Cloning PCR products

Gary Kucera kucera~gt at glaxo.com
Mon Aug 19 07:49:24 EST 1996


It is not necessary to kinase the PCR product prior to subcloning if the 
primers have been de-protected following synthesis.

You will, however, want to "polish" the ends by doing an additional 5 
minute extension at the end of your PCR program to ensure that all 
fragments are full length.  It is even better to incorporate unique 
restriction sites into your primers so that a digest following PCR will 
produce nice ends for subcloning.

________________________________________________________
Gary T. Kucera, Scientist
Cellular Sciences/Transgenics Group
GlaxoWellcome Research, Inc.
Five Moore Drive
Research Triangle Park, NC 27709





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