NH4Ac-Miniprep becomes bad - why?
Jose E. Mejia
jose.mejia at well.ox.ac.uk
Thu Aug 29 12:39:05 EST 1996
> Just a guess... Do you store your Amm.Acetate soution at room
> Temperature? In that case, you do not have much of it left in soln. after
> a month.
1. What's happening to the ammonium acetate solution stored at room
temperature? Is there a known explanation?
2. The lysis solution is unstable, because atmospheric CO2 dissolves into
the alkaline solution very readily and neutralizes NaOH. A fine
precipitate of sodium carbonate may be visible.
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