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Methods August 1996 Archives by author

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Starting: Thu Aug 1 00:34:52 EST 1996
Ending: Sat Aug 31 15:12:12 EST 1996
Messages: 1169

who makes 'OLIGO' software? - forget them! go with DNASTAR! "Stephen A. Bustin 3123", SURG
reply: SSCP: Restriction Enzymes vs. Shorter Fragments Gregory S. Buzard 'Buz'
Reasons for PCR failure....? Gregory S. Buzard 'Buz'
PCR-SSCP loading mixture/denaturant?? Gregory S. Buzard 'Buz'
Removal of Ethidium Bromide Ed Seijo (BIO)
Sequencing PCR product by degenerateprimer *
NH4Ac-Miniprep becomes bad - why? *
Patent on GeneClean? *
Patent on GeneClean? *
NH4Ac-Miniprep: The Protocol *
MICROEXTRACTION.
E. coli host strain for transformation .69g
Reasons for PCR failure....? :-Peter
E. coli host strain for transformation ?=4~$^
RNA PURIFICATION MCCABE AJ
RNA Purification MCCABE AJ
inducible mammalian promoter NETSCAPE APPLICATION
inducible mammalian promoter NETSCAPE APPLICATION
Antibodies to 6-His Tag AShaw5
expressed protein is 5-6kda bigger than AShaw5
Microcentrifuge preferences??? AShaw5
Thrombin Cleavage of GST AShaw5
pGex expression AShaw5
Zoo blots Mr Hassan Abdulrazzak
Second hand zoo blots Mr Hassan Abdulrazzak
dhfr activity assay? Shanthi Adimoolam
BIOSCI/bionet miniFAQ & Fundraiser BIOSCI Administrator
blunt end ligation problems Jeremy C. Ahouse
puromycin selection in e. coli and lymphoblastoid cell lines (LCLs) Ashok Aiyar
Antibodies to 6-His Tag Ashok Aiyar
Cloning PCR products Ashok Aiyar
re-using electroporation cuvettes Ashok Aiyar
Selection with puromycin Ashok Aiyar
Selection with puromycin Ashok Aiyar
pBEND anyone? Ashok Aiyar
Help with DD RT-PCR?? Louis Alarcon
myoglobin cDNA Louis Alarcon
Wanted: Mouse B-cell library Shane Albright
Gene knockout in E.coli P.R. Alefounder
Taq DNA ligase and PCR P.R. Alefounder
Taq data concealed?(was: Re-PCR not allowed) Bill Alexander
Removing endotoxin from plasmids Mikhail Alexeyev
size of pBI 121? Mikhail Alexeyev
Specificity of NEM toward cysteine residues Mikhail Alexeyev
Invitrogen's pZERO & E.coli with ccdA Mikhail Alexeyev
Invitrogen's pZERO & E.coli with ccdA Mikhail Alexeyev
Gene knockout in E.coli Mikhail Alexeyev
Need help with CAT assays in bacteria Mikhail Alexeyev
HELP!!! Lambda prep... Allison
Eliminate light/heavy chain after IP?? Danny Altschuler
Selection with puromycin Danny Altschuler
GST fusions forming dimers - reference, PLEASE Chen Ho An
long PCR primer Chen Ho An
I need a PROTOCOL !!!! Victor Anaya-Baez
Looking for antibodies against Cre-recombinase Thomas R. Anderson
Antibodies to cre recombinase Thomas R. Anderson
anti-heparin and EDTA PCR tricks Dan Andrews
Biotin Pcr primers and anealing temperature Dan Andrews
Help:ss-DNA Anja
NIH Image advice... Dr Alexander J Annala
BioRad CHEF DR-II Pulsed Field Gel Electrophoresis Apparatus Dr. Alexander J. Annala
source of annexin Joanna Aretz
glycerol to avoid protein precipitation?... Arioch
Chloropane ??? what the h... ??? Josee Ash
NH4Ac-Miniprep: The Protocol Josee Ash
E.coli cultures lysing after induction Peter Ashton
Position Open Technical Assistance
FITC fading kinetics Graham Atherton
DNA gel extraction W R BENNETT
Remove SDS from protein preparation W R BENNETT
GST-fusions and proteolysis BIOTEC54
Plasmid amplification in Cm-r E.coli DAVE BOYD
Help-Lg-scale Bacterial Genomic DNA Prep Joel Bader
Carbos. in DNA prep!!!!!! Joel Bader
CAT gene Tim Barnett
DNA Quantitation-Quick and Dirty David Barrass
cloning in M13 Kirk A Bartholomew
cleavage of MBP fusion proteins Guriq Basi
subcloning pcr bands L. Bastian
Reasons for PCR failure....? Jim Beasley
A problem in topology Ed Beaty
DIG-labelled RNA probes Ed Beaty
CAT gene -- fluorescent substrates? Ed Beaty
UV safe TAE ? Ed Beaty
biotinylated nucleotide source? Ed Beaty
DNA-quantification by software Andreas Becker
Primer Design??? Andreas Becker
Taq polymerase Robin Beech
PCR detection of HLA alleles Robin Beech
info on polyclonal antibodies against soybean BBI needed Vadim Beilinson
Re- Smeary Touchdown PCR Brett Beitzel
Re- PCR not allowed Brett Beitzel
Re- Amplitaq Gold Brett Beitzel
Re- Cloning PCR Products Brett Beitzel
Cheap Eporation Cuvettes? Bob Belas
trehalose determination Ed Bellion
Temperature Sensitive pACYC or Derivatives Michael Benedik
freeze-squeeze (Re: geneclean: vortexing) Trevor Bezdek
about transformation of DNA into E.Coli Trevor Bezdek
source for ICE or IchL Trevor Bezdek
Searching desperately for pJM17 (E1 adenovirus) Trevor Bezdek
Taq polymerase patent (not) Tom Bickle
vaccination with HIS tag protein Stephen J Billington
Cosmids Stephen J Billington
BioRad PROTEIN A BINDING BUFFER Jeffrey Bjorge
Harris -80 Freezers Bruce Thomas Blakely
Director Level Position Martin Blankfard
Cloning PCR products Volker Blaschke
Hela Cells and Adenylylcylase Components Tracy Blevins
Gram-positive sequence similarities-info req Maria Bobrowska
DNA electrophoresis Maria Bobrowska
B.napus genomic library Peta C. Bonham-Smith
Poly A Northern normalisation Peta C. Bonham-Smith
Annealing of two complementary circular SS DNAs Joe Boutell
Help me! / Yeast transformation in Two-Hybrid screening Joe Boutell
DNA contanimate Joe Boutell
Chloropane ??? what the h... ??? Robot Boy
Annealing of two complementary circular SS DNAs Chris Boyd
Trizol Susan Boyle-Vavra
Help with PCR library screening John Brennand
Cloning vector with kanamycin instead of ampicillin resistance John Brennand
Size fractionation of DNA John Brennand
How do I terminate transcription at a specific site in a plasmid that transfected into mammalian cells??? Andrew Brooks
HELP HELP HELP needed with RNA extraction Paul A Bucciaglia
How to "normalize" Buddha
Primer Design??? Buddha
Thrombin Cleavage of GST Evan Burkala
Reasons for PCR failure....? Bill Burnett
Oligo-services... Tonja Burshek
RT-PCR difficulty - PLEASE HELP!!!!! Tonja Burshek
RT-PCR difficulty - PLEASE HELP!!!!! Tonja Burshek
catalase/tissue culture Tim Buss
Large MW Standard f. SDSPAGE Dr E. Buxbaum
Fiske and Subbarow reagent . . . Dr E. Buxbaum
Microcentrifuge preferences??? Dr E. Buxbaum
Formaldehyde aliquots Dr E. Buxbaum
Tricks for pipetting detergents?? Dr E. Buxbaum
glycerol to avoid protein precipitation?... Dr E. Buxbaum
Cleaning Quartz Cuvettes Dr E. Buxbaum
test: please, ignore me ! Christine CARTIER
Custom BAC libraries? STACY A CIUFO
geneclean: vortexing Manuel G. CLAROS
HELP HELP HELP needed with RNA extraction Manuel G. CLAROS
How to denature target DNA on nylon membrane? CRH
Help with Genomic PCR Miguel Cabrita
Elongase vs. Expand: Which is better? Miguel Cabrita
ISO: Next Plant Genome Meeting Roy G. Cantrell
Where is the gfp group Roberto Cappai
endotoxin assay kit Leslie Carvalho
VVND Leslie Carvalho
Plasmid pKK3535? Michael Casey
BIOMETRA - Thermoblock ? Michael Casey
