Methods August 1996 Archives by author

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Starting: Thu Aug 1 00:34:52 EST 1996
Ending: Sat Aug 31 15:12:12 EST 1996
Messages: 1169

• who makes 'OLIGO' software? - forget them! go with DNASTAR!   "Stephen A. Bustin 3123", SURG
• reply: SSCP: Restriction Enzymes vs. Shorter Fragments   Gregory S. Buzard 'Buz'
• Reasons for PCR failure....?   Gregory S. Buzard 'Buz'
• PCR-SSCP loading mixture/denaturant??   Gregory S. Buzard 'Buz'
• Removal of Ethidium Bromide   Ed Seijo (BIO)
• Sequencing PCR product by degenerateprimer   *
• NH4Ac-Miniprep becomes bad - why?   *
• Patent on GeneClean?   *
• Patent on GeneClean?   *
• NH4Ac-Miniprep: The Protocol   *
• MICROEXTRACTION.  
• E. coli host strain for transformation   .69g
• Reasons for PCR failure....?   :-Peter
• E. coli host strain for transformation   ?=4~$^
• RNA PURIFICATION   MCCABE AJ
• RNA Purification   MCCABE AJ
• inducible mammalian promoter   NETSCAPE APPLICATION
• inducible mammalian promoter   NETSCAPE APPLICATION
• Antibodies to 6-His Tag   AShaw5
• expressed protein is 5-6kda bigger than   AShaw5
• Microcentrifuge preferences???   AShaw5
• Thrombin Cleavage of GST   AShaw5
• pGex expression   AShaw5
• Zoo blots   Mr Hassan Abdulrazzak
• Second hand zoo blots   Mr Hassan Abdulrazzak
• dhfr activity assay?   Shanthi Adimoolam
• BIOSCI/bionet miniFAQ & Fundraiser   BIOSCI Administrator
• blunt end ligation problems   Jeremy C. Ahouse
• puromycin selection in e. coli and lymphoblastoid cell lines (LCLs)   Ashok Aiyar
• Antibodies to 6-His Tag   Ashok Aiyar
• Cloning PCR products   Ashok Aiyar
• re-using electroporation cuvettes   Ashok Aiyar
• Selection with puromycin   Ashok Aiyar
• Selection with puromycin   Ashok Aiyar
• pBEND anyone?   Ashok Aiyar
• Help with DD RT-PCR??   Louis Alarcon
• myoglobin cDNA   Louis Alarcon
• Wanted: Mouse B-cell library   Shane Albright
• Gene knockout in E.coli   P.R. Alefounder
• Taq DNA ligase and PCR   P.R. Alefounder
• Taq data concealed?(was: Re-PCR not allowed)   Bill Alexander
• Removing endotoxin from plasmids   Mikhail Alexeyev
• size of pBI 121?   Mikhail Alexeyev
• Specificity of NEM toward cysteine residues   Mikhail Alexeyev
• Invitrogen's pZERO & E.coli with ccdA   Mikhail Alexeyev
• Invitrogen's pZERO & E.coli with ccdA   Mikhail Alexeyev
• Gene knockout in E.coli   Mikhail Alexeyev
• Need help with CAT assays in bacteria   Mikhail Alexeyev
• HELP!!! Lambda prep...   Allison
• Eliminate light/heavy chain after IP??   Danny Altschuler
• Selection with puromycin   Danny Altschuler
• GST fusions forming dimers - reference, PLEASE   Chen Ho An
• long PCR primer   Chen Ho An
• I need a PROTOCOL !!!!   Victor Anaya-Baez
• Looking for antibodies against Cre-recombinase   Thomas R. Anderson
• Antibodies to cre recombinase   Thomas R. Anderson
• anti-heparin and EDTA PCR tricks   Dan Andrews
• Biotin Pcr primers and anealing temperature   Dan Andrews
• Help:ss-DNA   Anja
• NIH Image advice...   Dr Alexander J Annala
• BioRad CHEF DR-II Pulsed Field Gel Electrophoresis Apparatus   Dr. Alexander J. Annala
• source of annexin   Joanna Aretz
• glycerol to avoid protein precipitation?...   Arioch
• Chloropane ??? what the h... ???   Josee Ash
• NH4Ac-Miniprep: The Protocol   Josee Ash
• E.coli cultures lysing after induction   Peter Ashton
• Position Open   Technical Assistance
• FITC fading kinetics   Graham Atherton
• DNA gel extraction   W R BENNETT
• Remove SDS from protein preparation   W R BENNETT
• GST-fusions and proteolysis   BIOTEC54
• Plasmid amplification in Cm-r E.coli   DAVE BOYD
• Help-Lg-scale Bacterial Genomic DNA Prep   Joel Bader
• Carbos. in DNA prep!!!!!!   Joel Bader
• CAT gene   Tim Barnett
• DNA Quantitation-Quick and Dirty   David Barrass
• cloning in M13   Kirk A Bartholomew
• cleavage of MBP fusion proteins   Guriq Basi
• subcloning pcr bands   L. Bastian
• Reasons for PCR failure....?   Jim Beasley
• A problem in topology   Ed Beaty
• DIG-labelled RNA probes   Ed Beaty
• CAT gene -- fluorescent substrates?   Ed Beaty
• UV safe TAE ?   Ed Beaty
• biotinylated nucleotide source?   Ed Beaty
• DNA-quantification by software   Andreas Becker
• Primer Design???   Andreas Becker
• Taq polymerase   Robin Beech
• PCR detection of HLA alleles   Robin Beech
• info on polyclonal antibodies against soybean BBI needed   Vadim Beilinson
• Re- Smeary Touchdown PCR   Brett Beitzel
• Re- PCR not allowed   Brett Beitzel
• Re- Amplitaq Gold   Brett Beitzel
• Re- Cloning PCR Products   Brett Beitzel
• Cheap Eporation Cuvettes?   Bob Belas
• trehalose determination   Ed Bellion
• Temperature Sensitive pACYC or Derivatives   Michael Benedik
• freeze-squeeze (Re: geneclean: vortexing)   Trevor Bezdek
• about transformation of DNA into E.Coli   Trevor Bezdek
• source for ICE or IchL   Trevor Bezdek
• Searching desperately for pJM17 (E1 adenovirus)   Trevor Bezdek
• Taq polymerase patent (not)   Tom Bickle
• vaccination with HIS tag protein   Stephen J Billington
• Cosmids   Stephen J Billington
• BioRad PROTEIN A BINDING BUFFER   Jeffrey Bjorge
• Harris -80 Freezers   Bruce Thomas Blakely
• Director Level Position   Martin Blankfard
• Cloning PCR products   Volker Blaschke
• Hela Cells and Adenylylcylase Components   Tracy Blevins
• Gram-positive sequence similarities-info req   Maria Bobrowska
• DNA electrophoresis   Maria Bobrowska
• B.napus genomic library   Peta C. Bonham-Smith
• Poly A Northern normalisation   Peta C. Bonham-Smith
• Annealing of two complementary circular SS DNAs   Joe Boutell
• Help me! / Yeast transformation in Two-Hybrid screening   Joe Boutell
• DNA contanimate   Joe Boutell
• Chloropane ??? what the h... ???   Robot Boy
• Annealing of two complementary circular SS DNAs   Chris Boyd
• Trizol   Susan Boyle-Vavra
• Help with PCR library screening   John Brennand
• Cloning vector with kanamycin instead of ampicillin resistance   John Brennand
• Size fractionation of DNA   John Brennand
• How do I terminate transcription at a specific site in a plasmid that transfected into mammalian cells???   Andrew Brooks
• HELP HELP HELP needed with RNA extraction   Paul A Bucciaglia
• How to "normalize"   Buddha
• Primer Design???   Buddha
• Thrombin Cleavage of GST   Evan Burkala
• Reasons for PCR failure....?   Bill Burnett
• Oligo-services...   Tonja Burshek
• RT-PCR difficulty - PLEASE HELP!!!!!   Tonja Burshek
• RT-PCR difficulty - PLEASE HELP!!!!!   Tonja Burshek
• catalase/tissue culture   Tim Buss
• Large MW Standard f. SDSPAGE   Dr E. Buxbaum
• Fiske and Subbarow reagent . . .   Dr E. Buxbaum
• Microcentrifuge preferences???   Dr E. Buxbaum
• Formaldehyde aliquots   Dr E. Buxbaum
• Tricks for pipetting detergents??   Dr E. Buxbaum
• glycerol to avoid protein precipitation?...   Dr E. Buxbaum
• Cleaning Quartz Cuvettes   Dr E. Buxbaum
• test: please, ignore me !   Christine CARTIER
• Custom BAC libraries?   STACY A CIUFO
• geneclean: vortexing   Manuel G. CLAROS
• HELP HELP HELP needed with RNA extraction   Manuel G. CLAROS
• How to denature target DNA on nylon membrane?   CRH
• Help with Genomic PCR   Miguel Cabrita
• Elongase vs. Expand: Which is better?   Miguel Cabrita
• ISO: Next Plant Genome Meeting   Roy G. Cantrell
• Where is the gfp group   Roberto Cappai
• endotoxin assay kit   Leslie Carvalho
• VVND   Leslie Carvalho
• Plasmid pKK3535?   Michael Casey
• BIOMETRA - Thermoblock ?   Michael Casey
• Where is the gfp group   Warren Casey
