Selection with puromycin

Ashok Aiyar aiyar at ebv.oncology.wisc.edu
Fri Aug 30 15:12:54 EST 1996


On 30 Aug 1996 18:41:26 GMT, Michael W. Thompson <mthom0 at pop.uky.edu> wrote:
>     Out of curiosity, does anyone know the mechanism behind the puromycin resis
>´¾@´¾@P
>marker (i.e., is it a transporter that removes puromycin from the cell?)

I do not think it is a transporter.  The protein is very small 
(~ 200 a.a), and I think it is an acetyl transferase that inactivates 
the drug by acetylating it.

Ashok
-- 
Ashok Aiyar, Ph.D.                      
Department of Oncology            email: aiyar at ebv.oncology.wisc.edu
University of Wisconsin-Madison                  tel: (608) 262-6697




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