Where is the gfp group Warren Casey
Where is the gfp group Warren Casey
Statistics and biology Edmundo Castro
Glycogen for co-precipitating DNA Edmundo Castro
AMBIONS ANTISENSE 18S TEMPLATE Jennifer L Causey
AMBIONS ANTISENSE 18S TEMPLATE Jennifer L Causey
AMBIONS ANTISENSE 18S TEMPLATE Jennifer L Causey
Test - no message Mike Challen
Cy5 Custom DNA Synthesis Dr Alan Chan
Cy5 Custom DNA Synthesis Dr Alan Chan
Cy5 Custom DNA Synthesis Dr Alan Chan
BioRad PROTEIN A BINDING BUFFER Alex Chang
postivie control of HIV-1 Amplicor Alex Chang
How to denature target DNA on nylon membrane? Jeremy Chen
promoter screening Leung Kwan Chi
PCR primer selection software LAU Tze Chin
Carbos. in DNA prep!!!!!! Tim Chipman
Qiagen: This is ridiculous Nikolai Chitaev
Selection with puromycin Nikolai Chitaev
a good protocol for far westerns/overlays?? Nikolai Chitaev
Your basic stupid question about PCR cloning Dr. Duncan Clark
Bind-Silane and sequencing Dr. Duncan Clark
Wedge Sequencing gels Dr. Duncan Clark
DNA size markers Dr. Duncan Clark
Mung bean nuclease ? David Clarke
postivie control of HIV-1 Amplicor Dr. J.P. Clewley
postivie control of HIV-1 Amplicor Dr. J.P. Clewley
pGEX/GST gene fusion problems-> HELP! Steve Cohen
Qiagen refuses to tell! Steven Cohen
Qiagen: This is ridiculous Steven Cohen
test Allan Colthart
RNA Quantification... Allan Colthart
Nylon and Silver staining Pierre Commercon
Red Cell Band 4.1 Dr. Brian M. Cooke
green fluorescent protein Michael Cooley
Tricks for pipetting detergents Dan Coovert
isolation of DNA from mouse tails Seth Corey
Yeast contamination on cell culture:HELP!! Joao Vasconcelos Costa
Antibodies to poly-His Maria C. Costanzo
long PCR kits Michael B. Coulthart
Southern Blotting of Pulse Field Gels.... HELP!!? Pascal Cousin
Differential display kits - recommendation please Vernon Coyne
DOW CHEMICAL JOINS GLOBAL CONSORTIUM ON ELECTRONIC LAB NOTEBOOKS CreateENBs at aol.com
DOW CHEMICAL JOINS GLOBAL CONSORTIUM ON ELECTRONIC LAB NOTEBOOKS CreateENBs at aol.com
Specificity of NEM toward cysteine residues Dominique DELFORGE
How to "normalize" DREDD
Primer Design software Homepage DREDD
Your basic stupid question about PCR cloning DREDD
Microcentrifuge preferences??? DSF
Tricks for pipetting detergents?? DSF
DNA electrophoresis R Daines
Qiagen refuses to tell! Richard Daines
Problems with ligation after GeneCleaning Nico Dantuma
(none) Martin Daoust
(none) Martin Daoust
Indicators for electroporation eficiency Levan Darjania
4+2 rule Robert Darnell
"Translabel" isotopes David L. Haviland, Ph.D.
Oligo-services... David L. Haviland, Ph.D.
PCR Not Allowed David L. Haviland, Ph.D.
hydropathy Oliver S. DePeyer
Qiagen: This is ridiculous Oliver S. DePeyer
Qiagen: This is ridiculous Oliver S. DePeyer
Reasons for PCR failure....? Tom Deits
How many kb in a centiMorgan?? Graham Dellaire
New at Experimental Medicine Job Listings!!! Graham Dellaire
Southern Blotting of Pulse Field Gels.... HELP!!? Graham Dellaire
Sliver staining DNA Jacqueline Delp
RNA-extraction from PAGE Detlef.Ritter at stud.uni-hannover.de
pGEX/GST gene fusion problems-> HELP! Michael DiDonato
pGEX/GST gene fusion problems-> HELP! Michael DiDonato
Smeary touchdown PCR gel. Dietmar
Continuous gel ref Paul Digard
Factor Xa Cleavage Paul Digard
Help with nuclease S1 protocol Dinakar
chloramphenicol John Dixon
RT-PCR difficulty - PLEASE HELP!!!!! John Dixon
what conc antibiotics for Carb/Kan/Tet cocktail? John Dixon
Biotin Pcr primers and anealing temperature John Dixon
Re- Cloning PCR Products Andrew J. Doherty
Cloning PCR products Dr Andrew J. Doherty
RNA Quantification... Colin Dolphin
more Hybond-N problems Gerald B. Downes
Genetrapper kit Dr N.J. Doyle
catalase/tissue culture Harold Drabkin
MluI cuts like it wants Richard J. Dudley
HELP!!! Fungal contamination of IMPORTANT transfectants! Richard J. Dudley
Yeast contamination on cell culture:HELP!! Richard J. Dudley
Where to buy pentosidine? KC Dunn
Northern Normalization Ken Dwyer
fungal genomic DNA EGUARINOS at MVAX.CBM.UAM.ES
UV crosslinking of DNA to proteins Simon Easterbrook-Smith
who makes 'OLIGO' software? - forget them! go with DNASTAR! Rubin Eitan
Absorbance spectrum for Xgal product Michael B. Elowitz
Grant Available - Genetic Engineering - Fungi Mark Emalfarb
Active Component in Skunk Urine Dianne Emslie
SYBR Green II RNA coloration Isabelle Ernest
pGEX/GST gene fusion problems-> HELP! Mark Evans
Evaluating reverse transcription Patrick HJ Falckh
RNA Centrifuge Spins Patrick HJ Falckh
Harris -80 Freezers Mark Farinha
Size fractionation of DNA Michael Feldman
looking for a method to strip gel plates Karl Fischer
Tricks for pipetting detergents?? Robert Fjellstrom
Uncouplers & pH L. Fliegel
Sequencing directly from gel slices ? Bryan L. Ford
Active Component in Skunk Urine Gerald Forrest
Total RNA labelling G.W.Phillips
Specificity of NEM toward cysteine residues Michael Galperin
N-terminal His-tag expression vector Susana Galvez
Chloropane ??? what the h... ??? Olivier Gandrillon
Loss of polymorphism Pooba Ganeshan
Comparing products Magazine Frederick Garbrecht
Your basic stupid question about PCR cloning Frederick Garbrecht
PCR variability with DNA extraction method Frederick Garbrecht
DNA gel extraction Frederick Garbrecht
Liposomal transfection into human hematopoetic stem cells Frederick Garbrecht
Permenant mounting of AP-NBT/BCIP slides Wes Garrett
standard for northern blot analysis Dr. Benoit Gauthier
RNA sizing against DNA markers Dr. Peter Gegenheimer
Toxic fragment in pBR322 Stephan Geley
What is: the toxic fragment in pBR322? Stephan Geley
Discrepancy between OD-value on spec and DNA on agarose gel Louis Geller
Problems with ligation after GeneCleaning Gabby Gerlitz
DNA contanimate Gabby Gerlitz
Help me! / Yeast transformation in Two-Hybrid screening Sourav Ghosh
bioluminescence-coelentrazine Luc Giasson
Carbenicillin Torben Gjetting
Help: genomic DNA purification Michelle Gleeson
DNA gel extraction Michelle Gleeson
removing fungal contamination from cell cultures Michelle Gleeson
LambdaGEM 11 Xho gLibrary Michelle Gleeson
looking for a method to strip gel plates Michelle Gleeson
Does Taq Stoffel fragment produce 3' A overhangs. Michelle Gleeson
NH4Ac-Miniprep becomes bad - why? Michelle Gleeson
software for DNA and Protein comparision Jack Goff
UV mutagenesis Steven Goldberg
Problems with ligation after GeneCleaning Steven Goldberg
pGEX/GST gene fusion problems-> HELP! Mark Gosink
Vector for direct cloning of PCR products Dr Ian Graham
Chlorampehical inhibit ssDNA packaging? Dr. James E. Graham
Using ClaI for subcloning Dr. James E. Graham
Taq polymerase patent (not) Dr. James E. Graham
sexing birds Dr. James E. Graham
freeze-squeeze (Re: geneclean: vortexing) Jim Graham
lysis of RAW macrophage-like cell line Jim Graham
Enumerating adherent cells (w/o harvest)? Jim Graham
