Remove SDS from protein preparation

W R BENNETT bspwrb at bath.ac.uk
Fri Aug 30 10:30:48 EST 1996


In the referenced article, wumolbio at mail.ncku.edu.tw writes:
>Dear Netter,
>
>I would grateful if anyone out-there could give any trick to remove SDS
>from protein preparation.  SDS interfer our protein assay by Bio-Rad
>reagent for Braford methods which is the only choice for my protein due
>to lack of those amino acids contributes to the absorption of Lowry
>methods.  
>
Pierce and Warriner sell a detergent-tolerant protein assay reagent which 
is considerably more expensive than the Coomassie-based (Bio-Rad style) 
reagent but tolerates 1% SDS.

Bill Bennett



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