• Where is the gfp group   Warren Casey
• Statistics and biology   Edmundo Castro
• Glycogen for co-precipitating DNA   Edmundo Castro
• AMBIONS ANTISENSE 18S TEMPLATE   Jennifer L Causey
• AMBIONS ANTISENSE 18S TEMPLATE   Jennifer L Causey
• AMBIONS ANTISENSE 18S TEMPLATE   Jennifer L Causey
• Test - no message   Mike Challen
• Cy5 Custom DNA Synthesis   Dr Alan Chan
• Cy5 Custom DNA Synthesis   Dr Alan Chan
• Cy5 Custom DNA Synthesis   Dr Alan Chan
• BioRad PROTEIN A BINDING BUFFER   Alex Chang
• postivie control of HIV-1 Amplicor   Alex Chang
• How to denature target DNA on nylon membrane?   Jeremy Chen
• promoter screening   Leung Kwan Chi
• PCR primer selection software   LAU Tze Chin
• Carbos. in DNA prep!!!!!!   Tim Chipman
• Qiagen: This is ridiculous   Nikolai Chitaev
• Selection with puromycin   Nikolai Chitaev
• a good protocol for far westerns/overlays??   Nikolai Chitaev
• Your basic stupid question about PCR cloning   Dr. Duncan Clark
• Bind-Silane and sequencing   Dr. Duncan Clark
• Wedge Sequencing gels   Dr. Duncan Clark
• DNA size markers   Dr. Duncan Clark
• Mung bean nuclease ?   David Clarke
• postivie control of HIV-1 Amplicor   Dr. J.P. Clewley
• postivie control of HIV-1 Amplicor   Dr. J.P. Clewley
• pGEX/GST gene fusion problems-> HELP!   Steve Cohen
• Qiagen refuses to tell!   Steven Cohen
• Qiagen: This is ridiculous   Steven Cohen
• test   Allan Colthart
• RNA Quantification...   Allan Colthart
• Nylon and Silver staining   Pierre Commercon
• Red Cell Band 4.1   Dr. Brian M. Cooke
• green fluorescent protein   Michael Cooley
• Tricks for pipetting detergents   Dan Coovert
• isolation of DNA from mouse tails   Seth Corey
• Yeast contamination on cell culture:HELP!!   Joao Vasconcelos Costa
• Antibodies to poly-His   Maria C. Costanzo
• long PCR kits   Michael B. Coulthart
• Southern Blotting of Pulse Field Gels.... HELP!!?   Pascal Cousin
• Differential display kits - recommendation please   Vernon Coyne
• DOW CHEMICAL JOINS GLOBAL CONSORTIUM ON ELECTRONIC LAB NOTEBOOKS   CreateENBs at aol.com
• DOW CHEMICAL JOINS GLOBAL CONSORTIUM ON ELECTRONIC LAB NOTEBOOKS   CreateENBs at aol.com
• Specificity of NEM toward cysteine residues   Dominique DELFORGE
• How to "normalize"   DREDD
• Primer Design software Homepage   DREDD
• Your basic stupid question about PCR cloning   DREDD
• Microcentrifuge preferences???   DSF
• Tricks for pipetting detergents??   DSF
• DNA electrophoresis   R Daines
• Qiagen refuses to tell!   Richard Daines
• Problems with ligation after GeneCleaning   Nico Dantuma
• (none)   Martin Daoust
• (none)   Martin Daoust
• Indicators for electroporation eficiency   Levan Darjania
• 4+2 rule   Robert Darnell
• "Translabel" isotopes   David L. Haviland, Ph.D.
• Oligo-services...   David L. Haviland, Ph.D.
• PCR Not Allowed   David L. Haviland, Ph.D.
• hydropathy   Oliver S. DePeyer
• Qiagen: This is ridiculous   Oliver S. DePeyer
• Qiagen: This is ridiculous   Oliver S. DePeyer
• Reasons for PCR failure....?   Tom Deits
• How many kb in a centiMorgan??   Graham Dellaire
• New at Experimental Medicine Job Listings!!!   Graham Dellaire
• Southern Blotting of Pulse Field Gels.... HELP!!?   Graham Dellaire
• Sliver staining DNA   Jacqueline Delp
• RNA-extraction from PAGE   Detlef.Ritter at stud.uni-hannover.de
• pGEX/GST gene fusion problems-> HELP!   Michael DiDonato
• pGEX/GST gene fusion problems-> HELP!   Michael DiDonato
• Smeary touchdown PCR gel.   Dietmar
• Continuous gel ref   Paul Digard
• Factor Xa Cleavage   Paul Digard
• Help with nuclease S1 protocol   Dinakar
• chloramphenicol   John Dixon
• RT-PCR difficulty - PLEASE HELP!!!!!   John Dixon
• what conc antibiotics for Carb/Kan/Tet cocktail?   John Dixon
• Biotin Pcr primers and anealing temperature   John Dixon
• Re- Cloning PCR Products   Andrew J. Doherty
• Cloning PCR products   Dr Andrew J. Doherty
• RNA Quantification...   Colin Dolphin
• more Hybond-N problems   Gerald B. Downes
• Genetrapper kit   Dr N.J. Doyle
• catalase/tissue culture   Harold Drabkin
• MluI cuts like it wants   Richard J. Dudley
• HELP!!! Fungal contamination of IMPORTANT transfectants!   Richard J. Dudley
• Yeast contamination on cell culture:HELP!!   Richard J. Dudley
• Where to buy pentosidine?   KC Dunn
• Northern Normalization   Ken Dwyer
• fungal genomic DNA   EGUARINOS at MVAX.CBM.UAM.ES
• UV crosslinking of DNA to proteins   Simon Easterbrook-Smith
• who makes 'OLIGO' software? - forget them! go with DNASTAR!   Rubin Eitan
• Absorbance spectrum for Xgal product   Michael B. Elowitz
• Grant Available - Genetic Engineering - Fungi   Mark Emalfarb
• Active Component in Skunk Urine   Dianne Emslie
• SYBR Green II RNA coloration   Isabelle Ernest
• pGEX/GST gene fusion problems-> HELP!   Mark Evans
• Evaluating reverse transcription   Patrick HJ Falckh
• RNA Centrifuge Spins   Patrick HJ Falckh
• Harris -80 Freezers   Mark Farinha
• Size fractionation of DNA   Michael Feldman
• looking for a method to strip gel plates   Karl Fischer
• Tricks for pipetting detergents??   Robert Fjellstrom
• Uncouplers & pH   L. Fliegel
• Sequencing directly from gel slices ?   Bryan L. Ford
• Active Component in Skunk Urine   Gerald Forrest
• Total RNA labelling   G.W.Phillips
• Specificity of NEM toward cysteine residues   Michael Galperin
• N-terminal His-tag expression vector   Susana Galvez
• Chloropane ??? what the h... ???   Olivier Gandrillon
• Loss of polymorphism   Pooba Ganeshan
• Comparing products Magazine   Frederick Garbrecht
• Your basic stupid question about PCR cloning   Frederick Garbrecht
• PCR variability with DNA extraction method   Frederick Garbrecht
• DNA gel extraction   Frederick Garbrecht
• Liposomal transfection into human hematopoetic stem cells   Frederick Garbrecht
• Permenant mounting of AP-NBT/BCIP slides   Wes Garrett
• standard for northern blot analysis   Dr. Benoit Gauthier
• RNA sizing against DNA markers   Dr. Peter Gegenheimer
• Toxic fragment in pBR322   Stephan Geley
• What is: the toxic fragment in pBR322?   Stephan Geley
• Discrepancy between OD-value on spec and DNA on agarose gel   Louis Geller
• Problems with ligation after GeneCleaning   Gabby Gerlitz
• DNA contanimate   Gabby Gerlitz
• Help me! / Yeast transformation in Two-Hybrid screening   Sourav Ghosh
• bioluminescence-coelentrazine   Luc Giasson
• Carbenicillin   Torben Gjetting
• Help: genomic DNA purification   Michelle Gleeson
• DNA gel extraction   Michelle Gleeson
• removing fungal contamination from cell cultures   Michelle Gleeson
• LambdaGEM 11 Xho gLibrary   Michelle Gleeson
• looking for a method to strip gel plates   Michelle Gleeson
• Does Taq Stoffel fragment produce 3' A overhangs.   Michelle Gleeson
• NH4Ac-Miniprep becomes bad - why?   Michelle Gleeson
• software for DNA and Protein comparision   Jack Goff
• UV mutagenesis   Steven Goldberg
• Problems with ligation after GeneCleaning   Steven Goldberg
• pGEX/GST gene fusion problems-> HELP!   Mark Gosink
• Vector for direct cloning of PCR products   Dr Ian Graham
• Chlorampehical inhibit ssDNA packaging?   Dr. James E. Graham
• Using ClaI for subcloning   Dr. James E. Graham
• Taq polymerase patent (not)   Dr. James E. Graham
• sexing birds   Dr. James E. Graham
• freeze-squeeze (Re: geneclean: vortexing)   Jim Graham
• lysis of RAW macrophage-like cell line   Jim Graham
• Enumerating adherent cells (w/o harvest)?   Jim Graham
• Taq polymerase   Jim Graham