Taq polymerase Jim Graham
Disinfectant contact t & Mycobacterium tuberculosis Jim Graham
Annealing of two complementary circular SS DNAs Lloyd Graham
antibody against human L1 L. Griffith
Freeze Shearing of DNA Steve Griffiths
AmpliTaq Gold vs AmpliTaq: where is the magic ? Steve Griffiths
Shearing genomic DNA Keith Grimaldi
geneclean: vortexing Charles M Griswold
PCR Not Allowed Duke Groebe
Biotin Pcr primers and anealing temperature Duke Groebe
Hybond N Problems Mike Gruidl
Hybond N Problems Mike Gruidl
DNA contanimate Halil-Cem Guersoy
Anyone tried Sypro Gel Stains??? Toumy Guettouche
glycerol to avoid protein precipitation?... Toumy Guettouche
NH4Ac-Miniprep becomes bad - why? Toumy Guettouche
RPA, protect bigger band than probe Xin Guo
poly-A purification Szabadkai Gyorgy
Help with DD RT-PCR?? Alex Haddad
Taq polymerase Alastair Hamilton
Reasons for PCR failure....? Gerald Hamilton
Help me! / Yeast transformation in Two-Hybrid screening Michael Hamilton
10 %FCS Clemens Oliver Hanemann
pGEX/GST gene fusion problems-> HELP! Brandon Harder
a good protocol for far westerns/overlays?? Gerald W. Hart
Source for 3'-dCTP??? Alex Harvey
M1/70 Ab and Western Blots 'Fumi' Fumitaka Hayashi
food colors in PCR Paul N Hengen
about transformation of DNA into E.Coli Paul N Hengen
Pointer to FAQ lists Paul N Hengen
Shearing genomic DNA Paul N Hengen
about transformation of DNA into E.Coli Paul N Hengen
Sliver staining DNA Paul N Hengen
Screening Libraries by PCR Paul N Hengen
about transformation of DNA into E.Coli Paul N Hengen
about transformation of DNA into E.Coli Paul N Hengen
Hybond N Problems Paul N Hengen
Glycogen for co-precipitating DNA Paul N Hengen
expressed protein is 5-6kda bigger than Paul N Hengen
DNA gel extraction Paul N Hengen
expressed protein is 5-6kda bigger than Paul N Hengen
Church buffers (Was Re: Hybond N) Paul N Hengen
pGEX/GST gene fusion problems-> HELP! Paul N Hengen
EMBO/ICGEB Phage Display Course Paul N Hengen
food colors in PCR Paul N. Hengen
Antibody to CKR5 ? Mike Herron
Recomend Fluor. Plate reader? Mike Herron
antibody to CKR5? Mike Herron
Has anyone separated proteins using agarose. John Hind
Westerns-blotting-BSA Robert Hindges
LambdaGEM 11 Xho gLibrary Carsten Hoege
MutS gel mobility shift Les Hoffman
UV Safe TAE Les Hoffman
Separating proteins on agarose Rosemary T. Hoffman
Reasons for PCR failure....? Susan Jane Hogarth
Reasons for PCR failure....? Susan Jane Hogarth
Qiagen: This is ridiculous Susan Jane Hogarth
Anyone tried Sypro Gel Stains??? Mike Holbrook
Insect cell electroporation Mike Holbrook
Spotty microsatellite sequencing gels:HELP (was: bind silane and sequencing) Paul Hollenbach
Continuous gel ref Louis Hom
Uncouplers & pH Louis Hom
peptide/antibody production companies Guido Hooiveld
endotoxin assay kit Jan Hoolwerf
RT primer for quantitative PCR Kathleen Hopp
GST-fusion expression problem Chris Houchens
Problems with ligation after GeneCleaning Chris Houchens
Annealing of two complementary circular SS DNAs Ken Howe
Seamless Cloning Carolyn Hoyal-Wrightson
Thermostable DNA polymerase can be used in Taq DNA ligase buffer? Mr. Chris Hoyle
expressed protein is 5-6kda bigger than Zheng Huanquan
expressed protein is 5-6kda bigger than Zheng Huanquan
Bacterially Expressed Proteins: IPTG induction Zheng Huanquan
Qiagen Quiaquick : reliable? pure enough for Autoseq? Matt Hudson
antibiotics for LBA4404 Matt Hudson
Westerns-blotting-BSA Matt Hudson
Oligonucleotide purification Lynn Hughes
Oligonucleotide purification Lynn Hughes
S-35 DNA and differential display Gene Huh
CAT gene -- fluorescent substrates? Gene Huh
looking for a method to strip gel plates Gene Huh
E.coli cultures lysing after induction Hong Hung
Discrepancy between OD-value on spec and DNA on agarose gel Jim Hutchins
Bind-Silane and sequencing Jim Hutchins
Has anyone separated proteins using agarose. Jim Hutchins
about thermocycling sequencing Jin-seon Im
about squeez-freeze Jin-seon Im
about Tag polymerase Jin-seon Im
about transformation of DNA into E.Coli Jin-seon Im
about competent cell Jin-seon Im
renaturating protein Jin-seon Im
some polymerase better than Tag? Jin-seon Im
bacterially expressed protein Jin-seon Im
blunt end cloning Jin-seon Im
Second hand zoo blots Imgenfm
Double stranded dna Sequencing help Immu-I
DNA extraction from BAC clone Hiroshi Inoue
Problem with mtDNA-Degradation Frederik Intelmann
Glycogen for co-precipitating DNA Frederik Intelmann
HELP!!! Lambda prep... Atsushi Isoai
Plasmid amplification in Cm-r E.coli Masayoshi Itoh
MICROEXTRACTION. Fraser Dr N J
MICROEXTRACTION. Fraser Dr N J
(none) Fraser Dr N J
RNA extraction Fraser Dr N J
(none) VASANTHARAJAN JANAKIRAMAN
(none) Thomas J. Jacks
Biotin Pcr primers and anealing temperature Timothy R Jackson
Oligonucleotide purification Daniel Jacobs
Looking for H. Bujard's Tet system plasmids: pBI-4 ... Fred Jacobs
Primer Design??? Fred Jacobs
Some polymerase better for PCR? Nick Jacobsen
Southern Blotting of Pulse Field Gels.... HELP!!? Nick Jacobsen
Genetic Analysis Linda St. James
"Biotech Rumor Mill" additions Linda St. James
Does Taq Stoffel fragment produce 3' A overhangs. Jamie
U.S.E. mutagenesis Jane.Wyatt.1 at massey.ac.nz
Qiagen: This is ridiculous/purity Richard A. Janssen
Looking for different CAT assay protocols ! Richard A. Janssen
Need Pyrimidine Dimer-DNA Glycosylase Scott Jelinsky
Qiagen DNA purification resin - what is is? Glenn Jenkins
Tricks for pipetting detergents?? Glenn Jenkins
Has anyone separated proteins using agarose. Glenn Jenkins
HELP!!! Fungal contamination of IMPORTANT transfectants! Glenn Jenkins
MCH in mammals Anabela Pinto Jensen
Active Component in Skunk Urine Dan Johnson
MOLCULAR WEIGHT MARKERS Doug Johnson
DNA size markers Dr. Douglas A. Johnson
Church buffers (Was Re: Hybond N) Mark Jordan
DNA contanimate Birgit Jostes
Pipette for PCR Birgit Jostes
Mung bean nuclease ? Fourie Joubert
re-using electroporation cuvettes Justinian
Cy5 Custom DNA Synthesis K.K.Chan
Test K.K.Chan
Test K.K.Chan
Cy5 Primers K.K.Chan
Cy5 Primers K.K.Chan
normalizing expression K.Kramer
AMBIONS ANTISENSE 18S TEMPLATE K.Kramer
sexing birds GERALDINE KAMINSKI
Roche AMPLICOR HCV PCR GERALDINE KAMINSKI
5 min transformation E. coli KHUANG at CHEMVX.TAMU.EDU
5 min transformation E. coli KHUANG at CHEMVX.TAMU.EDU
How to construct lab WWW page KHUANG at CHEMVX.TAMU.EDU
Sequencing PCR product by degenerateprimer KHUANG at CHEMVX.TAMU.EDU
Sliver staining DNA D. KIM
RNA sizing against DNA markers D. KIM
Mutagenesis: Does QuickChange (TM) work well? Stefan Kahlert
Need Mac NIH Grant Forms Alan Kaiser
Qiagen buffer contents Fredrik Kamme
Qiagen refuses to tell! Fredrik Kamme
Yeast cDNA bank in yeast vector? Aletta Karsies
Q. gel photo documentation systems. Ken Kaufman
gradient robocycler help Jill Kellogg
Need Help with Cos-7 Transfection and Transit Matthew Kennedy
