Cloning PCR products
kucera~gt at glaxo.com
Mon Aug 19 07:49:24 EST 1996
It is not necessary to kinase the PCR product prior to subcloning if the
primers have been de-protected following synthesis.
You will, however, want to "polish" the ends by doing an additional 5
minute extension at the end of your PCR program to ensure that all
fragments are full length. It is even better to incorporate unique
restriction sites into your primers so that a digest following PCR will
produce nice ends for subcloning.
Gary T. Kucera, Scientist
Cellular Sciences/Transgenics Group
GlaxoWellcome Research, Inc.
Five Moore Drive
Research Triangle Park, NC 27709
More information about the Methods