• Disinfectant contact t & Mycobacterium tuberculosis   Jim Graham
• Annealing of two complementary circular SS DNAs   Lloyd Graham
• antibody against human L1   L. Griffith
• Freeze Shearing of DNA   Steve Griffiths
• AmpliTaq Gold vs AmpliTaq: where is the magic ?   Steve Griffiths
• Shearing genomic DNA   Keith Grimaldi
• geneclean: vortexing   Charles M Griswold
• PCR Not Allowed   Duke Groebe
• Biotin Pcr primers and anealing temperature   Duke Groebe
• Hybond N Problems   Mike Gruidl
• Hybond N Problems   Mike Gruidl
• DNA contanimate   Halil-Cem Guersoy
• Anyone tried Sypro Gel Stains???   Toumy Guettouche
• glycerol to avoid protein precipitation?...   Toumy Guettouche
• NH4Ac-Miniprep becomes bad - why?   Toumy Guettouche
• RPA, protect bigger band than probe   Xin Guo
• poly-A purification   Szabadkai Gyorgy
• Help with DD RT-PCR??   Alex Haddad
• Taq polymerase   Alastair Hamilton
• Reasons for PCR failure....?   Gerald Hamilton
• Help me! / Yeast transformation in Two-Hybrid screening   Michael Hamilton
• 10 %FCS   Clemens Oliver Hanemann
• pGEX/GST gene fusion problems-> HELP!   Brandon Harder
• a good protocol for far westerns/overlays??   Gerald W. Hart
• Source for 3'-dCTP???   Alex Harvey
• M1/70 Ab and Western Blots   'Fumi' Fumitaka Hayashi
• food colors in PCR   Paul N Hengen
• about transformation of DNA into E.Coli   Paul N Hengen
• Pointer to FAQ lists   Paul N Hengen
• Shearing genomic DNA   Paul N Hengen
• about transformation of DNA into E.Coli   Paul N Hengen
• Sliver staining DNA   Paul N Hengen
• Screening Libraries by PCR   Paul N Hengen
• about transformation of DNA into E.Coli   Paul N Hengen
• about transformation of DNA into E.Coli   Paul N Hengen
• Hybond N Problems   Paul N Hengen
• Glycogen for co-precipitating DNA   Paul N Hengen
• expressed protein is 5-6kda bigger than   Paul N Hengen
• DNA gel extraction   Paul N Hengen
• expressed protein is 5-6kda bigger than   Paul N Hengen
• Church buffers (Was Re: Hybond N)   Paul N Hengen
• pGEX/GST gene fusion problems-> HELP!   Paul N Hengen
• EMBO/ICGEB Phage Display Course   Paul N Hengen
• food colors in PCR   Paul N. Hengen
• Antibody to CKR5 ?   Mike Herron
• Recomend Fluor. Plate reader?   Mike Herron
• antibody to CKR5?   Mike Herron
• Has anyone separated proteins using agarose.   John Hind
• Westerns-blotting-BSA   Robert Hindges
• LambdaGEM 11 Xho gLibrary   Carsten Hoege
• MutS gel mobility shift   Les Hoffman
• UV Safe TAE   Les Hoffman
• Separating proteins on agarose   Rosemary T. Hoffman
• Reasons for PCR failure....?   Susan Jane Hogarth
• Reasons for PCR failure....?   Susan Jane Hogarth
• Qiagen: This is ridiculous   Susan Jane Hogarth
• Anyone tried Sypro Gel Stains???   Mike Holbrook
• Insect cell electroporation   Mike Holbrook
• Spotty microsatellite sequencing gels:HELP (was: bind silane and sequencing)   Paul Hollenbach
• Continuous gel ref   Louis Hom
• Uncouplers & pH   Louis Hom
• peptide/antibody production companies   Guido Hooiveld
• endotoxin assay kit   Jan Hoolwerf
• RT primer for quantitative PCR   Kathleen Hopp
• GST-fusion expression problem   Chris Houchens
• Problems with ligation after GeneCleaning   Chris Houchens
• Annealing of two complementary circular SS DNAs   Ken Howe
• Seamless Cloning   Carolyn Hoyal-Wrightson
• Thermostable DNA polymerase can be used in Taq DNA ligase buffer?   Mr. Chris Hoyle
• expressed protein is 5-6kda bigger than   Zheng Huanquan
• expressed protein is 5-6kda bigger than   Zheng Huanquan
• Bacterially Expressed Proteins: IPTG induction   Zheng Huanquan
• Qiagen Quiaquick : reliable? pure enough for Autoseq?   Matt Hudson
• antibiotics for LBA4404   Matt Hudson
• Westerns-blotting-BSA   Matt Hudson
• Oligonucleotide purification   Lynn Hughes
• Oligonucleotide purification   Lynn Hughes
• S-35 DNA and differential display   Gene Huh
• CAT gene -- fluorescent substrates?   Gene Huh
• looking for a method to strip gel plates   Gene Huh
• E.coli cultures lysing after induction   Hong Hung
• Discrepancy between OD-value on spec and DNA on agarose gel   Jim Hutchins
• Bind-Silane and sequencing   Jim Hutchins
• Has anyone separated proteins using agarose.   Jim Hutchins
• about thermocycling sequencing   Jin-seon Im
• about squeez-freeze   Jin-seon Im
• about Tag polymerase   Jin-seon Im
• about transformation of DNA into E.Coli   Jin-seon Im
• about competent cell   Jin-seon Im
• renaturating protein   Jin-seon Im
• some polymerase better than Tag?   Jin-seon Im
• bacterially expressed protein   Jin-seon Im
• blunt end cloning   Jin-seon Im
• Second hand zoo blots   Imgenfm
• Double stranded dna Sequencing help   Immu-I
• DNA extraction from BAC clone   Hiroshi Inoue
• Problem with mtDNA-Degradation   Frederik Intelmann
• Glycogen for co-precipitating DNA   Frederik Intelmann
• HELP!!! Lambda prep...   Atsushi Isoai
• Plasmid amplification in Cm-r E.coli   Masayoshi Itoh
• MICROEXTRACTION.   Fraser Dr N J
• MICROEXTRACTION.   Fraser Dr N J
• (none)   Fraser Dr N J
• RNA extraction   Fraser Dr N J
• (none)   VASANTHARAJAN JANAKIRAMAN
• (none)   Thomas J. Jacks
• Biotin Pcr primers and anealing temperature   Timothy R Jackson
• Oligonucleotide purification   Daniel Jacobs
• Looking for H. Bujard's Tet system plasmids: pBI-4 ...   Fred Jacobs
• Primer Design???   Fred Jacobs
• Some polymerase better for PCR?   Nick Jacobsen
• Southern Blotting of Pulse Field Gels.... HELP!!?   Nick Jacobsen
• Genetic Analysis   Linda St. James
• "Biotech Rumor Mill" additions   Linda St. James
• Does Taq Stoffel fragment produce 3' A overhangs.   Jamie
• U.S.E. mutagenesis   Jane.Wyatt.1 at massey.ac.nz
• Qiagen: This is ridiculous/purity   Richard A. Janssen
• Looking for different CAT assay protocols !   Richard A. Janssen
• Need Pyrimidine Dimer-DNA Glycosylase   Scott Jelinsky
• Qiagen DNA purification resin - what is is?   Glenn Jenkins
• Tricks for pipetting detergents??   Glenn Jenkins
• Has anyone separated proteins using agarose.   Glenn Jenkins
• HELP!!! Fungal contamination of IMPORTANT transfectants!   Glenn Jenkins
• MCH in mammals   Anabela Pinto Jensen
• Active Component in Skunk Urine   Dan Johnson
• MOLCULAR WEIGHT MARKERS   Doug Johnson
• DNA size markers   Dr. Douglas A. Johnson
• Church buffers (Was Re: Hybond N)   Mark Jordan
• DNA contanimate   Birgit Jostes
• Pipette for PCR   Birgit Jostes
• Mung bean nuclease ?   Fourie Joubert
• re-using electroporation cuvettes   Justinian
• Cy5 Custom DNA Synthesis   K.K.Chan
• Test   K.K.Chan
• Test   K.K.Chan
• Cy5 Primers   K.K.Chan
• Cy5 Primers   K.K.Chan
• normalizing expression   K.Kramer
• AMBIONS ANTISENSE 18S TEMPLATE   K.Kramer
• sexing birds   GERALDINE KAMINSKI
• Roche AMPLICOR HCV PCR   GERALDINE KAMINSKI
• 5 min transformation E. coli   KHUANG at CHEMVX.TAMU.EDU
• 5 min transformation E. coli   KHUANG at CHEMVX.TAMU.EDU
• How to construct lab WWW page   KHUANG at CHEMVX.TAMU.EDU
• Sequencing PCR product by degenerateprimer   KHUANG at CHEMVX.TAMU.EDU
• Sliver staining DNA   D. KIM
• RNA sizing against DNA markers   D. KIM
• Mutagenesis: Does QuickChange (TM) work well?   Stefan Kahlert
• Need Mac NIH Grant Forms   Alan Kaiser
• Qiagen buffer contents   Fredrik Kamme
• Qiagen refuses to tell!   Fredrik Kamme
• Yeast cDNA bank in yeast vector?   Aletta Karsies
• Q. gel photo documentation systems.   Ken Kaufman
• gradient robocycler help   Jill Kellogg
• Need Help with Cos-7 Transfection and Transit   Matthew Kennedy