Statistics and biology - a text book Nigel Kenward
Qiagen: This is ridiculous Nigel Kenward
Dig-riboprobes and RNase A Gabriele Kerber
Dig-riboprobes and RNase A Gabriele Kerber
RNA Purification Michael Kertesz
cDNA sequencing Islam Khan
Inserting cDNA into promoter constructs??? Obaid Y. Khan
Antibody contamination during immunoaffinity purification Dooyeon Kim
Tricks for pipetting detergents Sarah King
(none) Masato Kinoshita
U.S.E. mutagenesis Tim Kirksey
GST fusions forming dimers - reference, PLEASE Dima Klenchin
Cellulose-substrate for Chromatography Dima Klenchin
Detergent for Tissue Culture glassware Dima Klenchin
lower detection limit with Coomssie? Dima Klenchin
c600 David B. Knaebel
who makes 'OLIGO' software? Dave Knorr
X-gal staining without non-specific in the rat brain ? Riekeltje Koedood
Which company sell Maxam-Gilbert reaction kit? Riekeltje Koedood
What really indicates induction? Riekeltje Koedood
Transgenic reporter constructs Riekeltje Koedood
which phophoImager? Riekeltje Koedood
RAPD Trouble!!! Roland Koelliker
Possible reasons for RAPD failure Roland Koelliker
heme and binding Patrick Koppitz
Tricks for pipetting detergents?? Piotr Kozbial
about transformation of DNA into E.Coli Alexander Kraev
Church buffers (Was Re: Hybond N) Alexander Kraev
Sequencing directly from gel slices ? Alexander Kraev
Fluorescent Nucleotides Janice Kranz
Qiagen DNA purification resin - what is it? Cornelius Krasel
glycerol to avoid protein precipitation?... Cornelius Krasel
biotin labelling Joachim Kremerskothen
DEHYDRATED CULTURE MEDIA $18/LB David Kristofferson
Help with RNA extraction method Karen Kroeger
Cosmid isolation Dubear Kroening
Epitope tags: Methionines? Arle Kruckeberg
Primer Design??? Gary Kucera
Molecular biology software search Gary Kucera
Cloning PCR products Gary Kucera
RT-PCR Gary Kucera
Test, Please Ignore Gary Kucera
Ligating small insert Gary Kucera
Broad-range host for E.coli phages Peter Kuhnert
which phophoImager? Rork Kuick
truncated vector Alexander N. Kukushkin
Identification of a c-DNA clone Rajesh Kumar
DNA labelling Vivek Kumar
purity of urea for sequencing gels martin LEACH
Oligonucleotide purification martin LEACH
How many kb in a centiMorgan?? martin LEACH
Your basic stupid question about PCR cloning martin LEACH
sexing birds martin LEACH
How to construct lab WWW page martin LEACH
MedImmune INc anyone ?? martin LEACH
gradient robocycler help martin LEACH
(none) LIMARMG%BRUFMG.BITNET at UBVM.CC.BUFFALO.EDU
UV safe TAE ? Rico Laage
UV safe TAE ? Rico Laage
Qiagen: This is ridiculous Tsichlis Lab
Statistics and biology John Ladasky
PCR detection of HLA alleles John Ladasky
hnRNP isolation Michael Robert Ladomery
hybridization of expression lib James Warren Lafollette
Problems with ligation after GeneCleaning James Warren Lafollette
insect cell fusion Bill Langridge
Mung bean nuclease ? Karah Lanier
DNA-binding protein data file? Jean-Francois Larrivee
DNA-binding protein question Jean-Francois Larrivee
Anyone tried Sypro Gel Stains??? Michael Lassner
RNA Quantification... Edouard Lauzier
OD at 320nm Edouard Lauzier
who makes 'OLIGO' software? - forget them! go with DNASTAR! Andy Law
antibiotics for LBA4404 Byeong-ha Lee
carbenicillin Chang-Muk Lee
Antibodies to 6-His Tag Jongchan Lee
X-gal staining without non-specific in the rat brain ? Sukwon Lee
pCMV-LacZ ? Sukwon Lee
test Lee, Sun
unsubscribe Woo-Jai Lee
Tet-off/Tet-on system Philippe Lefebvre
DNA Mass Ladder problems Bettina Lehnhardt
RNA PURIFICATION Keith Leppard
Looking for different CAT assay protocols ! Keith Leppard
NH4Ac-Miniprep becomes bad - why? Keith Leppard
Remove SDS from protein preparation Keith Leppard
Tricks for pipetting detergents?? Wulf Dirk Leuschner
blunt end ligation Wulf Dirk Leuschner
Sections not sticking to slides Wulf Dirk Leuschner
expressed protein is 5-6kda bigger than Wulf Dirk Leuschner
Molecular biology software search Wulf Dirk Leuschner
Other minipreps (was Re: Qiagen: This is ridiculous) Victor Levenson
Selection with puromycin Victor Levenson
NH4Ac-Miniprep: The Protocol Victor Levenson
Problems with ligation after GeneCleaning Anis Limami
Problems with ligation after GeneCleaning Anis Limami
INFORMATION NEEDED -Plant Antioxidants- Anis Limami
HELP nuclear runon nuclear runoff Anis Limami
Cycle sequence results? Eric W. Linton
peptide syntheses Lisa Harrison-Bernard, PhD.
Pipette for PCR Chui Yiu Loon
Bind-Silane and sequencing LouPass
Microcentrifuge preferences??? LouPass
"Translabel" isotopes LouPass
Discrepancy between OD-value on spec and DNA on agarose gel Quentin E. Low
NIH-Image Blanca Lupiani
Looking for antibodies against Cre-recombinase Jennifer Lyon
plasmid curing M.De-Ungria at unsw.edu.au
DNA contanimate M.De-Ungria at unsw.edu.au
RT-PCR M.MECRIN
PCR INHIBITOR M.MECRIN
Antibodies to poly-His IAN MCFARLANE
Detergent for Tissue Culture glassware IAN MCFARLANE
Permenant mounting of AP-NBT/BCIP slides Paul C. Grimm MD
I Need Advice on single cell differential display Paul C. Grimm MD
Removal of Ethidium Bromide MELANSOND
Oligo-services... MELANSOND
about transformation of DNA into E.Coli MELANSOND
Discrepancy between OD-value on spec and DNA on agarose gel MELANSOND
No banding in CsCl Spin,Why? MELANSOND
Problems with ligation after GeneCleaning MELANSOND
Formaldehyde aliquots MELANSOND
Cleaning Quartz Cuvettes MELANSOND
DNA contanimate MELANSOND
looking for a method to strip gel plates MELANSOND
How to construct lab WWW page MMCCARTHY at BIOTECHNET.COM
Cholesterolfree FCS FABIAN MOEBIUS
XL-1 Red Mutagenesis FABIAN MOEBIUS
Flow Cytometry Protocols John MacDougall
Ribozyme construction John MacDougall
PCR variability with DNA extraction method Dr Claudia Macaubas
about transformation of DNA into E.Coli Hidevaldo Bueno Machado
blunt end ligation problems Morris Maduro
A problem in topology Rafael Maldonado
Discrepancy between OD-value on spec and DNA on agarose gel Rafael Maldonado
Bind-Silane and sequencing Rafael Maldonado
UV safe TAE ? Rafael Maldonado
Rules of thumb for sequencing cloned PCR products? Bryan John Maloney
Prodigisin Source? Stephen W. Mamber
Help with RNA extraction method Ned Mantei
Help with nuclease S1 protocol (long!) Ned Mantei
RPA, protect bigger band than probe Ned Mantei
Annealing of two complementary circular SS DNAs Ned Mantei
Annealing of two complementary circular SS DNAs Ned Mantei
Northern Normalization Ned Mantei
UV Safe TAE Ned Mantei
Double stranded dna Sequencing help Jeremy Marcus
Harris -80 Freezers Jeremy Marcus
about transformation of DNA into E.Coli Jeremy Marcus
Bind-Silane and sequencing Jeremy Marcus
No banding in CsCl Spin,Why? Jeremy Marcus
Problems with ligation after GeneCleaning Jeremy Marcus
Double stranded dna Sequencing help Mozart de Azevedo Marins
DNA analysis program Mozart de Azevedo Marins
U.S.E. mutagenesis Mark Brandt, Ph.D.
