Ni-chelation and Mg-binding

Troels Wind wind at biobase.dk
Fri Aug 30 08:27:58 EST 1996


Hi all,

I have a question regarding Ni-chromatography of His-tagged proteins.

What if your protein has a Mg-binding site, wont it stick via that 
as well, i.e. wont the Ni ions on the column be able to replace
Mg in the site?

Just wondering...

Cheers,
Troels




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