lower detection limit with Coomssie?

Bruce W. Ritchings bwr at icbr.ifas.ufl.edu
Thu Aug 29 17:16:56 EST 1996


Hello Folks,
	For DNA work, the limit of visibility of a DNA band in an
agarose gel is known to be around 10-15 ng.There are lots of
variables, but that's the figure that's usually given (see Messing's
primer on molecular biology, some years back). Is there a standard
figure given for the limits of visibility for Coomassie-stained
proteins in an SDS-PAGE gel? Any help greatly appreciated, thanks,
Bruce Ritchings, University of Florida



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