postivie control of HIV-1 Amplicor
lpss at unixg.ubc.ca
Thu Aug 29 23:07:29 EST 1996
Out of curiosity, I did an electrophoresis of the positive control (from
Amplicor HIV-1 Amplification Kit) PCR amplified using unlabelled primers,
SK462 and SK431.
To my surprise, the amplified product is only around 100 bp. In my
impression, the positive control product should be the same length (142
bp) with the target, HIV-1 gag.
Anyone has information about this?
I'd also like to know whether the positive control is an insert in a
plasmid. Any reference to the construction?
University of British Columbia
achang at hivnet.ubc.ca
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