RT-PCR

[Gary Kucera]

M.MECRIN <100616.2253 at CompuServe.COM> wrote:
>WE HAVE PROBLEM OF INHIBITION IN RT-PCR.
>
>DO YOU KNOW WHICH INHIBITOR IS ABLE TO PREVENT THE TAG POLYMERASE 
>TO BE EFFECTIVE ?
>
>WHAT CAN WE DO TO SUPPRESS IT ?
>
>THANK YOU FOR SOME IDEAS ...
>
>-- 
>Michel MECRIN


You need to include a little more info here.  Where are you having the 
problem, during first-strand synthesis or your PCR?  Are you sure you 
have an inhibitor problem or could your RNA be bad?  Have you looked at 
the RNA on a gel?

________________________________________________________
Gary T. Kucera, Scientist
Cellular Sciences/Transgenics Group
GlaxoWellcome Research, Inc.
Five Moore Drive
Research Triangle Park, NC 27709