Methods August 1996 Archives by author
Starting: Thu Aug 1 00:34:52 EST 1996
Ending: Sat Aug 31 15:12:12 EST 1996
Messages: 1169
- who makes 'OLIGO' software? - forget them! go with DNASTAR!
"Stephen A. Bustin 3123", SURG
- reply: SSCP: Restriction Enzymes vs. Shorter Fragments
Gregory S. Buzard 'Buz'
- Reasons for PCR failure....?
Gregory S. Buzard 'Buz'
- PCR-SSCP loading mixture/denaturant??
Gregory S. Buzard 'Buz'
- Removal of Ethidium Bromide
Ed Seijo (BIO)
- Sequencing PCR product by degenerateprimer
*
- NH4Ac-Miniprep becomes bad - why?
*
- Patent on GeneClean?
*
- Patent on GeneClean?
*
- NH4Ac-Miniprep: The Protocol
*
- MICROEXTRACTION.
- E. coli host strain for transformation
.69g
- Reasons for PCR failure....?
:-Peter
- E. coli host strain for transformation
?=4~$^
- RNA PURIFICATION
MCCABE AJ
- RNA Purification
MCCABE AJ
- inducible mammalian promoter
NETSCAPE APPLICATION
- inducible mammalian promoter
NETSCAPE APPLICATION
- Antibodies to 6-His Tag
AShaw5
- expressed protein is 5-6kda bigger than
AShaw5
- Microcentrifuge preferences???
AShaw5
- Thrombin Cleavage of GST
AShaw5
- pGex expression
AShaw5
- Zoo blots
Mr Hassan Abdulrazzak
- Second hand zoo blots
Mr Hassan Abdulrazzak
- dhfr activity assay?
Shanthi Adimoolam
- BIOSCI/bionet miniFAQ & Fundraiser
BIOSCI Administrator
- blunt end ligation problems
Jeremy C. Ahouse
- puromycin selection in e. coli and lymphoblastoid cell lines (LCLs)
Ashok Aiyar
- Antibodies to 6-His Tag
Ashok Aiyar
- Cloning PCR products
Ashok Aiyar
- re-using electroporation cuvettes
Ashok Aiyar
- Selection with puromycin
Ashok Aiyar
- Selection with puromycin
Ashok Aiyar
- pBEND anyone?
Ashok Aiyar
- Help with DD RT-PCR??
Louis Alarcon
- myoglobin cDNA
Louis Alarcon
- Wanted: Mouse B-cell library
Shane Albright
- Gene knockout in E.coli
P.R. Alefounder
- Taq DNA ligase and PCR
P.R. Alefounder
- Taq data concealed?(was: Re-PCR not allowed)
Bill Alexander
- Removing endotoxin from plasmids
Mikhail Alexeyev
- size of pBI 121?
Mikhail Alexeyev
- Specificity of NEM toward cysteine residues
Mikhail Alexeyev
- Invitrogen's pZERO & E.coli with ccdA
Mikhail Alexeyev
- Invitrogen's pZERO & E.coli with ccdA
Mikhail Alexeyev
- Gene knockout in E.coli
Mikhail Alexeyev
- Need help with CAT assays in bacteria
Mikhail Alexeyev
- HELP!!! Lambda prep...
Allison
- Eliminate light/heavy chain after IP??
Danny Altschuler
- Selection with puromycin
Danny Altschuler
- GST fusions forming dimers - reference, PLEASE
Chen Ho An
- long PCR primer
Chen Ho An
- I need a PROTOCOL !!!!
Victor Anaya-Baez
- Looking for antibodies against Cre-recombinase
Thomas R. Anderson
- Antibodies to cre recombinase
Thomas R. Anderson
- anti-heparin and EDTA PCR tricks
Dan Andrews
- Biotin Pcr primers and anealing temperature
Dan Andrews
- Help:ss-DNA
Anja
- NIH Image advice...
Dr Alexander J Annala
- BioRad CHEF DR-II Pulsed Field Gel Electrophoresis Apparatus
Dr. Alexander J. Annala
- source of annexin
Joanna Aretz
- glycerol to avoid protein precipitation?...
Arioch
- Chloropane ??? what the h... ???
Josee Ash
- NH4Ac-Miniprep: The Protocol
Josee Ash
- E.coli cultures lysing after induction
Peter Ashton
- Position Open
Technical Assistance
- FITC fading kinetics
Graham Atherton
- DNA gel extraction
W R BENNETT
- Remove SDS from protein preparation
W R BENNETT
- GST-fusions and proteolysis
BIOTEC54
- Plasmid amplification in Cm-r E.coli
DAVE BOYD
- Help-Lg-scale Bacterial Genomic DNA Prep
Joel Bader
- Carbos. in DNA prep!!!!!!
Joel Bader
- CAT gene
Tim Barnett
- DNA Quantitation-Quick and Dirty
David Barrass
- cloning in M13
Kirk A Bartholomew
- cleavage of MBP fusion proteins
Guriq Basi
- subcloning pcr bands
L. Bastian
- Reasons for PCR failure....?
Jim Beasley
- A problem in topology
Ed Beaty
- DIG-labelled RNA probes
Ed Beaty
- CAT gene -- fluorescent substrates?
Ed Beaty
- UV safe TAE ?
Ed Beaty
- biotinylated nucleotide source?
Ed Beaty
- DNA-quantification by software
Andreas Becker
- Primer Design???
Andreas Becker
- Taq polymerase
Robin Beech
- PCR detection of HLA alleles
Robin Beech
- info on polyclonal antibodies against soybean BBI needed
Vadim Beilinson
- Re- Smeary Touchdown PCR
Brett Beitzel
- Re- PCR not allowed
Brett Beitzel
- Re- Amplitaq Gold
Brett Beitzel
- Re- Cloning PCR Products
Brett Beitzel
- Cheap Eporation Cuvettes?