• Statistics and biology - a text book   Nigel Kenward
• Qiagen: This is ridiculous   Nigel Kenward
• Dig-riboprobes and RNase A   Gabriele Kerber
• Dig-riboprobes and RNase A   Gabriele Kerber
• RNA Purification   Michael Kertesz
• cDNA sequencing   Islam Khan
• Inserting cDNA into promoter constructs???   Obaid Y. Khan
• Antibody contamination during immunoaffinity purification   Dooyeon Kim
• Tricks for pipetting detergents   Sarah King
• (none)   Masato Kinoshita
• U.S.E. mutagenesis   Tim Kirksey
• GST fusions forming dimers - reference, PLEASE   Dima Klenchin
• Cellulose-substrate for Chromatography   Dima Klenchin
• Detergent for Tissue Culture glassware   Dima Klenchin
• lower detection limit with Coomssie?   Dima Klenchin
• c600   David B. Knaebel
• who makes 'OLIGO' software?   Dave Knorr
• X-gal staining without non-specific in the rat brain ?   Riekeltje Koedood
• Which company sell Maxam-Gilbert reaction kit?   Riekeltje Koedood
• What really indicates induction?   Riekeltje Koedood
• Transgenic reporter constructs   Riekeltje Koedood
• which phophoImager?   Riekeltje Koedood
• RAPD Trouble!!!   Roland Koelliker
• Possible reasons for RAPD failure   Roland Koelliker
• heme and binding   Patrick Koppitz
• Tricks for pipetting detergents??   Piotr Kozbial
• about transformation of DNA into E.Coli   Alexander Kraev
• Church buffers (Was Re: Hybond N)   Alexander Kraev
• Sequencing directly from gel slices ?   Alexander Kraev
• Fluorescent Nucleotides   Janice Kranz
• Qiagen DNA purification resin - what is it?   Cornelius Krasel
• glycerol to avoid protein precipitation?...   Cornelius Krasel
• biotin labelling   Joachim Kremerskothen
• DEHYDRATED CULTURE MEDIA $18/LB   David Kristofferson
• Help with RNA extraction method   Karen Kroeger
• Cosmid isolation   Dubear Kroening
• Epitope tags: Methionines?   Arle Kruckeberg
• Primer Design???   Gary Kucera
• Molecular biology software search   Gary Kucera
• Cloning PCR products   Gary Kucera
• RT-PCR   Gary Kucera
• Test, Please Ignore   Gary Kucera
• Ligating small insert   Gary Kucera
• Broad-range host for E.coli phages   Peter Kuhnert
• which phophoImager?   Rork Kuick
• truncated vector   Alexander N. Kukushkin
• Identification of a c-DNA clone   Rajesh Kumar
• DNA labelling   Vivek Kumar
• purity of urea for sequencing gels   martin LEACH
• Oligonucleotide purification   martin LEACH
• How many kb in a centiMorgan??   martin LEACH
• Your basic stupid question about PCR cloning   martin LEACH
• sexing birds   martin LEACH
• How to construct lab WWW page   martin LEACH
• MedImmune INc anyone ??   martin LEACH
• gradient robocycler help   martin LEACH
• (none)   LIMARMG%BRUFMG.BITNET at UBVM.CC.BUFFALO.EDU
• UV safe TAE ?   Rico Laage
• UV safe TAE ?   Rico Laage
• Qiagen: This is ridiculous   Tsichlis Lab
• Statistics and biology   John Ladasky
• PCR detection of HLA alleles   John Ladasky
• hnRNP isolation   Michael Robert Ladomery
• hybridization of expression lib   James Warren Lafollette
• Problems with ligation after GeneCleaning   James Warren Lafollette
• insect cell fusion   Bill Langridge
• Mung bean nuclease ?   Karah Lanier
• DNA-binding protein data file?   Jean-Francois Larrivee
• DNA-binding protein question   Jean-Francois Larrivee
• Anyone tried Sypro Gel Stains???   Michael Lassner
• RNA Quantification...   Edouard Lauzier
• OD at 320nm   Edouard Lauzier
• who makes 'OLIGO' software? - forget them! go with DNASTAR!   Andy Law
• antibiotics for LBA4404   Byeong-ha Lee
• carbenicillin   Chang-Muk Lee
• Antibodies to 6-His Tag   Jongchan Lee
• X-gal staining without non-specific in the rat brain ?   Sukwon Lee
• pCMV-LacZ ?   Sukwon Lee
• test   Lee, Sun
• unsubscribe   Woo-Jai Lee
• Tet-off/Tet-on system   Philippe Lefebvre
• DNA Mass Ladder problems   Bettina Lehnhardt
• RNA PURIFICATION   Keith Leppard
• Looking for different CAT assay protocols !   Keith Leppard
• NH4Ac-Miniprep becomes bad - why?   Keith Leppard
• Remove SDS from protein preparation   Keith Leppard
• Tricks for pipetting detergents??   Wulf Dirk Leuschner
• blunt end ligation   Wulf Dirk Leuschner
• Sections not sticking to slides   Wulf Dirk Leuschner
• expressed protein is 5-6kda bigger than   Wulf Dirk Leuschner
• Molecular biology software search   Wulf Dirk Leuschner
• Other minipreps (was Re: Qiagen: This is ridiculous)   Victor Levenson
• Selection with puromycin   Victor Levenson
• NH4Ac-Miniprep: The Protocol   Victor Levenson
• Problems with ligation after GeneCleaning   Anis Limami
• Problems with ligation after GeneCleaning   Anis Limami
• INFORMATION NEEDED -Plant Antioxidants-   Anis Limami
• HELP nuclear runon nuclear runoff   Anis Limami
• Cycle sequence results?   Eric W. Linton
• peptide syntheses   Lisa Harrison-Bernard, PhD.
• Pipette for PCR   Chui Yiu Loon
• Bind-Silane and sequencing   LouPass
• Microcentrifuge preferences???   LouPass
• "Translabel" isotopes   LouPass
• Discrepancy between OD-value on spec and DNA on agarose gel   Quentin E. Low
• NIH-Image   Blanca Lupiani
• Looking for antibodies against Cre-recombinase   Jennifer Lyon
• plasmid curing   M.De-Ungria at unsw.edu.au
• DNA contanimate   M.De-Ungria at unsw.edu.au
• RT-PCR   M.MECRIN
• PCR INHIBITOR   M.MECRIN
• Antibodies to poly-His   IAN MCFARLANE
• Detergent for Tissue Culture glassware   IAN MCFARLANE
• Permenant mounting of AP-NBT/BCIP slides   Paul C. Grimm MD
• I Need Advice on single cell differential display   Paul C. Grimm MD
• Removal of Ethidium Bromide   MELANSOND
• Oligo-services...   MELANSOND
• about transformation of DNA into E.Coli   MELANSOND
• Discrepancy between OD-value on spec and DNA on agarose gel   MELANSOND
• No banding in CsCl Spin,Why?   MELANSOND
• Problems with ligation after GeneCleaning   MELANSOND
• Formaldehyde aliquots   MELANSOND
• Cleaning Quartz Cuvettes   MELANSOND
• DNA contanimate   MELANSOND
• looking for a method to strip gel plates   MELANSOND
• How to construct lab WWW page   MMCCARTHY at BIOTECHNET.COM
• Cholesterolfree FCS   FABIAN MOEBIUS
• XL-1 Red Mutagenesis   FABIAN MOEBIUS
• Flow Cytometry Protocols   John MacDougall
• Ribozyme construction   John MacDougall
• PCR variability with DNA extraction method   Dr Claudia Macaubas
• about transformation of DNA into E.Coli   Hidevaldo Bueno Machado
• blunt end ligation problems   Morris Maduro
• A problem in topology   Rafael Maldonado
• Discrepancy between OD-value on spec and DNA on agarose gel   Rafael Maldonado
• Bind-Silane and sequencing   Rafael Maldonado
• UV safe TAE ?   Rafael Maldonado
• Rules of thumb for sequencing cloned PCR products?   Bryan John Maloney
• Prodigisin Source?   Stephen W. Mamber
• Help with RNA extraction method   Ned Mantei
• Help with nuclease S1 protocol (long!)   Ned Mantei
• RPA, protect bigger band than probe   Ned Mantei
• Annealing of two complementary circular SS DNAs   Ned Mantei
• Annealing of two complementary circular SS DNAs   Ned Mantei
• Northern Normalization   Ned Mantei
• UV Safe TAE   Ned Mantei
• Double stranded dna Sequencing help   Jeremy Marcus
• Harris -80 Freezers   Jeremy Marcus
• about transformation of DNA into E.Coli   Jeremy Marcus
• Bind-Silane and sequencing   Jeremy Marcus
• No banding in CsCl Spin,Why?   Jeremy Marcus
• Problems with ligation after GeneCleaning   Jeremy Marcus
• Double stranded dna Sequencing help   Mozart de Azevedo Marins
• DNA analysis program   Mozart de Azevedo Marins
• U.S.E. mutagenesis   Mark Brandt, Ph.D.
