cloning in M13

Tony Raizis tonyr at chch.planet.org.nz
Fri Aug 30 06:11:33 EST 1996


Has anyone out there had experience with cloning large fragments i.e.
greater than 1kb in M13mp18? The literature supposedly states that it's
no problem if you use a competent cell strain which is recA- e.g. JM109.
I would feel more comfortable if someone with hands on experience could
verify this and perhaps give us a few useful hints on the matter.

Thanks
Tony



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