cleavage of MBP fusion proteins Mark Brandt, Ph.D.
Formaldehyde aliquots Paula Marquardt
Probes for rat ISH Amanda Marsh
Factor Xa Cleavage G E Martin
(none) Martin, Martin
Cosmids J. Pat Martinez
MICROEXTRACTION. Matthew P. Frosch, M.D., Ph.D.
pGEX/GST gene fusion problems-> HELP! Jeanne McAdara
Qiagen: This is ridiculous Jeanne McAdara
Reasons for PCR failure Susan B. McLeskey
CHO cells in suspension Ruth McMahon
cDNA visualization on agarose, HELP!!! Gerardo Medrano
OD at 320nm Jose E. Mejia
NH4Ac-Miniprep becomes bad - why? Jose E. Mejia
pCMVCAT Harutyan Melkonyan
I need a promoter selection vector, help Merja.Mikkonen at csc.fi
peptide/antibody production companies Pascal Mertens
gradient robocycler help MichaelCataniamcatania at ucla.edu
Contech Capfinder Michael_J_Hansbury
Active Component in Skunk Urine Edward Theodore Michelini
Bacterially Expressed Proteins: IPTG induction Ted Michelini
Using TdT to clean up sequencing reactions? David Micklem
Qiagen refuses to tell! David Micklem
Streck fixative for in situ Molecular Micro
HELP:Ab against LuECAM Thomas Mohr
human beta actin promoter in Euk exp. vector Richard Moldwin
Gene knockout in E.coli Andy Morby
Fugu and zebrafish DNA D.D. Morgan
Cy5 Custom DNA Synthesis Dr Michael Morris
Maxam-Gilbert sequencing ?! Dr Michael Morris
GENOMIC SOUTHERN: help with DNAseI Hypersensitivity (please!!!!!) (: Heidi Moss
Need Mac NIH Grant Forms Scott Moye-Rowley
Oligo-services... Frank Murphy
Bind-Silane and sequencing Frank Murphy
Qiagen: This is ridiculous Frank Murphy
Detecting HISx6 TJ Murphy
Toxic fragment in pBR322 Bernard Murray
Microcentrifuge preferences??? Bernard Murray
Re- PCR not allowed Bernard Murray
AmpliTaq Gold vs AmpliTaq: where is the magic ? Bernard Murray
chloramphenicol Bernard Murray
AMBIONS ANTISENSE 18S TEMPLATE Bernard Murray
Antibody contamination during immunoaffinity purification Bernard Murray
Westerns-blotting-BSA Bernard Murray
Molecular biology software search Bernard Murray
Northern Normalization Bernard Murray
RNA sizing against DNA markers Bernard Murray
Transgenic reporter constructs Bernard Murray
Rules of thumb for sequencing cloned PCR products? Bernard Murray
lobster genome size Michael Myers
Cosmids Peter J. Myler
How many Kb in a centiMorgan????Help Daniel Mytelka
Help me! / Yeast transformation in Two-Hybrid screening Takashi Nagano
Thrombin Cleavage of GST Lyle Najita
test Minh Q. Nguyen
test Minh Q. Nguyen
Ligating small insert Minh Q. Nguyen
about squeez-freeze Vince Nguyen
Trypsinization and NRO Vince Nguyen
Nuclear run-off with DIG-UTP Vince Nguyen
detection of PCR products by hydridization in streptoavidin coated plates Rick Nicholson
What really indicates induction? Rick Nicholson
HELP nuclear runon nuclear runoff Rick Nicholson
Antibody against c-jun Volker Noelle
MluI cuts like it wants Volker Noelle
Bacterially Expressed Proteins: IPTG induction Randy Nonay
which phophoImager? Randy Nonay
1997 FEBS Meeting on Cell Signalling Mechanisms Albert Noorlandt
blood spot extractions Norm
blood spots extractions Norm
oligo synthesizer - farewell!!! Norm
MOLCULAR WEIGHT MARKERS Pamela Norton
To Sailesh Takbum Ohn
Cre exprssing E. coli strain Yasushi Okazaki
pTrc99A-like plasmid... Pierre Olivier
PCR variability with DNA extraction method P R Orange
RAP PCR vs Differential display Sandra Pena De Ortiz
Transgenic reporter constructs Sandra Pena De Ortiz
Yeast contamination on cell culture:HELP!! Stephane Ory
Asparagine synthetase selection Masamichi Oshima
chloramphenicol P.Bundock
DIG-labelled RNA probes GILLIAN NI BHARLO BOTANY PG
Sections not sticking to slides GILLIAN NI BHARLO BOTANY PG
RNA sizing against DNA markers GILLIAN NI BHARLO BOTANY PG
detection of PCR products by hydridization in streptoavidin coated plates Alejandro Palacios
EIA detection of PCR products (streptoavidin capture) Jose Alejandro Palacios
A Test Manuel Palacios
Rules of thumb for sequencing cloned PCR products? Mark Pallen
DNA don't migrate... Jukka U Palo
SDS PAGE band blurring Inyup Park
Problems with ligation after GeneCleaning Yoonsuk Park
DNA Quantitation-Quick and Dirty Yoonsuk Park
Make Your Own Vacuum Pump for Gel Blotting! Robert Passey
Perkin-Elmer Taqman System Bharat Patel
Optimum conditions for Mitochondrial PCR Tara Paton
Northern Normalization Susan Patrick
What really indicates induction? Susan Patrick
inducible mammalian promoter Paul_J_Ryan
Concentrating proteins already in SDS sample buffer? D. Pearton
pGEX/GST gene fusion problems-> HELP! Pete
Kozak Sequence? Andy Peters
Test don't read Rodney Earl Pettway
test Rodney Earl Pettway
Carbos. in DNA prep!!!!!! Rodney Earl Pettway
LiCl in DNA prep Rodney Earl Pettway
Tricks for pipetting detergents?? Stephen P. Driska PhD
Spots on Northern probed with oligos (50-mers) Andy Phillips
Where is the gfp group Andy Phillips
Wedge Sequencing gels Andy Phillips
Q: E.coli and Inositol Lipids? C E Pical
(none) Venkat Pisupati
Copy number quantitation- PCR Daraporn Pittayakhachonwut
E.coli cultures lysing after induction Brent Pollock
Eliminate light/heavy chain after IP?? Andrei Popov
Looking for anti-CD9 and CD63 abs Yves Poumay
Detecting HISx6 Mark Prescott
glycerol to avoid protein precipitation?... Bill Prichett
DNA extraction from BAC clone Michael J. Prigge
HELP!!! Fungal contamination of IMPORTANT transfectants! Kiley R. Prilliman
gradient robocycler help Kiley R. Prilliman
Yeast contamination on cell culture:HELP!! Kiley R. Prilliman
about transformation of DNA into E.Coli ProZyme, Inc.
Cloning vector with kanamycin instead of ampicillin resistance ProZyme, Inc.
Size fractionation of DNA ProZyme, Inc.
