postivie control of HIV-1 Amplicor

Alex Chang lpss at unixg.ubc.ca
Thu Aug 29 23:07:29 EST 1996


Out of curiosity, I did an electrophoresis of the positive control (from 
Amplicor HIV-1 Amplification Kit) PCR amplified using unlabelled primers, 
SK462 and SK431.

To my surprise, the amplified product is only around 100 bp. In my 
impression, the positive control product should be the same length (142 
bp) with the target, HIV-1 gag.

Anyone has information about this?

I'd also like to know whether the positive control is an insert in a 
plasmid. Any reference to the construction? 

Thanks

Alex Chang
Pathology
University of British Columbia
achang at hivnet.ubc.ca



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