• cleavage of MBP fusion proteins   Mark Brandt, Ph.D.
• Formaldehyde aliquots   Paula Marquardt
• Probes for rat ISH   Amanda Marsh
• Factor Xa Cleavage   G E Martin
• (none)   Martin, Martin
• Cosmids   J. Pat Martinez
• MICROEXTRACTION.   Matthew P. Frosch, M.D., Ph.D.
• pGEX/GST gene fusion problems-> HELP!   Jeanne McAdara
• Qiagen: This is ridiculous   Jeanne McAdara
• Reasons for PCR failure   Susan B. McLeskey
• CHO cells in suspension   Ruth McMahon
• cDNA visualization on agarose, HELP!!!   Gerardo Medrano
• OD at 320nm   Jose E. Mejia
• NH4Ac-Miniprep becomes bad - why?   Jose E. Mejia
• pCMVCAT   Harutyan Melkonyan
• I need a promoter selection vector, help   Merja.Mikkonen at csc.fi
• peptide/antibody production companies   Pascal Mertens
• gradient robocycler help   MichaelCataniamcatania at ucla.edu
• Contech Capfinder   Michael_J_Hansbury
• Active Component in Skunk Urine   Edward Theodore Michelini
• Bacterially Expressed Proteins: IPTG induction   Ted Michelini
• Using TdT to clean up sequencing reactions?   David Micklem
• Qiagen refuses to tell!   David Micklem
• Streck fixative for in situ   Molecular Micro
• HELP:Ab against LuECAM   Thomas Mohr
• human beta actin promoter in Euk exp. vector   Richard Moldwin
• Gene knockout in E.coli   Andy Morby
• Fugu and zebrafish DNA   D.D. Morgan
• Cy5 Custom DNA Synthesis   Dr Michael Morris
• Maxam-Gilbert sequencing ?!   Dr Michael Morris
• GENOMIC SOUTHERN: help with DNAseI Hypersensitivity (please!!!!!) (:   Heidi Moss
• Need Mac NIH Grant Forms   Scott Moye-Rowley
• Oligo-services...   Frank Murphy
• Bind-Silane and sequencing   Frank Murphy
• Qiagen: This is ridiculous   Frank Murphy
• Detecting HISx6   TJ Murphy
• Toxic fragment in pBR322   Bernard Murray
• Microcentrifuge preferences???   Bernard Murray
• Re- PCR not allowed   Bernard Murray
• AmpliTaq Gold vs AmpliTaq: where is the magic ?   Bernard Murray
• chloramphenicol   Bernard Murray
• AMBIONS ANTISENSE 18S TEMPLATE   Bernard Murray
• Antibody contamination during immunoaffinity purification   Bernard Murray
• Westerns-blotting-BSA   Bernard Murray
• Molecular biology software search   Bernard Murray
• Northern Normalization   Bernard Murray
• RNA sizing against DNA markers   Bernard Murray
• Transgenic reporter constructs   Bernard Murray
• Rules of thumb for sequencing cloned PCR products?   Bernard Murray
• lobster genome size   Michael Myers
• Cosmids   Peter J. Myler
• How many Kb in a centiMorgan????Help   Daniel Mytelka
• Help me! / Yeast transformation in Two-Hybrid screening   Takashi Nagano
• Thrombin Cleavage of GST   Lyle Najita
• test   Minh Q. Nguyen
• test   Minh Q. Nguyen
• Ligating small insert   Minh Q. Nguyen
• about squeez-freeze   Vince Nguyen
• Trypsinization and NRO   Vince Nguyen
• Nuclear run-off with DIG-UTP   Vince Nguyen
• detection of PCR products by hydridization in streptoavidin coated plates   Rick Nicholson
• What really indicates induction?   Rick Nicholson
• HELP nuclear runon nuclear runoff   Rick Nicholson
• Antibody against c-jun   Volker Noelle
• MluI cuts like it wants   Volker Noelle
• Bacterially Expressed Proteins: IPTG induction   Randy Nonay
• which phophoImager?   Randy Nonay
• 1997 FEBS Meeting on Cell Signalling Mechanisms   Albert Noorlandt
• blood spot extractions   Norm
• blood spots extractions   Norm
• oligo synthesizer - farewell!!!   Norm
• MOLCULAR WEIGHT MARKERS   Pamela Norton
• To Sailesh   Takbum Ohn
• Cre exprssing E. coli strain   Yasushi Okazaki
• pTrc99A-like plasmid...   Pierre Olivier
• PCR variability with DNA extraction method   P R Orange
• RAP PCR vs Differential display   Sandra Pena De Ortiz
• Transgenic reporter constructs   Sandra Pena De Ortiz
• Yeast contamination on cell culture:HELP!!   Stephane Ory
• Asparagine synthetase selection   Masamichi Oshima
• chloramphenicol   P.Bundock
• DIG-labelled RNA probes   GILLIAN NI BHARLO BOTANY PG
• Sections not sticking to slides   GILLIAN NI BHARLO BOTANY PG
• RNA sizing against DNA markers   GILLIAN NI BHARLO BOTANY PG
• detection of PCR products by hydridization in streptoavidin coated plates   Alejandro Palacios
• EIA detection of PCR products (streptoavidin capture)   Jose Alejandro Palacios
• A Test   Manuel Palacios
• Rules of thumb for sequencing cloned PCR products?   Mark Pallen
• DNA don't migrate...   Jukka U Palo
• SDS PAGE band blurring   Inyup Park
• Problems with ligation after GeneCleaning   Yoonsuk Park
• DNA Quantitation-Quick and Dirty   Yoonsuk Park
• Make Your Own Vacuum Pump for Gel Blotting!   Robert Passey
• Perkin-Elmer Taqman System   Bharat Patel
• Optimum conditions for Mitochondrial PCR   Tara Paton
• Northern Normalization   Susan Patrick
• What really indicates induction?   Susan Patrick
• inducible mammalian promoter   Paul_J_Ryan
• Concentrating proteins already in SDS sample buffer?   D. Pearton
• pGEX/GST gene fusion problems-> HELP!   Pete
• Kozak Sequence?   Andy Peters
• Test don't read   Rodney Earl Pettway
• test   Rodney Earl Pettway
• Carbos. in DNA prep!!!!!!   Rodney Earl Pettway
• LiCl in DNA prep   Rodney Earl Pettway
• Tricks for pipetting detergents??   Stephen P. Driska PhD
• Spots on Northern probed with oligos (50-mers)   Andy Phillips
• Where is the gfp group   Andy Phillips
• Wedge Sequencing gels   Andy Phillips
• Q: E.coli and Inositol Lipids?   C E Pical
• (none)   Venkat Pisupati
• Copy number quantitation- PCR   Daraporn Pittayakhachonwut
• E.coli cultures lysing after induction   Brent Pollock
• Eliminate light/heavy chain after IP??   Andrei Popov
• Looking for anti-CD9 and CD63 abs   Yves Poumay
• Detecting HISx6   Mark Prescott
• glycerol to avoid protein precipitation?...   Bill Prichett
• DNA extraction from BAC clone   Michael J. Prigge
• HELP!!! Fungal contamination of IMPORTANT transfectants!   Kiley R. Prilliman
• gradient robocycler help   Kiley R. Prilliman
• Yeast contamination on cell culture:HELP!!   Kiley R. Prilliman
• about transformation of DNA into E.Coli   ProZyme, Inc.
• Cloning vector with kanamycin instead of ampicillin resistance   ProZyme, Inc.
• Size fractionation of DNA   ProZyme, Inc.