Bob Belas
- trehalose determination
Ed Bellion
- Temperature Sensitive pACYC or Derivatives
Michael Benedik
- freeze-squeeze (Re: geneclean: vortexing)
Trevor Bezdek
- about transformation of DNA into E.Coli
Trevor Bezdek
- source for ICE or IchL
Trevor Bezdek
- Searching desperately for pJM17 (E1 adenovirus)
Trevor Bezdek
- Taq polymerase patent (not)
Tom Bickle
- vaccination with HIS tag protein
Stephen J Billington
- Cosmids
Stephen J Billington
- BioRad PROTEIN A BINDING BUFFER
Jeffrey Bjorge
- Harris -80 Freezers
Bruce Thomas Blakely
- Director Level Position
Martin Blankfard
- Cloning PCR products
Volker Blaschke
- Hela Cells and Adenylylcylase Components
Tracy Blevins
- Gram-positive sequence similarities-info req
Maria Bobrowska
- DNA electrophoresis
Maria Bobrowska
- B.napus genomic library
Peta C. Bonham-Smith
- Poly A Northern normalisation
Peta C. Bonham-Smith
- Annealing of two complementary circular SS DNAs
Joe Boutell
- Help me! / Yeast transformation in Two-Hybrid screening
Joe Boutell
- DNA contanimate
Joe Boutell
- Chloropane ??? what the h... ???
Robot Boy
- Annealing of two complementary circular SS DNAs
Chris Boyd
- Trizol
Susan Boyle-Vavra
- Help with PCR library screening
John Brennand
- Cloning vector with kanamycin instead of ampicillin resistance
John Brennand
- Size fractionation of DNA
John Brennand
- How do I terminate transcription at a specific site in a plasmid that transfected into mammalian cells???
Andrew Brooks
- HELP HELP HELP needed with RNA extraction
Paul A Bucciaglia
- How to "normalize"
Buddha
- Primer Design???
Buddha
- Thrombin Cleavage of GST
Evan Burkala
- Reasons for PCR failure....?
Bill Burnett
- Oligo-services...
Tonja Burshek
- RT-PCR difficulty - PLEASE HELP!!!!!
Tonja Burshek
- RT-PCR difficulty - PLEASE HELP!!!!!
Tonja Burshek
- catalase/tissue culture
Tim Buss
- Large MW Standard f. SDSPAGE
Dr E. Buxbaum
- Fiske and Subbarow reagent . . .
Dr E. Buxbaum
- Microcentrifuge preferences???
Dr E. Buxbaum
- Formaldehyde aliquots
Dr E. Buxbaum
- Tricks for pipetting detergents??
Dr E. Buxbaum
- glycerol to avoid protein precipitation?...
Dr E. Buxbaum
- Cleaning Quartz Cuvettes
Dr E. Buxbaum
- test: please, ignore me !
Christine CARTIER
- Custom BAC libraries?
STACY A CIUFO
- geneclean: vortexing
Manuel G. CLAROS
- HELP HELP HELP needed with RNA extraction
Manuel G. CLAROS
- How to denature target DNA on nylon membrane?
CRH
- Help with Genomic PCR
Miguel Cabrita
- Elongase vs. Expand: Which is better?
Miguel Cabrita
- ISO: Next Plant Genome Meeting
Roy G. Cantrell
- Where is the gfp group
Roberto Cappai
- endotoxin assay kit
Leslie Carvalho
- VVND
Leslie Carvalho
- Plasmid pKK3535?
Michael Casey
- BIOMETRA - Thermoblock ?
Michael Casey
- Where is the gfp group
Warren Casey
- Where is the gfp group
Warren Casey
- Statistics and biology
Edmundo Castro
- Glycogen for co-precipitating DNA
Edmundo Castro
- AMBIONS ANTISENSE 18S TEMPLATE
Jennifer L Causey
- AMBIONS ANTISENSE 18S TEMPLATE
Jennifer L Causey
- AMBIONS ANTISENSE 18S TEMPLATE
Jennifer L Causey
- Test - no message
Mike Challen
- Cy5 Custom DNA Synthesis
Dr Alan Chan
- Cy5 Custom DNA Synthesis
Dr Alan Chan
- Cy5 Custom DNA Synthesis
Dr Alan Chan
- BioRad PROTEIN A BINDING BUFFER
Alex Chang
- postivie control of HIV-1 Amplicor
Alex Chang
- How to denature target DNA on nylon membrane?
Jeremy Chen
- promoter screening
Leung Kwan Chi
- PCR primer selection software
LAU Tze Chin
- Carbos. in DNA prep!!!!!!
Tim Chipman
- Qiagen: This is ridiculous
Nikolai Chitaev
- Selection with puromycin
Nikolai Chitaev
- a good protocol for far westerns/overlays??
Nikolai Chitaev
- Your basic stupid question about PCR cloning
Dr. Duncan Clark
- Bind-Silane and sequencing
Dr. Duncan Clark
- Wedge Sequencing gels
Dr. Duncan Clark
- DNA size markers
Dr. Duncan Clark
- Mung bean nuclease ?
David Clarke
- postivie control of HIV-1 Amplicor
Dr. J.P. Clewley
- postivie control of HIV-1 Amplicor
Dr. J.P. Clewley
- pGEX/GST gene fusion problems-> HELP!
Steve Cohen
- Qiagen refuses to tell!
Steven Cohen
- Qiagen: This is ridiculous
Steven Cohen
- test
Allan Colthart
- RNA Quantification...
Allan Colthart
- Nylon and Silver staining
Pierre Commercon
- Red Cell Band 4.1
Dr. Brian M. Cooke
- green fluorescent protein
Michael Cooley
- Tricks for pipetting detergents
Dan Coovert
- isolation of DNA from mouse tails
Seth Corey
- Yeast contamination on cell culture:HELP!!
