Selection with puromycin
aiyar at ebv.oncology.wisc.edu
Fri Aug 30 15:12:54 EST 1996
On 30 Aug 1996 18:41:26 GMT, Michael W. Thompson <mthom0 at pop.uky.edu> wrote:
> Out of curiosity, does anyone know the mechanism behind the puromycin resis
>marker (i.e., is it a transporter that removes puromycin from the cell?)
I do not think it is a transporter. The protein is very small
(~ 200 a.a), and I think it is an acetyl transferase that inactivates
the drug by acetylating it.
Ashok Aiyar, Ph.D.
Department of Oncology email: aiyar at ebv.oncology.wisc.edu
University of Wisconsin-Madison tel: (608) 262-6697
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