Qiagen: This is ridiculous

Oliver S. DePeyer saspeyer at reading.ac.uk
Fri Aug 30 04:37:38 EST 1996


> I have never had a problem, one question, what is your bacterial strain? 
> Also do not overload the column.

Answers:
strain is DH5-alpha, overnight growth in LB
As to overloading the column... actually, we found that we IMPROVED the 
yield from the column if we went above the suggested amount of culture. 
The protocol says 3ml, but we found we got more plasmid if we used 9 or 10ml.



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