Demo DNA ProZyme, Inc.
Yeast contamination on cell culture:HELP!! Xose S. Puente
unsubscribe REGCHAK at USTHK.UST.HK
about transformation of DNA into E.Coli H. ROYCHOWDHURY
about transformation of DNA into E.Coli H. ROYCHOWDHURY
Discrepancy between OD-value on spec and DNA on agarose gel H. ROYCHOWDHURY
Bind-Silane and sequencing H. ROYCHOWDHURY
Re- PCR not allowed H. ROYCHOWDHURY
Bind-Silane and sequencing H. ROYCHOWDHURY
LiCl in DNA prep H. ROYCHOWDHURY
looking for a method to strip gel plates H. ROYCHOWDHURY
NH4Ac-Miniprep becomes bad - why? H. ROYCHOWDHURY
silver staining... Christian Radauer
cloning in M13 Tony Raizis
help find peptide antisera service and information Vineet B Rajgarhia
DEHYDRATED CULTURE MEDIA $18/LB Robert Randolph
removing fungal contamination from cell cultures Robert Randolph
Help - problem with bFGF PCR! Rebecca
Cellulose-substrate for Chromatography Achim Recktenwald
SDS PAGE band blurring Achim Recktenwald
OD at 320nm Achim Recktenwald
glycerol to avoid protein precipitation?... Achim Recktenwald
glycerol to avoid protein precipitation?... Achim Recktenwald
Kinetics software Achim Recktenwald
DNA contanimate Achim Recktenwald
Reasons for PCR failure....? Martha L. Reed
Accession # for Human and Rat beta-globin gene Mary P. Remington
Delta RNA Fingerprinting Kit Mary P. Remington
Ligating small insert S.L. Rennie
Stains-all: Protein staining Scott Ripley.
lower detection limit with Coomssie? Bruce W. Ritchings
Antibodies to 6-His Tag Joseph Roland
Codons Usage software Francisco Uribe Romeo
sequencing MIcha Ron
Demo DNA Rosnthorn at aol.com
SV40 vs CMV promoter Marc Rosoff
Bacterially Expressed Proteins: IPTG induction Jay L. Rothstein
Microcentrifuge preferences??? Eric Roush
I need a PROTOCOL !!!! Rover
Molecular biology software search Eitan Rubin
Does a cDNA clone contain a full length???????? Eitan Rubin
Does a cDNA clone contain a full length???????? Eitan Rubin
Your basic stupid question about PCR cloning Eitan Rubin
4+2 rule Eitan Rubin
freeze-squeeze (Re: geneclean: vortexing) Eitan Rubin
Taq polymerase patent (not) Eitan Rubin
Reasons for PCR failure....? Eitan Rubin
RAPD Trouble!!! Eitan Rubin
Best prices of oligo synthesis - looking for details Eitan Rubin
Statistics and biology - a text book Eitan Rubin
purity of urea for sequencing gels George Rutherford
Reasons for PCR failure....? George Rutherford
Help with RNA extraction method George Rutherford
Hybond N Problems George Rutherford
BEMITHYL: ITS MECHANISM AND ITS EFFECTS STEMC
smear Northern Klaus Salger
Cloning vector with kanamycin instead of ampicillin resistance Klaus Salger
enolase Kamran Salim
Need help with CAT assays in bacteria Sarah K. Highlander, Ph.D.
Northern Normalization Heinz-Juergen Schaefers
Yeast contamination on cell culture:HELP!! Heinz-Juergen Schaefers
Problems with lipids Wolfgang Schechinger
European Radiochemical suppliers Wolfgang Schechinger
Q: Looking for GFP-cDNA Wolfgang Schechinger
Q: E.coli and Inositol Lipids? Wolfgang Schechinger
Where is the gfp group Wolfgang Schechinger
Q: E.coli and Inositol Lipids? Wolfgang Schechinger
Q: Looking for GFP-cDNA Wolfgang Schechinger
FITC fading kinetics Wolfgang Schechinger
Qiagen Quiaquick : reliable? pure enough for Autoseq? Wolfgang Schechinger
re-using electroporation cuvettes Claire Schimke
The Biotech BiblioNet Dyann K. Schmidel
EMSA problems Alexander Schmiechen
SDS PAGE bluring Denni Schnapp
pipetting detergents Denni Schnapp
RNA Sequencing Daniel R. Schoenberg
PRINS / midisat human DNA Manfred Schuermann
Tricks for pipetting detergents?? Bart Sefton
Invitrogen's pZERO & E.coli with ccdA Bret Sellman
Annealing of two complementary circular SS DNAs Robert Seymour
Other minipreps (was Re: Qiagen: This is ridiculous) Robert Seymour
Automated DNA Sequencing - better gel composition Sudarshan Sharma
?urine RT-PCR Dr. J.M. Sherwood
PCR variability with DNA extraction method benedicte Shevchenko
PCR variability with DNA extraction method benedicte Shevchenko
Reasons for PCR failure....? Joan Shields
looking for a method to strip gel plates Joan Shields
anti fungal GAPDH antibodies? Mari L. Shinohara
Problems with ligation after GeneCleaning Nori Shiraishi
Label incorporation into DNA in vivo Igor Sidorenkov
Cloning tandem repeats Richard Siegel
trehalose determination Sanford Silverman
PromoterFinder Construction Kit Gurmil Singh s/o Dalip Singh
Removing endotoxin from plasmids Mike Sklar
chemical mutagenesis Mark Slack
Seamless Cloning Dave Smith
RNA PURIFICATION Stephen Smith
blunt end ligation problems Wendy-Anne Smith
Tricks for pipetting detergents?? Gary Smyly
Cloning vector with kanamycin instead of ampicillin resistance Kimberley Snowden
Sections not sticking to slides Kimberley Snowden
Antibodies to poly-His Keld Sorensen
ELISA with 4-chloro-1-naphthol Keld Sorensen
gradient pcr? Keld Sorensen
SDS PAGE and phosphorylation Keld Sorensen
Westerns-blotting-BSA / E coli Ab Keld Sorensen
Detergent for Tissue Culture glassware Keld Sorensen
which imager? Keld Sorensen
smear Northern J. David Spafford
Harris -80 Freezers J. David Spafford
re-using electroporation cuvettes Ralf Spenneberg
sequence pTrcHis Ralf Spenneberg
NH4Ac-Miniprep becomes bad - why? Ralf Spenneberg
Which company sells Maxam-Gilbert reaction kit Dom Spinella
LiCl in DNA prep Dom Spinella
Rules of thumb for sequencing cloned PCR products Dom Spinella
PCR Detection of HLA Alleles Dom Spinella
RNA Sequencing Otello Stampacchia
WANTED: Yeast Library John Stebbins
Active Component in Skunk Urine Stefan Einar Stefansson
ConA - glycoproteins - ovalbumin Steve
Westerns-blotting-BSA Steve
Fiske and Subbarow reagent . . . Dr. Timothy Strabala
who makes 'OLIGO' software? Steven Sullivan
Reasons for PCR failure....? Sailesh Surapureddi
about transformation of DNA into E.Coli Sailesh Surapureddi
(none) Sailesh Surapureddi
DNA transformations into E.coli Sailesh Surapureddi
about competent cell Sailesh Surapureddi
5 min transformation E. coli Sailesh Surapureddi
5 min transformation E. coli Sailesh Surapureddi
Identification of a c-DNA clone Sailesh Surapureddi
who makes 'OLIGO' software? Sailesh Surapureddi
about transformation of DNA into E.Coli Sailesh Surapureddi
Double stranded dna Sequencing help Vlad Svetlov
Your basic stupid question about PCR cloning Vlad Svetlov
chemical mutagenesis Vlad Svetlov
No banding in CsCl Spin,Why? Vlad Svetlov
blunt end ligation problems Vladimir Svetlov
Microcentrifuge preferences??? Vladimir Svetlov
Some polymerase better for PCR? Vladimir Svetlov
Cloning PCR products Vladimir Svetlov
PCR primer selection software Vladimir Svetlov
Qiagen: This is ridiculous Vladimir Svetlov
Reasons for PCR failure....? Michal Svoboda
MedImmune INc anyone ?? Ganesh Jawahar Swaminathan
(no subject) Antoinette Sweeney
