Yeast contamination on cell culture:HELP!!

[Joao Vasconcelos Costa]

Xose S. Puente wrote:
> 
> Could somebody helps me???
> I`m working on culture of human cell lines and I have some troubles
> with yeast contamination. Somebody knows how I can discart them in my
> cultures? I`ve tried with Nystatin and excess of antibiotic, but It
> doesn`t work.
> Please, I need Help!!!

Are your cultures irreplaceable? If not, my suggestion is that you
immediately reject those cultures, otherwise you risk to spread the
contamination to all your cell lines. I tried once to cure some yeast-
infected cells and that was happened.

Joao

-- 
*****   JOAO VASCONCELOS COSTA, MD, PhD
*****   Instituto Gulbenkian de Ciencia - Oeiras, Portugal
*****   mailto:jcosta at pen.gulbenkian.pt
*****   http://www.pen.gulbenkian.pt/v2/jvc.html