Looking for different CAT assay protocols !

Keith Leppard Keith.Leppard at warwick.ac.uk
Thu Aug 29 07:06:58 EST 1996


th.neumann at dkfz-heidelberg.de wrote:
>
> Hello,
> 
> does anyone know a good review on the different types of CAT assay 
> protocols and their sensitivity ?
> 
> Thanks for help,
> 
> thomas 
> 
One big plus for the CAT assay method which involves partition of 
labelled product into scintillant (Neumann et al BioTechniques 5, 444
-447 (1987); Eastman ibid 730-732) is that you can take data from a 
reaction over a time course and hence establish the initial rate of 
conversion of substrate. Only this is a true reflection of the amount
of CAT enzyme present, and since there are enough other assumptions
involved in using an amount of enzyme as a measure of the transcrip
tional activity of the promoter driving its synthesis, it is good to
get the amount of enzyme right.
I hope this is useful.
Keith



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