Chemifluorescence vs Chemiluminescence TPM
Genomic DNA prep Paul Tadich
dhfr activity assay? Steven Tait
GST fusions forming dimers - reference, PLEASE Song Tan
ANti human cytochrome b antibodies? Shiping Tang
Antibodies for human mt cytochrome b? Shiping Tang
Antibodies for human mt cytochrome b? Shiping Tang
pGex expression Ron Tate
ABI 310--help! Agnes Tay
AmpliTaq Gold vs AmpliTaq: where is the magic ? Dr G R Taylor
Kinetics software Cristiana M.G. Teixeira
Your basic stupid question about PCR cloning Chromosome Terror
Your basic stupid question about PCR cloning Chromosome Terror
I need a PROTOCOL !!!! Tom Thatcher
Epitope tags Steven Theg
Two hybrid library Steven Theg
U.S.E. mutagenesis Didier Thomas
GST-fusion expression problem Didier Thomas
FITC fading kinetics Didier Thomas
Shearing genomic DNA Michael W. Thompson
Cloning vector with kanamycin instead of ampicillin resistance Michael W. Thompson
Antibodies to 6-His Tag Michael W. Thompson
Tricks for pipetting detergents Michael W. Thompson
Hybond N Problems Michael W. Thompson
Detecting HISx6 Michael W. Thompson
Eukaryotic protein expression Michael W. Thompson
Eukaryotic protein expression Michael W. Thompson
DNA electrophoresis Michael W. Thompson
Qiagen refuses to tell! Michael W. Thompson
Qiagen refuses to tell! Michael W. Thompson
Selection with puromycin Michael W. Thompson
Qiagen refuses to tell! Michael W. Thompson
Looking for different CAT assay protocols ! Phillip Thompson
Antibodies to poly-His Pat Thoyts
Antibodies to 6-His Tag Pat Thoyts
SmaI digestion problems Rosemary Thresher
SmaI problems, correct address Rosemary Thresher
Test, please disregard Tony
BM non-radioactive probe labels? Robert J. Toonen
software for DNA and Protein comparision Giang Tran
pGex expression Giang Tran
Spectrophotometer Krzysztof Treder
Spectrophotometer Krzysztof Treder
(no subject) Otha Tripp
How to normalize Gary Truett
smear Northern James Tsuruta
Westerns-blotting-BSA James Tsuruta
I Need Advice on single cell differential display Douglas B. Tully
Sequence homology shareware Douglas B. Tully
Seamless Cloning->LIC (ligation independent cloning) Bradley Turner
Cloning tandem repeats Bradley Turner
Seamless Cloning->LIC (ligation independent cloning) Bradley Turner
Northern Normalization Przemko Tylzanowski
Oligo-services... Josh Udall
Sliver staining DNA Josh Udall
Q. gel photo documentation systems. Josh Udall
Size markers for sequencing gel Paul Ulanch
How many Kb in a centiMorgan????Help Paul Ulanch
PCR - SSCP loading mixture/denaturant?? Paul Ulanch
Purification of VLP«s Felix Unckell
Carbenicillin Dr. Derya Unutmaz
I need a PROTOCOL !!!! Virginia Urquidi
about transformation of DNA into E.Coli Edgar Valencia
Hi Dr. SaileshIce transformation question Edgar Valencia
UV crosslinking of DNA to proteins Luc Vallieres
endotoxin assay kit Marc Visconti
Discrepancy between OD-value on spec and DNA on agarose gel Botma Visser
Thrombin Cleavage of GST Dr A.H.M. Van Vliet
sequence pTrcHis Dr A.H.M. Van Vliet
Biotin Pcr primers and anealing temperature Karl Voss
Biotin Pcr primers and anealing temperature Karl Voss
DNA Quantitation-Quick and Dirty Karl Voss
Biotin Pcr primers and anealing temperature Karl Voss
Antibodies directed against interferon WENCENLAUS
Linscott alternative? WENCENLAUS
Linscott alternative? WENCENLAUS
al antibodies against soybean BBI needed WENCENLAUS
contextual expression analysis LMIB MULLERS WIL
MICROEXTRACTION Embryo Typing WSchick at aol.com
Recomend Fluor. Plate reader? WSchick at aol.com
PCR Not Allowed WSchick at aol.com
gradient robocycler time savings WSchick at aol.com
DNA dye for fluorometric quantitation WSchick at aol.com
Qiagen refuses to tell! Proprietary information. WSchick at aol.com
Tricks for pipetting detergents?? Paul Wakenight
Life on Mars Andrew Wallace
Recombinant ICE and CPP32 Andrew Wallace
Recombinant ICE and CPP32/Apopain Andrew Wallace
Recombinant ICE and Apopain/CPP32 Andrew Wallace
expressed protein is 5-6kda bigger than Andrew Wallace
Eukaryotic protein expression Katie Walstrom
Oligonucleotide purification Edward Wang
Mung bean nuclease ? Edward Wang
RAP PCR vs Differential display Edward Wang
RNA prep of Glomerular Core Edward Wang
Re- Cloning PCR Products S. Wang
expression vector S. Wang
Double stranded dna Sequencing help Shiao Wang
Some polymerase better for PCR? Shiao Wang
who makes 'OLIGO' software? - forget them! go with DNASTAR! Pearse Ward
DNA gel extraction Pearse Ward
Cellulose-substrate for Chromatography Nadia Wechsler
Help: genomic DNA purification Chi-kuang Wen
DNA gel extraction Jon West
pCMVCAT Dieter Wiesmann
In situ hybridization on tissue sections D. P. Wijesinghe
look for proof reading service William
YAC fragmentation vectors Gareth Williams
Reuse of Ni-columns? Troels Wind
lower detection limit with Coomssie? Troels Wind
Ni-chelation and Mg-binding Troels Wind
retroviral infection of lymphoblastoid cells Kimberly Ann Winkeler
Source for KlenTaq? Johannes Winkler
Experiences with HERMLE Microcentrifuges? Johannes Winkler
Large MW Standard f. SDSPAGE Christoph H. Winter
expressed protein is 5-6kda bigger than Christoph H. Winter
expressed protein is 5-6kda bigger than Christoph H. Winter
Accession # for Human and Rat beta-globin gene Harry Witchel
Cloning vector with kanamycin instead of ampicillin resistance Harry Witchel
Screening Libraries by PCR Harry Witchel
Disappearing plasmids Arthur Wohlwill
IsoQuick by Microprobe H. Woo
How to construct lab WWW page Jim Woodgett
Remove SDS from protein preparation Chao-Liang Wu
Q: PCR cloning from cDNA library Jinling Xie
ligation-mediated PCR H YANG
Reasons for PCR failure....? Mayumi Yagi
Rules of thumb for sequencing cloned PCR products? Mayumi Yagi
Vent polymerase from NEB Chin-Fen Yang
TEST Feng Yang
non-denaturing protein gel electrophoresis recipe? Feng Yang
blunt end ligation problems HY Yang
Detergent for Tissue Culture glassware George Yeung
Tricks for pipetting detergents?? H. Yim
Tetracycline inducible system Dong Yin
EBV/LMP-1 Chui Yiu-Loon
EBV/LMP-1 Chui Yiu-Loon
No banding in CsCl Spin,Why? Stephanie Yong
Searching for oleosin probe Stephanie Yong
Smeary touchdown PCR gel. Stephanie Yong
"Translabel" isotopes Ian A. York
Somatic cell hybridization Ian A. York
Qiagen: This is ridiculous Ian A. York
Qiagen: This is ridiculous Ian A. York
Ultracentrifuge experiences? Ian A. York
DNA gel extraction Taek H. You
DNA gel extraction Taek H. You
RNA PURIFICATION Taek H. You
Northern Normalization Taek H. You
RT-PCR difficulty - PLEASE HELP!!!!! Taek H. You
Help with Genomic PCR Taek H. You
First Base, free molecular biology software exclusively for OS/2 Taek H. You
LiCl in DNA prep Taek H. You
Sequencing directly from gel slices ? Taek H. You
RT-PCR difficulty - PLEASE HELP!!!!! Taek H. You