• Demo DNA   ProZyme, Inc.
• Yeast contamination on cell culture:HELP!!   Xose S. Puente
• unsubscribe   REGCHAK at USTHK.UST.HK
• about transformation of DNA into E.Coli   H. ROYCHOWDHURY
• about transformation of DNA into E.Coli   H. ROYCHOWDHURY
• Discrepancy between OD-value on spec and DNA on agarose gel   H. ROYCHOWDHURY
• Bind-Silane and sequencing   H. ROYCHOWDHURY
• Re- PCR not allowed   H. ROYCHOWDHURY
• Bind-Silane and sequencing   H. ROYCHOWDHURY
• LiCl in DNA prep   H. ROYCHOWDHURY
• looking for a method to strip gel plates   H. ROYCHOWDHURY
• NH4Ac-Miniprep becomes bad - why?   H. ROYCHOWDHURY
• silver staining...   Christian Radauer
• cloning in M13   Tony Raizis
• help find peptide antisera service and information   Vineet B Rajgarhia
• DEHYDRATED CULTURE MEDIA $18/LB   Robert Randolph
• removing fungal contamination from cell cultures   Robert Randolph
• Help - problem with bFGF PCR!   Rebecca
• Cellulose-substrate for Chromatography   Achim Recktenwald
• SDS PAGE band blurring   Achim Recktenwald
• OD at 320nm   Achim Recktenwald
• glycerol to avoid protein precipitation?...   Achim Recktenwald
• glycerol to avoid protein precipitation?...   Achim Recktenwald
• Kinetics software   Achim Recktenwald
• DNA contanimate   Achim Recktenwald
• Reasons for PCR failure....?   Martha L. Reed
• Accession # for Human and Rat beta-globin gene   Mary P. Remington
• Delta RNA Fingerprinting Kit   Mary P. Remington
• Ligating small insert   S.L. Rennie
• Stains-all: Protein staining   Scott Ripley.
• lower detection limit with Coomssie?   Bruce W. Ritchings
• Antibodies to 6-His Tag   Joseph Roland
• Codons Usage software   Francisco Uribe Romeo
• sequencing   MIcha Ron
• Demo DNA   Rosnthorn at aol.com
• SV40 vs CMV promoter   Marc Rosoff
• Bacterially Expressed Proteins: IPTG induction   Jay L. Rothstein
• Microcentrifuge preferences???   Eric Roush
• I need a PROTOCOL !!!!   Rover
• Molecular biology software search   Eitan Rubin
• Does a cDNA clone contain a full length????????   Eitan Rubin
• Does a cDNA clone contain a full length????????   Eitan Rubin
• Your basic stupid question about PCR cloning   Eitan Rubin
• 4+2 rule   Eitan Rubin
• freeze-squeeze (Re: geneclean: vortexing)   Eitan Rubin
• Taq polymerase patent (not)   Eitan Rubin
• Reasons for PCR failure....?   Eitan Rubin
• RAPD Trouble!!!   Eitan Rubin
• Best prices of oligo synthesis - looking for details   Eitan Rubin
• Statistics and biology - a text book   Eitan Rubin
• purity of urea for sequencing gels   George Rutherford
• Reasons for PCR failure....?   George Rutherford
• Help with RNA extraction method   George Rutherford
• Hybond N Problems   George Rutherford
• BEMITHYL: ITS MECHANISM AND ITS EFFECTS   STEMC
• smear Northern   Klaus Salger
• Cloning vector with kanamycin instead of ampicillin resistance   Klaus Salger
• enolase   Kamran Salim
• Need help with CAT assays in bacteria   Sarah K. Highlander, Ph.D.
• Northern Normalization   Heinz-Juergen Schaefers
• Yeast contamination on cell culture:HELP!!   Heinz-Juergen Schaefers
• Problems with lipids   Wolfgang Schechinger
• European Radiochemical suppliers   Wolfgang Schechinger
• Q: Looking for GFP-cDNA   Wolfgang Schechinger
• Q: E.coli and Inositol Lipids?   Wolfgang Schechinger
• Where is the gfp group   Wolfgang Schechinger
• Q: E.coli and Inositol Lipids?   Wolfgang Schechinger
• Q: Looking for GFP-cDNA   Wolfgang Schechinger
• FITC fading kinetics   Wolfgang Schechinger
• Qiagen Quiaquick : reliable? pure enough for Autoseq?   Wolfgang Schechinger
• re-using electroporation cuvettes   Claire Schimke
• The Biotech BiblioNet   Dyann K. Schmidel
• EMSA problems   Alexander Schmiechen
• SDS PAGE bluring   Denni Schnapp
• pipetting detergents   Denni Schnapp
• RNA Sequencing   Daniel R. Schoenberg
• PRINS / midisat human DNA   Manfred Schuermann
• Tricks for pipetting detergents??   Bart Sefton
• Invitrogen's pZERO & E.coli with ccdA   Bret Sellman
• Annealing of two complementary circular SS DNAs   Robert Seymour
• Other minipreps (was Re: Qiagen: This is ridiculous)   Robert Seymour
• Automated DNA Sequencing - better gel composition   Sudarshan Sharma
• ?urine RT-PCR   Dr. J.M. Sherwood
• PCR variability with DNA extraction method   benedicte Shevchenko
• PCR variability with DNA extraction method   benedicte Shevchenko
• Reasons for PCR failure....?   Joan Shields
• looking for a method to strip gel plates   Joan Shields
• anti fungal GAPDH antibodies?   Mari L. Shinohara
• Problems with ligation after GeneCleaning   Nori Shiraishi
• Label incorporation into DNA in vivo   Igor Sidorenkov
• Cloning tandem repeats   Richard Siegel
• trehalose determination   Sanford Silverman
• PromoterFinder Construction Kit   Gurmil Singh s/o Dalip Singh
• Removing endotoxin from plasmids   Mike Sklar
• chemical mutagenesis   Mark Slack
• Seamless Cloning   Dave Smith
• RNA PURIFICATION   Stephen Smith
• blunt end ligation problems   Wendy-Anne Smith
• Tricks for pipetting detergents??   Gary Smyly
• Cloning vector with kanamycin instead of ampicillin resistance   Kimberley Snowden
• Sections not sticking to slides   Kimberley Snowden
• Antibodies to poly-His   Keld Sorensen
• ELISA with 4-chloro-1-naphthol   Keld Sorensen
• gradient pcr?   Keld Sorensen
• SDS PAGE and phosphorylation   Keld Sorensen
• Westerns-blotting-BSA / E coli Ab   Keld Sorensen
• Detergent for Tissue Culture glassware   Keld Sorensen
• which imager?   Keld Sorensen
• smear Northern   J. David Spafford
• Harris -80 Freezers   J. David Spafford
• re-using electroporation cuvettes   Ralf Spenneberg
• sequence pTrcHis   Ralf Spenneberg
• NH4Ac-Miniprep becomes bad - why?   Ralf Spenneberg
• Which company sells Maxam-Gilbert reaction kit   Dom Spinella
• LiCl in DNA prep   Dom Spinella
• Rules of thumb for sequencing cloned PCR products   Dom Spinella
• PCR Detection of HLA Alleles   Dom Spinella
• RNA Sequencing   Otello Stampacchia
• WANTED: Yeast Library   John Stebbins
• Active Component in Skunk Urine   Stefan Einar Stefansson
• ConA - glycoproteins - ovalbumin   Steve
• Westerns-blotting-BSA   Steve
• Fiske and Subbarow reagent . . .   Dr. Timothy Strabala
• who makes 'OLIGO' software?   Steven Sullivan
• Reasons for PCR failure....?   Sailesh Surapureddi
• about transformation of DNA into E.Coli   Sailesh Surapureddi
• (none)   Sailesh Surapureddi
• DNA transformations into E.coli   Sailesh Surapureddi
• about competent cell   Sailesh Surapureddi
• 5 min transformation E. coli   Sailesh Surapureddi
• 5 min transformation E. coli   Sailesh Surapureddi
• Identification of a c-DNA clone   Sailesh Surapureddi
• who makes 'OLIGO' software?   Sailesh Surapureddi
• about transformation of DNA into E.Coli   Sailesh Surapureddi
• Double stranded dna Sequencing help   Vlad Svetlov
• Your basic stupid question about PCR cloning   Vlad Svetlov
• chemical mutagenesis   Vlad Svetlov
• No banding in CsCl Spin,Why?   Vlad Svetlov
• blunt end ligation problems   Vladimir Svetlov
• Microcentrifuge preferences???   Vladimir Svetlov
• Some polymerase better for PCR?   Vladimir Svetlov
• Cloning PCR products   Vladimir Svetlov
• PCR primer selection software   Vladimir Svetlov
• Qiagen: This is ridiculous   Vladimir Svetlov
• Reasons for PCR failure....?   Michal Svoboda
• MedImmune INc anyone ??   Ganesh Jawahar Swaminathan
• (no subject)   Antoinette Sweeney