Joao Vasconcelos Costa
- Antibodies to poly-His
Maria C. Costanzo
- long PCR kits
Michael B. Coulthart
- Southern Blotting of Pulse Field Gels.... HELP!!?
Pascal Cousin
- Differential display kits - recommendation please
Vernon Coyne
- DOW CHEMICAL JOINS GLOBAL CONSORTIUM ON ELECTRONIC LAB NOTEBOOKS
CreateENBs at aol.com
- DOW CHEMICAL JOINS GLOBAL CONSORTIUM ON ELECTRONIC LAB NOTEBOOKS
CreateENBs at aol.com
- Specificity of NEM toward cysteine residues
Dominique DELFORGE
- How to "normalize"
DREDD
- Primer Design software Homepage
DREDD
- Your basic stupid question about PCR cloning
DREDD
- Microcentrifuge preferences???
DSF
- Tricks for pipetting detergents??
DSF
- DNA electrophoresis
R Daines
- Qiagen refuses to tell!
Richard Daines
- Problems with ligation after GeneCleaning
Nico Dantuma
- (none)
Martin Daoust
- (none)
Martin Daoust
- Indicators for electroporation eficiency
Levan Darjania
- 4+2 rule
Robert Darnell
- "Translabel" isotopes
David L. Haviland, Ph.D.
- Oligo-services...
David L. Haviland, Ph.D.
- PCR Not Allowed
David L. Haviland, Ph.D.
- hydropathy
Oliver S. DePeyer
- Qiagen: This is ridiculous
Oliver S. DePeyer
- Qiagen: This is ridiculous
Oliver S. DePeyer
- Reasons for PCR failure....?
Tom Deits
- How many kb in a centiMorgan??
Graham Dellaire
- New at Experimental Medicine Job Listings!!!
Graham Dellaire
- Southern Blotting of Pulse Field Gels.... HELP!!?
Graham Dellaire
- Sliver staining DNA
Jacqueline Delp
- RNA-extraction from PAGE
Detlef.Ritter at stud.uni-hannover.de
- pGEX/GST gene fusion problems-> HELP!
Michael DiDonato
- pGEX/GST gene fusion problems-> HELP!
Michael DiDonato
- Smeary touchdown PCR gel.
Dietmar
- Continuous gel ref
Paul Digard
- Factor Xa Cleavage
Paul Digard
- Help with nuclease S1 protocol
Dinakar
- chloramphenicol
John Dixon
- RT-PCR difficulty - PLEASE HELP!!!!!
John Dixon
- what conc antibiotics for Carb/Kan/Tet cocktail?
John Dixon
- Biotin Pcr primers and anealing temperature
John Dixon
- Re- Cloning PCR Products
Andrew J. Doherty
- Cloning PCR products
Dr Andrew J. Doherty
- RNA Quantification...
Colin Dolphin
- more Hybond-N problems
Gerald B. Downes
- Genetrapper kit
Dr N.J. Doyle
- catalase/tissue culture
Harold Drabkin
- MluI cuts like it wants
Richard J. Dudley
- HELP!!! Fungal contamination of IMPORTANT transfectants!
Richard J. Dudley
- Yeast contamination on cell culture:HELP!!
Richard J. Dudley
- Where to buy pentosidine?
KC Dunn
- Northern Normalization
Ken Dwyer
- fungal genomic DNA
EGUARINOS at MVAX.CBM.UAM.ES
- UV crosslinking of DNA to proteins
Simon Easterbrook-Smith
- who makes 'OLIGO' software? - forget them! go with DNASTAR!
Rubin Eitan
- Absorbance spectrum for Xgal product
Michael B. Elowitz
- Grant Available - Genetic Engineering - Fungi
Mark Emalfarb
- Active Component in Skunk Urine
Dianne Emslie
- SYBR Green II RNA coloration
Isabelle Ernest
- pGEX/GST gene fusion problems-> HELP!
Mark Evans
- Evaluating reverse transcription
Patrick HJ Falckh
- RNA Centrifuge Spins
Patrick HJ Falckh
- Harris -80 Freezers
Mark Farinha
- Size fractionation of DNA
Michael Feldman
- looking for a method to strip gel plates
Karl Fischer
- Tricks for pipetting detergents??
Robert Fjellstrom
- Uncouplers & pH
L. Fliegel
- Sequencing directly from gel slices ?
Bryan L. Ford
- Active Component in Skunk Urine
Gerald Forrest
- Total RNA labelling
G.W.Phillips
- Specificity of NEM toward cysteine residues
Michael Galperin
- N-terminal His-tag expression vector
Susana Galvez
- Chloropane ??? what the h... ???
Olivier Gandrillon
- Loss of polymorphism
Pooba Ganeshan
- Comparing products Magazine
Frederick Garbrecht
- Your basic stupid question about PCR cloning
Frederick Garbrecht
- PCR variability with DNA extraction method
Frederick Garbrecht
- DNA gel extraction
Frederick Garbrecht
- Liposomal transfection into human hematopoetic stem cells
Frederick Garbrecht
- Permenant mounting of AP-NBT/BCIP slides
Wes Garrett
- standard for northern blot analysis
Dr. Benoit Gauthier
- RNA sizing against DNA markers
Dr. Peter Gegenheimer
- Toxic fragment in pBR322
Stephan Geley
- What is: the toxic fragment in pBR322?
