3' prime radiolabeling of oligo using dideoxynucleotides

[p.j. van santbrink]

Fellow netters,     

I am facing some problems with radiolabeling of a oligodeoxynucleotide 
since the 5'-prime site is already chemically derivatized. Im am now 
planning to chemically radiolabel the ODN at the 3'-prime. However, the 
stability of ODNs radiolabeled at the 3'-prime using 32P-ATP is low. Does 
anyone know whether the stability of 3'-dideoxy labeled ODN is enhanced 
as compared to deoxylabeled ODNs. Thank you in advance. 

 
Best regards to lovely superpam. 
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