3' prime radiolabeling of oligo using dideoxynucleotides
p.j. van santbrink
santbrin at CHEM.LEIDENUNIV.NL
Wed Aug 28 06:52:07 EST 1996
I am facing some problems with radiolabeling of a oligodeoxynucleotide
since the 5'-prime site is already chemically derivatized. Im am now
planning to chemically radiolabel the ODN at the 3'-prime. However, the
stability of ODNs radiolabeled at the 3'-prime using 32P-ATP is low. Does
anyone know whether the stability of 3'-dideoxy labeled ODN is enhanced
as compared to deoxylabeled ODNs. Thank you in advance.
Best regards to lovely superpam.
Leiden Institute of Chemistry, Gorlaeus Laboratories.
Leiden University;Einsteinweg 55 / P.O. Box 9502 2300RA, Leiden The
Phone: +31 71 5274400 TeleFax: +31 71 5274537
Department: Phone: +31 71 527
More information about the Methods