Stephan Geley
- Discrepancy between OD-value on spec and DNA on agarose gel
Louis Geller
- Problems with ligation after GeneCleaning
Gabby Gerlitz
- DNA contanimate
Gabby Gerlitz
- Help me! / Yeast transformation in Two-Hybrid screening
Sourav Ghosh
- bioluminescence-coelentrazine
Luc Giasson
- Carbenicillin
Torben Gjetting
- Help: genomic DNA purification
Michelle Gleeson
- DNA gel extraction
Michelle Gleeson
- removing fungal contamination from cell cultures
Michelle Gleeson
- LambdaGEM 11 Xho gLibrary
Michelle Gleeson
- looking for a method to strip gel plates
Michelle Gleeson
- Does Taq Stoffel fragment produce 3' A overhangs.
Michelle Gleeson
- NH4Ac-Miniprep becomes bad - why?
Michelle Gleeson
- software for DNA and Protein comparision
Jack Goff
- UV mutagenesis
Steven Goldberg
- Problems with ligation after GeneCleaning
Steven Goldberg
- pGEX/GST gene fusion problems-> HELP!
Mark Gosink
- Vector for direct cloning of PCR products
Dr Ian Graham
- Chlorampehical inhibit ssDNA packaging?
Dr. James E. Graham
- Using ClaI for subcloning
Dr. James E. Graham
- Taq polymerase patent (not)
Dr. James E. Graham
- sexing birds
Dr. James E. Graham
- freeze-squeeze (Re: geneclean: vortexing)
Jim Graham
- lysis of RAW macrophage-like cell line
Jim Graham
- Enumerating adherent cells (w/o harvest)?
Jim Graham
- Taq polymerase
Jim Graham
- Disinfectant contact t & Mycobacterium tuberculosis
Jim Graham
- Annealing of two complementary circular SS DNAs
Lloyd Graham
- antibody against human L1
L. Griffith
- Freeze Shearing of DNA
Steve Griffiths
- AmpliTaq Gold vs AmpliTaq: where is the magic ?
Steve Griffiths
- Shearing genomic DNA
Keith Grimaldi
- geneclean: vortexing
Charles M Griswold
- PCR Not Allowed
Duke Groebe
- Biotin Pcr primers and anealing temperature
Duke Groebe
- Hybond N Problems
Mike Gruidl
- Hybond N Problems
Mike Gruidl
- DNA contanimate
Halil-Cem Guersoy
- Anyone tried Sypro Gel Stains???
Toumy Guettouche
- glycerol to avoid protein precipitation?...
Toumy Guettouche
- NH4Ac-Miniprep becomes bad - why?
Toumy Guettouche
- RPA, protect bigger band than probe
Xin Guo
- poly-A purification
Szabadkai Gyorgy
- Help with DD RT-PCR??
Alex Haddad
- Taq polymerase
Alastair Hamilton
- Reasons for PCR failure....?
Gerald Hamilton
- Help me! / Yeast transformation in Two-Hybrid screening
Michael Hamilton
- 10 %FCS
Clemens Oliver Hanemann
- pGEX/GST gene fusion problems-> HELP!
Brandon Harder
- a good protocol for far westerns/overlays??
Gerald W. Hart
- Source for 3'-dCTP???
Alex Harvey
- M1/70 Ab and Western Blots
'Fumi' Fumitaka Hayashi
- food colors in PCR
Paul N Hengen
- about transformation of DNA into E.Coli
Paul N Hengen
- Pointer to FAQ lists
Paul N Hengen
- Shearing genomic DNA
Paul N Hengen
- about transformation of DNA into E.Coli
Paul N Hengen
- Sliver staining DNA
Paul N Hengen
- Screening Libraries by PCR
Paul N Hengen
- about transformation of DNA into E.Coli
Paul N Hengen
- about transformation of DNA into E.Coli
Paul N Hengen
- Hybond N Problems
Paul N Hengen
- Glycogen for co-precipitating DNA
Paul N Hengen
- expressed protein is 5-6kda bigger than
Paul N Hengen
- DNA gel extraction
Paul N Hengen
- expressed protein is 5-6kda bigger than
Paul N Hengen
- Church buffers (Was Re: Hybond N)
Paul N Hengen
- pGEX/GST gene fusion problems-> HELP!
Paul N Hengen
- EMBO/ICGEB Phage Display Course
Paul N Hengen
- food colors in PCR
Paul N. Hengen
- Antibody to CKR5 ?
Mike Herron
- Recomend Fluor. Plate reader?
Mike Herron
- antibody to CKR5?
Mike Herron
- Has anyone separated proteins using agarose.
John Hind
- Westerns-blotting-BSA
Robert Hindges
- LambdaGEM 11 Xho gLibrary
Carsten Hoege
- MutS gel mobility shift
Les Hoffman
- UV Safe TAE
Les Hoffman
- Separating proteins on agarose
Rosemary T. Hoffman
- Reasons for PCR failure....?
Susan Jane Hogarth
- Reasons for PCR failure....?
Susan Jane Hogarth
- Qiagen: This is ridiculous
Susan Jane Hogarth
- Anyone tried Sypro Gel Stains???
Mike Holbrook
- Insect cell electroporation
Mike Holbrook
- Spotty microsatellite sequencing gels:HELP (was: bind silane and sequencing)
Paul Hollenbach
- Continuous gel ref
Louis Hom
- Uncouplers & pH
Louis Hom
- peptide/antibody production companies
Guido Hooiveld
- endotoxin assay kit
Jan Hoolwerf
- RT primer for quantitative PCR
Kathleen Hopp
- GST-fusion expression problem
Chris Houchens
- Problems with ligation after GeneCleaning
Chris Houchens
- Annealing of two complementary circular SS DNAs
Ken Howe
- Seamless Cloning
Carolyn Hoyal-Wrightson
- Thermostable DNA polymerase can be used in Taq DNA ligase
buffer?
Mr. Chris Hoyle
- expressed protein is 5-6kda bigger than
Zheng Huanquan
- expressed protein is 5-6kda bigger than
Zheng Huanquan
- Bacterially Expressed Proteins: IPTG induction
Zheng Huanquan
- Qiagen Quiaquick : reliable? pure enough for Autoseq?
