Selection with puromycin

[Danny Altschuler]

Hey, I need to do a selection with Puromycin. Vector (pBabePuro - 
infection), cells HT29 colon carcinoma.). I know that I have to do a 
curve but could anyone give a good concentration range?
I have experience with Neo and Hygro and in my hands a good concentr. for 
these drugs is ~100-200ug/ml (hygro), and 400-600ug/ml active comp. (neo).
Which will be the magic number for puro??
Thanks, Danny
Please e-mail me to
altschul at med.unc.edu