lower detection limit with Coomssie?

Dima Klenchin klenchin at facstaff.wisc.edu
Thu Aug 29 16:10:15 EST 1996


In article <322614a2.275284 at usenet.ufl.edu>,
   bwr at icbr.ifas.ufl.edu (Bruce W. Ritchings) wrote:
->Hello Folks,
->	For DNA work, the limit of visibility of a DNA band in an
->agarose gel is known to be around 10-15 ng.There are lots of
->variables, but that's the figure that's usually given (see Messing's
->primer on molecular biology, some years back). Is there a standard
->figure given for the limits of visibility for Coomassie-stained
->proteins in an SDS-PAGE gel? Any help greatly appreciated, thanks,
->Bruce Ritchings, University of Florida

Typically 100 ng is given in books. In my experience, on minigels 
stained with Coomasie G-250, ~ 20 ng is possible. 

- Dima





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