Matt Hudson
- antibiotics for LBA4404
Matt Hudson
- Westerns-blotting-BSA
Matt Hudson
- Oligonucleotide purification
Lynn Hughes
- Oligonucleotide purification
Lynn Hughes
- S-35 DNA and differential display
Gene Huh
- CAT gene -- fluorescent substrates?
Gene Huh
- looking for a method to strip gel plates
Gene Huh
- E.coli cultures lysing after induction
Hong Hung
- Discrepancy between OD-value on spec and DNA on agarose gel
Jim Hutchins
- Bind-Silane and sequencing
Jim Hutchins
- Has anyone separated proteins using agarose.
Jim Hutchins
- about thermocycling sequencing
Jin-seon Im
- about squeez-freeze
Jin-seon Im
- about Tag polymerase
Jin-seon Im
- about transformation of DNA into E.Coli
Jin-seon Im
- about competent cell
Jin-seon Im
- renaturating protein
Jin-seon Im
- some polymerase better than Tag?
Jin-seon Im
- bacterially expressed protein
Jin-seon Im
- blunt end cloning
Jin-seon Im
- Second hand zoo blots
Imgenfm
- Double stranded dna Sequencing help
Immu-I
- DNA extraction from BAC clone
Hiroshi Inoue
- Problem with mtDNA-Degradation
Frederik Intelmann
- Glycogen for co-precipitating DNA
Frederik Intelmann
- HELP!!! Lambda prep...
Atsushi Isoai
- Plasmid amplification in Cm-r E.coli
Masayoshi Itoh
- MICROEXTRACTION.
Fraser Dr N J
- MICROEXTRACTION.
Fraser Dr N J
- (none)
Fraser Dr N J
- RNA extraction
Fraser Dr N J
- (none)
VASANTHARAJAN JANAKIRAMAN
- (none)
Thomas J. Jacks
- Biotin Pcr primers and anealing temperature
Timothy R Jackson
- Oligonucleotide purification
Daniel Jacobs
- Looking for H. Bujard's Tet system plasmids: pBI-4 ...
Fred Jacobs
- Primer Design???
Fred Jacobs
- Some polymerase better for PCR?
Nick Jacobsen
- Southern Blotting of Pulse Field Gels.... HELP!!?
Nick Jacobsen
- Genetic Analysis
Linda St. James
- "Biotech Rumor Mill" additions
Linda St. James
- Does Taq Stoffel fragment produce 3' A overhangs.
Jamie
- U.S.E. mutagenesis
Jane.Wyatt.1 at massey.ac.nz
- Qiagen: This is ridiculous/purity
Richard A. Janssen
- Looking for different CAT assay protocols !
Richard A. Janssen
- Need Pyrimidine Dimer-DNA Glycosylase
Scott Jelinsky
- Qiagen DNA purification resin - what is is?
Glenn Jenkins
- Tricks for pipetting detergents??
Glenn Jenkins
- Has anyone separated proteins using agarose.
Glenn Jenkins
- HELP!!! Fungal contamination of IMPORTANT transfectants!
Glenn Jenkins
- MCH in mammals
Anabela Pinto Jensen
- Active Component in Skunk Urine
Dan Johnson
- MOLCULAR WEIGHT MARKERS
Doug Johnson
- DNA size markers
Dr. Douglas A. Johnson
- Church buffers (Was Re: Hybond N)
Mark Jordan
- DNA contanimate
Birgit Jostes
- Pipette for PCR
Birgit Jostes
- Mung bean nuclease ?
Fourie Joubert
- re-using electroporation cuvettes
Justinian
- Cy5 Custom DNA Synthesis
K.K.Chan
- Test
K.K.Chan
- Test
K.K.Chan
- Cy5 Primers
K.K.Chan
- Cy5 Primers
K.K.Chan
- normalizing expression
K.Kramer
- AMBIONS ANTISENSE 18S TEMPLATE
K.Kramer
- sexing birds
GERALDINE KAMINSKI
- Roche AMPLICOR HCV PCR
GERALDINE KAMINSKI
- 5 min transformation E. coli
KHUANG at CHEMVX.TAMU.EDU
- 5 min transformation E. coli
KHUANG at CHEMVX.TAMU.EDU
- How to construct lab WWW page
KHUANG at CHEMVX.TAMU.EDU
- Sequencing PCR product by degenerateprimer
KHUANG at CHEMVX.TAMU.EDU
- Sliver staining DNA
D. KIM
- RNA sizing against DNA markers
D. KIM
- Mutagenesis: Does QuickChange (TM) work well?
Stefan Kahlert
- Need Mac NIH Grant Forms
Alan Kaiser
- Qiagen buffer contents
Fredrik Kamme
- Qiagen refuses to tell!
Fredrik Kamme
- Yeast cDNA bank in yeast vector?
Aletta Karsies
- Q. gel photo documentation systems.
Ken Kaufman
- gradient robocycler help
Jill Kellogg
- Need Help with Cos-7 Transfection and Transit
Matthew Kennedy
- Statistics and biology - a text book
Nigel Kenward
- Qiagen: This is ridiculous
Nigel Kenward
- Dig-riboprobes and RNase A
Gabriele Kerber
- Dig-riboprobes and RNase A
Gabriele Kerber
- RNA Purification
Michael Kertesz
- cDNA sequencing
Islam Khan
- Inserting cDNA into promoter constructs???
Obaid Y. Khan
- Antibody contamination during immunoaffinity purification
Dooyeon Kim
- Tricks for pipetting detergents
Sarah King
- (none)
Masato Kinoshita
- U.S.E. mutagenesis
Tim Kirksey
- GST fusions forming dimers - reference, PLEASE
Dima Klenchin
- Cellulose-substrate for Chromatography
Dima Klenchin
- Detergent for Tissue Culture glassware
Dima Klenchin
- lower detection limit with Coomssie?
