a good protocol for far westerns/overlays??

Nikolai Chitaev nikolaic at VISAR.WUSTL.EDU
Fri Aug 30 18:17:16 EST 1996

> From: gwhart at bmg.bhs.uab.edu (Gerald W. Hart)
> Subject: a good protocol for far westerns/overlays??
> Date: Fri, 30 Aug 1996 16:14:42 +0100
> hey all,
> does anybody have or know where i can get a good (tried and
> tested) protocol for doing far westerns (protein overlays)?? so far,
> i've
> attempted doing it using a standard western blot protocol and i end up
> with very dirty looking exposures of the blots (i've already
> eliminated
> adjusting exposure time by ecl, washing a gazillion times and
> decreasing
> the concentration of the secondary antobody...). any help would be
> appreciated...thx much...please email to siyer at bmg.bhs.uab.edu .

The protocol given in my article (J. Cell Biol. 1996. 133:359-369) I
used with fair results. AP-detection system.

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