DNA size markers

Dr. Duncan Clark duncan at genesys.demon.co.uk
Thu Aug 29 05:03:40 EST 1996

In article <32221290.3700 at uottawa.ca>, "\"Dr. Douglas A. Johnson\""
<johnson at uottawa.ca> writes
>We have been using purchased phiX174 digested with HaeIII as the low 
>molecular weight size marker for our RAPD gels. The cost is mounting as 
>we turn more and more to RAPD technology. Can anyone suggest an easily 
>made DNA/restriction enzyme combination that could substitute for the 
>commercial marker set? I would prefer a sequenced plasmid as plasmid 
>isolation in our hands results in much higher yields that phage RF.
>Doug Johnson
>djohnson at oreo.uottawa.ca

pUC18/Sau3AI or pBR322/Hae
My mind's made up. Don't confuse me with the facts!
Duncan Clark
GeneSys Ltd.

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