Dima Klenchin
- c600
David B. Knaebel
- who makes 'OLIGO' software?
Dave Knorr
- X-gal staining without non-specific in the rat brain ?
Riekeltje Koedood
- Which company sell Maxam-Gilbert reaction kit?
Riekeltje Koedood
- What really indicates induction?
Riekeltje Koedood
- Transgenic reporter constructs
Riekeltje Koedood
- which phophoImager?
Riekeltje Koedood
- RAPD Trouble!!!
Roland Koelliker
- Possible reasons for RAPD failure
Roland Koelliker
- heme and binding
Patrick Koppitz
- Tricks for pipetting detergents??
Piotr Kozbial
- about transformation of DNA into E.Coli
Alexander Kraev
- Church buffers (Was Re: Hybond N)
Alexander Kraev
- Sequencing directly from gel slices ?
Alexander Kraev
- Fluorescent Nucleotides
Janice Kranz
- Qiagen DNA purification resin - what is it?
Cornelius Krasel
- glycerol to avoid protein precipitation?...
Cornelius Krasel
- biotin labelling
Joachim Kremerskothen
- DEHYDRATED CULTURE MEDIA $18/LB
David Kristofferson
- Help with RNA extraction method
Karen Kroeger
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Dubear Kroening
- Epitope tags: Methionines?
Arle Kruckeberg
- Primer Design???
Gary Kucera
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Gary Kucera
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Gary Kucera
- RT-PCR
Gary Kucera
- Test, Please Ignore
Gary Kucera
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Gary Kucera
- Broad-range host for E.coli phages
Peter Kuhnert
- which phophoImager?
Rork Kuick
- truncated vector
Alexander N. Kukushkin
- Identification of a c-DNA clone
Rajesh Kumar
- DNA labelling
Vivek Kumar
- purity of urea for sequencing gels
martin LEACH
- Oligonucleotide purification
martin LEACH
- How many kb in a centiMorgan??
martin LEACH
- Your basic stupid question about PCR cloning
martin LEACH
- sexing birds
martin LEACH
- How to construct lab WWW page
martin LEACH
- MedImmune INc anyone ??
martin LEACH
- gradient robocycler help
martin LEACH
- (none)
LIMARMG%BRUFMG.BITNET at UBVM.CC.BUFFALO.EDU
- UV safe TAE ?
Rico Laage
- UV safe TAE ?
Rico Laage
- Qiagen: This is ridiculous
Tsichlis Lab
- Statistics and biology
John Ladasky
- PCR detection of HLA alleles
John Ladasky
- hnRNP isolation
Michael Robert Ladomery
- hybridization of expression lib
James Warren Lafollette
- Problems with ligation after GeneCleaning
James Warren Lafollette
- insect cell fusion
Bill Langridge
- Mung bean nuclease ?
Karah Lanier
- DNA-binding protein data file?
Jean-Francois Larrivee
- DNA-binding protein question
Jean-Francois Larrivee
- Anyone tried Sypro Gel Stains???
Michael Lassner
- RNA Quantification...
Edouard Lauzier
- OD at 320nm
Edouard Lauzier
- who makes 'OLIGO' software? - forget them! go with DNASTAR!
Andy Law
- antibiotics for LBA4404
Byeong-ha Lee
- carbenicillin
Chang-Muk Lee
- Antibodies to 6-His Tag
Jongchan Lee
- X-gal staining without non-specific in the rat brain ?
Sukwon Lee
- pCMV-LacZ ?
Sukwon Lee
- test
Lee, Sun
- unsubscribe
Woo-Jai Lee
- Tet-off/Tet-on system
Philippe Lefebvre
- DNA Mass Ladder problems
Bettina Lehnhardt
- RNA PURIFICATION
Keith Leppard
- Looking for different CAT assay protocols !
Keith Leppard
- NH4Ac-Miniprep becomes bad - why?
Keith Leppard
- Remove SDS from protein preparation
Keith Leppard
- Tricks for pipetting detergents??
Wulf Dirk Leuschner
- blunt end ligation
Wulf Dirk Leuschner
- Sections not sticking to slides
Wulf Dirk Leuschner
- expressed protein is 5-6kda bigger than
Wulf Dirk Leuschner
- Molecular biology software search
Wulf Dirk Leuschner
- Other minipreps (was Re: Qiagen: This is ridiculous)
Victor Levenson
- Selection with puromycin
Victor Levenson
- NH4Ac-Miniprep: The Protocol
Victor Levenson
- Problems with ligation after GeneCleaning
Anis Limami
- Problems with ligation after GeneCleaning
Anis Limami
- INFORMATION NEEDED -Plant Antioxidants-
Anis Limami
- HELP nuclear runon nuclear runoff
Anis Limami
- Cycle sequence results?
Eric W. Linton
- peptide syntheses
Lisa Harrison-Bernard, PhD.
- Pipette for PCR
Chui Yiu Loon
- Bind-Silane and sequencing
LouPass
- Microcentrifuge preferences???
LouPass
- "Translabel" isotopes
LouPass
- Discrepancy between OD-value on spec and DNA on agarose gel
Quentin E. Low
- NIH-Image
Blanca Lupiani
- Looking for antibodies against Cre-recombinase
Jennifer Lyon
- plasmid curing
M.De-Ungria at unsw.edu.au
- DNA contanimate
M.De-Ungria at unsw.edu.au
- RT-PCR
M.MECRIN
- PCR INHIBITOR
M.MECRIN
- Antibodies to poly-His
IAN MCFARLANE
- Detergent for Tissue Culture glassware
IAN MCFARLANE
- Permenant mounting of AP-NBT/BCIP slides
Paul C. Grimm MD
- I Need Advice on single cell differential display
Paul C. Grimm MD
- Removal of Ethidium Bromide
MELANSOND
- Oligo-services...
