NH4Ac-Miniprep becomes bad - why?

[Jose E. Mejia]

> Just a guess... Do you store your Amm.Acetate soution at room 
> Temperature? In that case, you do not have much of it left in soln. after 
> a month.

1. What's happening to the ammonium acetate solution stored at room
temperature? Is there a known explanation?

2. The lysis solution is unstable, because atmospheric CO2 dissolves into
the alkaline solution very readily and neutralizes NaOH. A fine
precipitate of sodium carbonate may be visible.

Jose

-- 
Wellcome Trust Centre for Human Genetics
Windmill Road, Oxford OX3 7BN, UK
Tel: +44 1865 740021
Fax: +44 1865 742186
Email: jose.mejia at well.ox.ac.uk