kucera~gt at glaxo.com
Tue Aug 20 13:13:30 EST 1996
M.MECRIN <100616.2253 at CompuServe.COM> wrote:
>WE HAVE PROBLEM OF INHIBITION IN RT-PCR.
>DO YOU KNOW WHICH INHIBITOR IS ABLE TO PREVENT THE TAG POLYMERASE
>TO BE EFFECTIVE ?
>WHAT CAN WE DO TO SUPPRESS IT ?
>THANK YOU FOR SOME IDEAS ...
You need to include a little more info here. Where are you having the
problem, during first-strand synthesis or your PCR? Are you sure you
have an inhibitor problem or could your RNA be bad? Have you looked at
the RNA on a gel?
Gary T. Kucera, Scientist
Cellular Sciences/Transgenics Group
GlaxoWellcome Research, Inc.
Five Moore Drive
Research Triangle Park, NC 27709
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