MELANSOND
- about transformation of DNA into E.Coli
MELANSOND
- Discrepancy between OD-value on spec and DNA on agarose gel
MELANSOND
- No banding in CsCl Spin,Why?
MELANSOND
- Problems with ligation after GeneCleaning
MELANSOND
- Formaldehyde aliquots
MELANSOND
- Cleaning Quartz Cuvettes
MELANSOND
- DNA contanimate
MELANSOND
- looking for a method to strip gel plates
MELANSOND
- How to construct lab WWW page
MMCCARTHY at BIOTECHNET.COM
- Cholesterolfree FCS
FABIAN MOEBIUS
- XL-1 Red Mutagenesis
FABIAN MOEBIUS
- Flow Cytometry Protocols
John MacDougall
- Ribozyme construction
John MacDougall
- PCR variability with DNA extraction method
Dr Claudia Macaubas
- about transformation of DNA into E.Coli
Hidevaldo Bueno Machado
- blunt end ligation problems
Morris Maduro
- A problem in topology
Rafael Maldonado
- Discrepancy between OD-value on spec and DNA on agarose gel
Rafael Maldonado
- Bind-Silane and sequencing
Rafael Maldonado
- UV safe TAE ?
Rafael Maldonado
- Rules of thumb for sequencing cloned PCR products?
Bryan John Maloney
- Prodigisin Source?
Stephen W. Mamber
- Help with RNA extraction method
Ned Mantei
- Help with nuclease S1 protocol (long!)
Ned Mantei
- RPA, protect bigger band than probe
Ned Mantei
- Annealing of two complementary circular SS DNAs
Ned Mantei
- Annealing of two complementary circular SS DNAs
Ned Mantei
- Northern Normalization
Ned Mantei
- UV Safe TAE
Ned Mantei
- Double stranded dna Sequencing help
Jeremy Marcus
- Harris -80 Freezers
Jeremy Marcus
- about transformation of DNA into E.Coli
Jeremy Marcus
- Bind-Silane and sequencing
Jeremy Marcus
- No banding in CsCl Spin,Why?
Jeremy Marcus
- Problems with ligation after GeneCleaning
Jeremy Marcus
- Double stranded dna Sequencing help
Mozart de Azevedo Marins
- DNA analysis program
Mozart de Azevedo Marins
- U.S.E. mutagenesis
Mark Brandt, Ph.D.
- cleavage of MBP fusion proteins
Mark Brandt, Ph.D.
- Formaldehyde aliquots
Paula Marquardt
- Probes for rat ISH
Amanda Marsh
- Factor Xa Cleavage
G E Martin
- (none)
Martin, Martin
- Cosmids
J. Pat Martinez
- MICROEXTRACTION.
Matthew P. Frosch, M.D., Ph.D.
- pGEX/GST gene fusion problems-> HELP!
Jeanne McAdara
- Qiagen: This is ridiculous
Jeanne McAdara
- Reasons for PCR failure
Susan B. McLeskey
- CHO cells in suspension
Ruth McMahon
- cDNA visualization on agarose, HELP!!!
Gerardo Medrano
- OD at 320nm
Jose E. Mejia
- NH4Ac-Miniprep becomes bad - why?
Jose E. Mejia
- pCMVCAT
Harutyan Melkonyan
- I need a promoter selection vector, help
Merja.Mikkonen at csc.fi
- peptide/antibody production companies
Pascal Mertens
- gradient robocycler help
MichaelCataniamcatania at ucla.edu
- Contech Capfinder
Michael_J_Hansbury
- Active Component in Skunk Urine
Edward Theodore Michelini
- Bacterially Expressed Proteins: IPTG induction
Ted Michelini
- Using TdT to clean up sequencing reactions?
David Micklem
- Qiagen refuses to tell!
David Micklem
- Streck fixative for in situ
Molecular Micro
- HELP:Ab against LuECAM
Thomas Mohr
- human beta actin promoter in Euk exp. vector
Richard Moldwin
- Gene knockout in E.coli
Andy Morby
- Fugu and zebrafish DNA
D.D. Morgan
- Cy5 Custom DNA Synthesis
Dr Michael Morris
- Maxam-Gilbert sequencing ?!
Dr Michael Morris
- GENOMIC SOUTHERN: help with DNAseI Hypersensitivity (please!!!!!) (:
Heidi Moss
- Need Mac NIH Grant Forms
Scott Moye-Rowley
- Oligo-services...
Frank Murphy
- Bind-Silane and sequencing
Frank Murphy
- Qiagen: This is ridiculous
Frank Murphy
- Detecting HISx6
TJ Murphy
- Toxic fragment in pBR322
Bernard Murray
- Microcentrifuge preferences???
Bernard Murray
- Re- PCR not allowed
Bernard Murray
- AmpliTaq Gold vs AmpliTaq: where is the magic ?
Bernard Murray
- chloramphenicol
Bernard Murray
- AMBIONS ANTISENSE 18S TEMPLATE
Bernard Murray
- Antibody contamination during immunoaffinity purification
Bernard Murray
- Westerns-blotting-BSA
Bernard Murray
- Molecular biology software search
Bernard Murray
- Northern Normalization
Bernard Murray
- RNA sizing against DNA markers
Bernard Murray
- Transgenic reporter constructs
Bernard Murray
- Rules of thumb for sequencing cloned PCR products?
