
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