• Chemifluorescence vs Chemiluminescence   TPM
• Genomic DNA prep   Paul Tadich
• dhfr activity assay?   Steven Tait
• GST fusions forming dimers - reference, PLEASE   Song Tan
• ANti human cytochrome b antibodies?   Shiping Tang
• Antibodies for human mt cytochrome b?   Shiping Tang
• Antibodies for human mt cytochrome b?   Shiping Tang
• pGex expression   Ron Tate
• ABI 310--help!   Agnes Tay
• AmpliTaq Gold vs AmpliTaq: where is the magic ?   Dr G R Taylor
• Kinetics software   Cristiana M.G. Teixeira
• Your basic stupid question about PCR cloning   Chromosome Terror
• Your basic stupid question about PCR cloning   Chromosome Terror
• I need a PROTOCOL !!!!   Tom Thatcher
• Epitope tags   Steven Theg
• Two hybrid library   Steven Theg
• U.S.E. mutagenesis   Didier Thomas
• GST-fusion expression problem   Didier Thomas
• FITC fading kinetics   Didier Thomas
• Shearing genomic DNA   Michael W. Thompson
• Cloning vector with kanamycin instead of ampicillin resistance   Michael W. Thompson
• Antibodies to 6-His Tag   Michael W. Thompson
• Tricks for pipetting detergents   Michael W. Thompson
• Hybond N Problems   Michael W. Thompson
• Detecting HISx6   Michael W. Thompson
• Eukaryotic protein expression   Michael W. Thompson
• Eukaryotic protein expression   Michael W. Thompson
• DNA electrophoresis   Michael W. Thompson
• Qiagen refuses to tell!   Michael W. Thompson
• Qiagen refuses to tell!   Michael W. Thompson
• Selection with puromycin   Michael W. Thompson
• Qiagen refuses to tell!   Michael W. Thompson
• Looking for different CAT assay protocols !   Phillip Thompson
• Antibodies to poly-His   Pat Thoyts
• Antibodies to 6-His Tag   Pat Thoyts
• SmaI digestion problems   Rosemary Thresher
• SmaI problems, correct address   Rosemary Thresher
• Test, please disregard   Tony
• BM non-radioactive probe labels?   Robert J. Toonen
• software for DNA and Protein comparision   Giang Tran
• pGex expression   Giang Tran
• Spectrophotometer   Krzysztof Treder
• Spectrophotometer   Krzysztof Treder
• (no subject)   Otha Tripp
• How to normalize   Gary Truett
• smear Northern   James Tsuruta
• Westerns-blotting-BSA   James Tsuruta
• I Need Advice on single cell differential display   Douglas B. Tully
• Sequence homology shareware   Douglas B. Tully
• Seamless Cloning->LIC (ligation independent cloning)   Bradley Turner
• Cloning tandem repeats   Bradley Turner
• Seamless Cloning->LIC (ligation independent cloning)   Bradley Turner
• Northern Normalization   Przemko Tylzanowski
• Oligo-services...   Josh Udall
• Sliver staining DNA   Josh Udall
• Q. gel photo documentation systems.   Josh Udall
• Size markers for sequencing gel   Paul Ulanch
• How many Kb in a centiMorgan????Help   Paul Ulanch
• PCR - SSCP loading mixture/denaturant??   Paul Ulanch
• Purification of VLP«s   Felix Unckell
• Carbenicillin   Dr. Derya Unutmaz
• I need a PROTOCOL !!!!   Virginia Urquidi
• about transformation of DNA into E.Coli   Edgar Valencia
• Hi Dr. SaileshIce transformation question   Edgar Valencia
• UV crosslinking of DNA to proteins   Luc Vallieres
• endotoxin assay kit   Marc Visconti
• Discrepancy between OD-value on spec and DNA on agarose gel   Botma Visser
• Thrombin Cleavage of GST   Dr A.H.M. Van Vliet
• sequence pTrcHis   Dr A.H.M. Van Vliet
• Biotin Pcr primers and anealing temperature   Karl Voss
• Biotin Pcr primers and anealing temperature   Karl Voss
• DNA Quantitation-Quick and Dirty   Karl Voss
• Biotin Pcr primers and anealing temperature   Karl Voss
• Antibodies directed against interferon   WENCENLAUS
• Linscott alternative?   WENCENLAUS
• Linscott alternative?   WENCENLAUS
• al antibodies against soybean BBI needed   WENCENLAUS
• contextual expression analysis   LMIB MULLERS WIL
• MICROEXTRACTION Embryo Typing   WSchick at aol.com
• Recomend Fluor. Plate reader?   WSchick at aol.com
• PCR Not Allowed   WSchick at aol.com
• gradient robocycler time savings   WSchick at aol.com
• DNA dye for fluorometric quantitation   WSchick at aol.com
• Qiagen refuses to tell! Proprietary information.   WSchick at aol.com
• Tricks for pipetting detergents??   Paul Wakenight
• Life on Mars   Andrew Wallace
• Recombinant ICE and CPP32   Andrew Wallace
• Recombinant ICE and CPP32/Apopain   Andrew Wallace
• Recombinant ICE and Apopain/CPP32   Andrew Wallace
• expressed protein is 5-6kda bigger than   Andrew Wallace
• Eukaryotic protein expression   Katie Walstrom
• Oligonucleotide purification   Edward Wang
• Mung bean nuclease ?   Edward Wang
• RAP PCR vs Differential display   Edward Wang
• RNA prep of Glomerular Core   Edward Wang
• Re- Cloning PCR Products   S. Wang
• expression vector   S. Wang
• Double stranded dna Sequencing help   Shiao Wang
• Some polymerase better for PCR?   Shiao Wang
• who makes 'OLIGO' software? - forget them! go with DNASTAR!   Pearse Ward
• DNA gel extraction   Pearse Ward
• Cellulose-substrate for Chromatography   Nadia Wechsler
• Help: genomic DNA purification   Chi-kuang Wen
• DNA gel extraction   Jon West
• pCMVCAT   Dieter Wiesmann
• In situ hybridization on tissue sections   D. P. Wijesinghe
• look for proof reading service   William
• YAC fragmentation vectors   Gareth Williams
• Reuse of Ni-columns?   Troels Wind
• lower detection limit with Coomssie?   Troels Wind
• Ni-chelation and Mg-binding   Troels Wind
• retroviral infection of lymphoblastoid cells   Kimberly Ann Winkeler
• Source for KlenTaq?   Johannes Winkler
• Experiences with HERMLE Microcentrifuges?   Johannes Winkler
• Large MW Standard f. SDSPAGE   Christoph H. Winter
• expressed protein is 5-6kda bigger than   Christoph H. Winter
• expressed protein is 5-6kda bigger than   Christoph H. Winter
• Accession # for Human and Rat beta-globin gene   Harry Witchel
• Cloning vector with kanamycin instead of ampicillin resistance   Harry Witchel
• Screening Libraries by PCR   Harry Witchel
• Disappearing plasmids   Arthur Wohlwill
• IsoQuick by Microprobe   H. Woo
• How to construct lab WWW page   Jim Woodgett
• Remove SDS from protein preparation   Chao-Liang Wu
• Q: PCR cloning from cDNA library   Jinling Xie
• ligation-mediated PCR   H YANG
• Reasons for PCR failure....?   Mayumi Yagi
• Rules of thumb for sequencing cloned PCR products?   Mayumi Yagi
• Vent polymerase from NEB   Chin-Fen Yang
• TEST   Feng Yang
• non-denaturing protein gel electrophoresis recipe?   Feng Yang
• blunt end ligation problems   HY Yang
• Detergent for Tissue Culture glassware   George Yeung
• Tricks for pipetting detergents??   H. Yim
• Tetracycline inducible system   Dong Yin
• EBV/LMP-1   Chui Yiu-Loon
• EBV/LMP-1   Chui Yiu-Loon
• No banding in CsCl Spin,Why?   Stephanie Yong
• Searching for oleosin probe   Stephanie Yong
• Smeary touchdown PCR gel.   Stephanie Yong
• "Translabel" isotopes   Ian A. York
• Somatic cell hybridization   Ian A. York
• Qiagen: This is ridiculous   Ian A. York
• Qiagen: This is ridiculous   Ian A. York
• Ultracentrifuge experiences?   Ian A. York
• DNA gel extraction   Taek H. You
• DNA gel extraction   Taek H. You
• RNA PURIFICATION   Taek H. You
• Northern Normalization   Taek H. You
• RT-PCR difficulty - PLEASE HELP!!!!!   Taek H. You
• Help with Genomic PCR   Taek H. You
• First Base, free molecular biology software exclusively for OS/2   Taek H. You
• LiCl in DNA prep   Taek H. You
• Sequencing directly from gel slices ?   Taek H. You
