DNA size markers

[Dr. Duncan Clark]

In article <32221290.3700 at uottawa.ca>, "\"Dr. Douglas A. Johnson\""
<johnson at uottawa.ca> writes
>We have been using purchased phiX174 digested with HaeIII as the low 
>molecular weight size marker for our RAPD gels. The cost is mounting as 
>we turn more and more to RAPD technology. Can anyone suggest an easily 
>made DNA/restriction enzyme combination that could substitute for the 
>commercial marker set? I would prefer a sequenced plasmid as plasmid 
>isolation in our hands results in much higher yields that phage RF.
>
>Thanks
>
>Doug Johnson
>
>djohnson at oreo.uottawa.ca


pUC18/Sau3AI or pBR322/Hae
-----------------------------------------------------------------------------
My mind's made up. Don't confuse me with the facts!
Duncan Clark
GeneSys Ltd.
http://www.dnamp.com