a good protocol for far westerns/overlays??

Gerald W. Hart gwhart at bmg.bhs.uab.edu
Fri Aug 30 10:14:42 EST 1996

hey all,
         does anybody have or know where i can get a good (tried and
tested) protocol for doing far westerns (protein overlays)??  so far, i've
attempted doing it using a standard western blot protocol and i end up
with very dirty looking exposures of the blots (i've already eliminated
adjusting exposure time by ecl, washing a gazillion times and decreasing
the concentration of the secondary antobody...).  any help would be
appreciated...thx much...please email to siyer at bmg.bhs.uab.edu .

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