• RT-PCR difficulty - PLEASE HELP!!!!!   Taek H. You
• PKC assay in Hela cells   David Tak Yan Yu
• No banding in CsCl Spin,Why?   Zhiyong Zhao
• RNA Purification   Zhiyong Zhao
• problems with gel mobility shift assay   Zhang Zhiying
• Which company sell Maxam-Gilbert reaction kit?   Fanxiu Zhu
• DNA don't migrate...   Guan Zhu
• Thrombin Cleavage of GST   Guan Zhu
• whole mount in situ hybridization   a101anne at horus.sara.nl
• transcription termination   ae at dna.bio.warwick.ac.uk
• expressed protein is 5-6kda bigger than   ae at dna.bio.warwick.ac.uk
• Reasons for PCR failure....?   jason affourtit
• DNA dye for fluorometric quantitation   alex at alfred.med.monash.edu.au
• AmpliTaq Gold vs AmpliTaq: where is the magic ?   bb1dopeg
• AmpliTaq Gold vs AmpliTaq: where is the magic ?   bb1dopeg
• AmpliTaq Gold vs AmpliTaq: where is the magic ?   bb1dopeg
• yeast expression vector   bhalerao
• size of pBI 121?   dinakar bhattramakki
• DNA dye for fluorometric quantitation   bigcheez at ma.ultranet.com
• Lambda ZAP as a vector for bacterial libraries   billing at vetsci.microvet.arizona.edu
• expressed protein is 5-6kda bigger than   billing at vetsci.microvet.arizona.edu
• Cleaning Quartz Cuvettes   billing at vetsci.microvet.arizona.edu
• Carbos. in DNA prep!!!!!!   billing at vetsci.microvet.arizona.edu
• Temperature Sensitive pACYC or Derivatives   billing at vetsci.microvet.arizona.edu
• U937 cell extracts, retardation assay   bodammer at mailer.uni-marburg.de
• Affordable Patent Searching   breffni at neca.com
• SDS PAGE band blurring   brett
• No banding in CsCl Spin,Why?   brett
• Tricks for pipetting detergents??   brett
• Seamless Cloning->LIC (ligation independent cloning)   brett
• Eukaryotic protein expression   brett
• Qiagen: This is ridiculous   brett
• Concentrating proteins already in SDS sample buffer?   brett
• Bpu10I enzyme   carlo at florina.angers.inra.fr
• transgenic mice   carreer
• transgenic mice   carreer
• I wish radioactive colloidal chitin!!   choslab
• CFTR mutation analysis   Mike Morris sur cmusun12
• Spotty microsatellite sequencing gels:HELP (was: bind silane and sequencing)   craig.primmer at bmc.uu.se
• How many Kb in a centiMorgan????Help   dasuser at PO-Box.McGill.CA
• Using TdT to clean up sequencing reactions?   drdad at ktc.com
• Using TdT to clean up sequencing reactions?   drdad at ktc.com
• subscription   susan duthie
• Using TdT to clean up sequencing reactions?   edwardm at hhmi.ucla.edu
• Cleaning Quartz Cuvettes   edwardm at hhmi.ucla.edu
• lysis of RAW macrophage-like cell line   eheinecke at STUDENTS.WISC.EDU
• DNA gel extraction   ehwang at ucdavis.edu
• (none)   fotgar!fotgar.uba.ar!metodos at ccc.uba.ar
• Qiagen Quiaquick : reliable? pure enough for Autoseq?   futers at biovax.leeds.ac.uk
• Protocol for Drosphila genomic DNA prep   gc
• pBEND anyone?   gc
• Dyestuff-biotech stain   gem at pcnet.com
• Probes for rat ISH   kevin gilbride
• Postdoc position in New Zealand available   hraapg at marccri.marc.cri.nz
• PCR - SSCP loading mixture/denaturant??   Administrador de iihema.fmed.uba.ar
• GST-fusions and proteolysis   ijiwaru at wheel.dcn.davis.ca.us
• pGEX/GST gene fusion problems-> HELP!   ijiwaru at wheel.dcn.davis.ca.us
• Eukaryotic protein expression   ijiwaru at wheel.dcn.davis.ca.us
• Ligating small insert   ijiwaru at wheel.dcn.davis.ca.us
• RNA extraction   Farid jalali
• anti-heparin and EDTA PCR tricks   jbw.hammond at ukonline.co.uk
• does IPTG go bad???   jfh
• IEC micromax   jfh
• renaturing proteins   jgluz at STUD.MED.CORNELL.EDU
• Vector for direct cloning of PCR products   jlight at SICKKIDS.ON.CA
• Eukaryotic protein expression   jstrassw at OPAL.TUFTS.EDU
• test   jwobrien
• Glycogen for co-precipitating DNA   jwobrien
• smear Northern   kankaku
• HELP HELP HELP needed with RNA extraction   alex emil kentsis
• Shearing genomic DNA   Majumder lab
• Sections not sticking to slides   Majumder lab
• expressed protein is 5-6kda bigger than   lmcmurry_tet at opal.tufts.edu
• normalizing expression   lmrc.
• Nucleotide Sequence of pTich5   takashi matsumoto
• How determine target sequence of a novel DNA binding protein   mcatania at ucla.edu
• Beckman system gold nouveau   hassane mchaourab
• hemocyte marker?   mhlee
• How do I terminate transcription at a specific site in a plasmid that transfected into mammalian cells???   michael.baron at bbsrc.ac.uk
• Oligo-services...   milkman
• Transgenic reporter constructs   milstone
• Harris -80 Freezers   mpk6
• Sequence homology shareware   mpoulin at s-and-s.com
• who makes 'OLIGO' software? - forget them! go with DNASTAR!   mullenlynne at jsei.ucla.edu
• CAT   susanne müller
• sexing birds   nigel at mendel.berkeley.edu
• Harris -80 Freezers   nigel at mendel.berkeley.edu
• Searching desperately for pJM17 (E1 adenovirus)   noone
• DIG Northerns   nsaunders at molbiol.ox.ac.uk
• PCR - SSCP loading mixture/denaturant??   alex parker
• Direct Protection Assay Help...   john phelan
• DCOOD anhydrous: IS IT A DREAM?   piero pollesello
• BTX electroporator   johanna preiszner
• ISO: Next Plant Genome Meeting   johanna preiszner
• DNA Gel extraction   pudwell at qut.edu.au
• 2-hybrid library for Candida albicans?   john w. puziss
• size of pBI 121?   pzhang
• antibiotics for LBA4404   pzhang
• ref Quantitative PCR   raconte at ibm.net
• LiCl in DNA prep   rcowherd at mail.med.upenn.edu
• DNA don't migrate...   rcowherd at mail.med.upenn.edu
• Green Balancer ?   reichhart at astorg.u-strasbg.fr
• Green (GFP) Balancer (bis) ?   reichhart at astorg.u-strasbg.fr
• CAT gene   albano renee
• ssDNA->dsDNA transfos...was:annealing of ssDNA...   rhubner at molbiol.ox.ac.uk
• First Base, free molecular biology software exclusively for OS/2   rhughes at ucsd.edu
• First Base, free molecular biology software exclusively for OS/2   rhughes at ucsd.edu
• Staining gels in a microwave   rjanssen_imm at opal.tufts.edu
• about transformation of DNA into E.Coli   jane sandall
• chloramphenicol   p.j. van santbrink
• 3' prime radiolabeling of oligo using dideoxynucleotides   p.j. van santbrink
• Molecular biology software search   sczpjh at szn1.nott.ac.uk
• Qiagen: This is ridiculous   sharmaa at acsu.buffalo.edu
• Test. Pls. ignore   sharmaa at acsu.buffalo.edu
• Hybond N Problems   sunil shaw
• dsRNA Killer Virus M probe   sherif at DARWIN.UCSC.EDU
• T-vector   ssd2rc at po.iijnet.or.jp
• Quantitation of individual nucleotides   stein.bergan at labmed.uio.no
• looking for a method to strip gel plates   brad swanson
• Looking for different CAT assay protocols !   th.neumann at dkfz-heidelberg.de
• DNA electrophoresis   u.381 at inserm.u-strasbg.fr
• <None>   varyc.mmcwp4 at mmc.org
• gradient robocycler help   wegloehner at mpimg-berlin-dahlem.mpg.de
• PCR and food colors/dyes   wick at cris.com
• Please Help (pMAL)   wsa
• NIH-Image   Hongyu yang
• NIH-Image   Hongyu yang
• RAPD Trouble!!!   Hongyu yang
• Help: genomic DNA purification   Hongyu yang
• Need help with CAT assays in bacteria   yugtuyg
• who makes 'OLIGO' software? - forget them! go with DNASTAR!   zinc
• gradient robocycler help   zinc
• How to establish a stable pure line from transgenic plant?   §Ñ¼~¯ó
• Better method for deglycosylation...?   Àå°ÇÈñ

Last message date: Sat Aug 31 15:12:12 EST 1996
Archived on: Sun Mar 6 21:10:22 EST 2005

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