PKC assay in Hela cells David Tak Yan Yu
No banding in CsCl Spin,Why? Zhiyong Zhao
RNA Purification Zhiyong Zhao
problems with gel mobility shift assay Zhang Zhiying
Which company sell Maxam-Gilbert reaction kit? Fanxiu Zhu
DNA don't migrate... Guan Zhu
Thrombin Cleavage of GST Guan Zhu
whole mount in situ hybridization a101anne at horus.sara.nl
transcription termination ae at dna.bio.warwick.ac.uk
expressed protein is 5-6kda bigger than ae at dna.bio.warwick.ac.uk
Reasons for PCR failure....? jason affourtit
DNA dye for fluorometric quantitation alex at alfred.med.monash.edu.au
AmpliTaq Gold vs AmpliTaq: where is the magic ? bb1dopeg
AmpliTaq Gold vs AmpliTaq: where is the magic ? bb1dopeg
AmpliTaq Gold vs AmpliTaq: where is the magic ? bb1dopeg
yeast expression vector bhalerao
size of pBI 121? dinakar bhattramakki
DNA dye for fluorometric quantitation bigcheez at ma.ultranet.com
Lambda ZAP as a vector for bacterial libraries billing at vetsci.microvet.arizona.edu
expressed protein is 5-6kda bigger than billing at vetsci.microvet.arizona.edu
Cleaning Quartz Cuvettes billing at vetsci.microvet.arizona.edu
Carbos. in DNA prep!!!!!! billing at vetsci.microvet.arizona.edu
Temperature Sensitive pACYC or Derivatives billing at vetsci.microvet.arizona.edu
U937 cell extracts, retardation assay bodammer at mailer.uni-marburg.de
Affordable Patent Searching breffni at neca.com
SDS PAGE band blurring brett
No banding in CsCl Spin,Why? brett
Tricks for pipetting detergents?? brett
Seamless Cloning->LIC (ligation independent cloning) brett
Eukaryotic protein expression brett
Qiagen: This is ridiculous brett
Concentrating proteins already in SDS sample buffer? brett
Bpu10I enzyme carlo at florina.angers.inra.fr
transgenic mice carreer
transgenic mice carreer
I wish radioactive colloidal chitin!! choslab
CFTR mutation analysis Mike Morris sur cmusun12
Spotty microsatellite sequencing gels:HELP (was: bind silane and sequencing) craig.primmer at bmc.uu.se
How many Kb in a centiMorgan????Help dasuser at PO-Box.McGill.CA
Using TdT to clean up sequencing reactions? drdad at ktc.com
Using TdT to clean up sequencing reactions? drdad at ktc.com
subscription susan duthie
Using TdT to clean up sequencing reactions? edwardm at hhmi.ucla.edu
Cleaning Quartz Cuvettes edwardm at hhmi.ucla.edu
lysis of RAW macrophage-like cell line eheinecke at STUDENTS.WISC.EDU
DNA gel extraction ehwang at ucdavis.edu
(none) fotgar!fotgar.uba.ar!metodos at ccc.uba.ar
Qiagen Quiaquick : reliable? pure enough for Autoseq? futers at biovax.leeds.ac.uk
Protocol for Drosphila genomic DNA prep gc
pBEND anyone? gc
Dyestuff-biotech stain gem at pcnet.com
Probes for rat ISH kevin gilbride
Postdoc position in New Zealand available hraapg at marccri.marc.cri.nz
PCR - SSCP loading mixture/denaturant?? Administrador de iihema.fmed.uba.ar
GST-fusions and proteolysis ijiwaru at wheel.dcn.davis.ca.us
pGEX/GST gene fusion problems-> HELP! ijiwaru at wheel.dcn.davis.ca.us
Eukaryotic protein expression ijiwaru at wheel.dcn.davis.ca.us
Ligating small insert ijiwaru at wheel.dcn.davis.ca.us
RNA extraction Farid jalali
anti-heparin and EDTA PCR tricks jbw.hammond at ukonline.co.uk
does IPTG go bad??? jfh
IEC micromax jfh
renaturing proteins jgluz at STUD.MED.CORNELL.EDU
Vector for direct cloning of PCR products jlight at SICKKIDS.ON.CA
Eukaryotic protein expression jstrassw at OPAL.TUFTS.EDU
test jwobrien
Glycogen for co-precipitating DNA jwobrien
smear Northern kankaku
HELP HELP HELP needed with RNA extraction alex emil kentsis
Shearing genomic DNA Majumder lab
Sections not sticking to slides Majumder lab
expressed protein is 5-6kda bigger than lmcmurry_tet at opal.tufts.edu
normalizing expression lmrc.
Nucleotide Sequence of pTich5 takashi matsumoto
How determine target sequence of a novel DNA binding protein mcatania at ucla.edu
Beckman system gold nouveau hassane mchaourab
hemocyte marker? mhlee
How do I terminate transcription at a specific site in a plasmid that transfected into mammalian cells??? michael.baron at bbsrc.ac.uk
Oligo-services... milkman
Transgenic reporter constructs milstone
Harris -80 Freezers mpk6
Sequence homology shareware mpoulin at s-and-s.com
who makes 'OLIGO' software? - forget them! go with DNASTAR! mullenlynne at jsei.ucla.edu
CAT susanne müller
sexing birds nigel at mendel.berkeley.edu
Harris -80 Freezers nigel at mendel.berkeley.edu
Searching desperately for pJM17 (E1 adenovirus) noone
DIG Northerns nsaunders at molbiol.ox.ac.uk
PCR - SSCP loading mixture/denaturant?? alex parker
Direct Protection Assay Help... john phelan
DCOOD anhydrous: IS IT A DREAM? piero pollesello
BTX electroporator johanna preiszner
ISO: Next Plant Genome Meeting johanna preiszner
DNA Gel extraction pudwell at qut.edu.au
2-hybrid library for Candida albicans? john w. puziss
size of pBI 121? pzhang
antibiotics for LBA4404 pzhang
ref Quantitative PCR raconte at ibm.net
LiCl in DNA prep rcowherd at mail.med.upenn.edu
DNA don't migrate... rcowherd at mail.med.upenn.edu
Green Balancer ? reichhart at astorg.u-strasbg.fr
Green (GFP) Balancer (bis) ? reichhart at astorg.u-strasbg.fr
CAT gene albano renee
ssDNA->dsDNA transfos...was:annealing of ssDNA... rhubner at molbiol.ox.ac.uk
First Base, free molecular biology software exclusively for OS/2 rhughes at ucsd.edu
First Base, free molecular biology software exclusively for OS/2 rhughes at ucsd.edu
Staining gels in a microwave rjanssen_imm at opal.tufts.edu
about transformation of DNA into E.Coli jane sandall
chloramphenicol p.j. van santbrink
3' prime radiolabeling of oligo using dideoxynucleotides p.j. van santbrink
Molecular biology software search sczpjh at szn1.nott.ac.uk
Qiagen: This is ridiculous sharmaa at acsu.buffalo.edu
Test. Pls. ignore sharmaa at acsu.buffalo.edu
Hybond N Problems sunil shaw
dsRNA Killer Virus M probe sherif at DARWIN.UCSC.EDU
T-vector ssd2rc at po.iijnet.or.jp
Quantitation of individual nucleotides stein.bergan at labmed.uio.no
looking for a method to strip gel plates brad swanson
Looking for different CAT assay protocols ! th.neumann at dkfz-heidelberg.de
DNA electrophoresis u.381 at inserm.u-strasbg.fr
<None> varyc.mmcwp4 at mmc.org
gradient robocycler help wegloehner at mpimg-berlin-dahlem.mpg.de
PCR and food colors/dyes wick at cris.com
Please Help (pMAL) wsa
NIH-Image Hongyu yang
NIH-Image Hongyu yang
RAPD Trouble!!! Hongyu yang
Help: genomic DNA purification Hongyu yang
Need help with CAT assays in bacteria yugtuyg
who makes 'OLIGO' software? - forget them! go with DNASTAR! zinc
gradient robocycler help zinc
How to establish a stable pure line from transgenic plant? §Ñ¼~¯ó
Better method for deglycosylation...? Àå°ÇÈñ

Last message date: Sat Aug 31 15:12:12 EST 1996
Archived on: Sun Mar 6 21:10:22 EST 2005

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