Bernard Murray
- lobster genome size
Michael Myers
- Cosmids
Peter J. Myler
- How many Kb in a centiMorgan????Help
Daniel Mytelka
- Help me! / Yeast transformation in Two-Hybrid screening
Takashi Nagano
- Thrombin Cleavage of GST
Lyle Najita
- test
Minh Q. Nguyen
- test
Minh Q. Nguyen
- Ligating small insert
Minh Q. Nguyen
- about squeez-freeze
Vince Nguyen
- Trypsinization and NRO
Vince Nguyen
- Nuclear run-off with DIG-UTP
Vince Nguyen
- detection of PCR products by hydridization in streptoavidin coated plates
Rick Nicholson
- What really indicates induction?
Rick Nicholson
- HELP nuclear runon nuclear runoff
Rick Nicholson
- Antibody against c-jun
Volker Noelle
- MluI cuts like it wants
Volker Noelle
- Bacterially Expressed Proteins: IPTG induction
Randy Nonay
- which phophoImager?
Randy Nonay
- 1997 FEBS Meeting on Cell Signalling Mechanisms
Albert Noorlandt
- blood spot extractions
Norm
- blood spots extractions
Norm
- oligo synthesizer - farewell!!!
Norm
- MOLCULAR WEIGHT MARKERS
Pamela Norton
- To Sailesh
Takbum Ohn
- Cre exprssing E. coli strain
Yasushi Okazaki
- pTrc99A-like plasmid...
Pierre Olivier
- PCR variability with DNA extraction method
P R Orange
- RAP PCR vs Differential display
Sandra Pena De Ortiz
- Transgenic reporter constructs
Sandra Pena De Ortiz
- Yeast contamination on cell culture:HELP!!
Stephane Ory
- Asparagine synthetase selection
Masamichi Oshima
- chloramphenicol
P.Bundock
- DIG-labelled RNA probes
GILLIAN NI BHARLO BOTANY PG
- Sections not sticking to slides
GILLIAN NI BHARLO BOTANY PG
- RNA sizing against DNA markers
GILLIAN NI BHARLO BOTANY PG
- detection of PCR products by hydridization in streptoavidin coated plates
Alejandro Palacios
- EIA detection of PCR products (streptoavidin capture)
Jose Alejandro Palacios
- A Test
Manuel Palacios
- Rules of thumb for sequencing cloned PCR products?
Mark Pallen
- DNA don't migrate...
Jukka U Palo
- SDS PAGE band blurring
Inyup Park
- Problems with ligation after GeneCleaning
Yoonsuk Park
- DNA Quantitation-Quick and Dirty
Yoonsuk Park
- Make Your Own Vacuum Pump for Gel Blotting!
Robert Passey
- Perkin-Elmer Taqman System
Bharat Patel
- Optimum conditions for Mitochondrial PCR
Tara Paton
- Northern Normalization
Susan Patrick
- What really indicates induction?
Susan Patrick
- inducible mammalian promoter
Paul_J_Ryan
- Concentrating proteins already in SDS sample buffer?
D. Pearton
- pGEX/GST gene fusion problems-> HELP!
Pete
- Kozak Sequence?
Andy Peters
- Test don't read
Rodney Earl Pettway
- test
Rodney Earl Pettway
- Carbos. in DNA prep!!!!!!
Rodney Earl Pettway
- LiCl in DNA prep
Rodney Earl Pettway
- Tricks for pipetting detergents??
Stephen P. Driska PhD
- Spots on Northern probed with oligos (50-mers)
Andy Phillips
- Where is the gfp group
Andy Phillips
- Wedge Sequencing gels
Andy Phillips
- Q: E.coli and Inositol Lipids?
C E Pical
- (none)
Venkat Pisupati
- Copy number quantitation- PCR
Daraporn Pittayakhachonwut
- E.coli cultures lysing after induction
Brent Pollock
- Eliminate light/heavy chain after IP??
Andrei Popov
- Looking for anti-CD9 and CD63 abs
Yves Poumay
- Detecting HISx6
Mark Prescott
- glycerol to avoid protein precipitation?...
Bill Prichett
- DNA extraction from BAC clone
Michael J. Prigge
- HELP!!! Fungal contamination of IMPORTANT transfectants!
Kiley R. Prilliman
- gradient robocycler help
Kiley R. Prilliman
- Yeast contamination on cell culture:HELP!!
Kiley R. Prilliman
- about transformation of DNA into E.Coli
ProZyme, Inc.
- Cloning vector with kanamycin instead of ampicillin resistance
ProZyme, Inc.
- Size fractionation of DNA
ProZyme, Inc.
- Demo DNA
ProZyme, Inc.
- Yeast contamination on cell culture:HELP!!
Xose S. Puente
- unsubscribe
REGCHAK at USTHK.UST.HK
- about transformation of DNA into E.Coli
H. ROYCHOWDHURY
- about transformation of DNA into E.Coli
H. ROYCHOWDHURY
- Discrepancy between OD-value on spec and DNA on agarose gel
H. ROYCHOWDHURY
- Bind-Silane and sequencing
H. ROYCHOWDHURY
- Re- PCR not allowed
H. ROYCHOWDHURY
- Bind-Silane and sequencing
H. ROYCHOWDHURY
- LiCl in DNA prep
H. ROYCHOWDHURY
- looking for a method to strip gel plates
H. ROYCHOWDHURY
- NH4Ac-Miniprep becomes bad - why?
H. ROYCHOWDHURY
- silver staining...
Christian Radauer
- cloning in M13
Tony Raizis
- help find peptide antisera service and information
Vineet B Rajgarhia
- DEHYDRATED CULTURE MEDIA $18/LB
Robert Randolph
- removing fungal contamination from cell cultures
Robert Randolph
- Help - problem with bFGF PCR!
Rebecca
- Cellulose-substrate for Chromatography
Achim Recktenwald
- SDS PAGE band blurring
Achim Recktenwald
- OD at 320nm
Achim Recktenwald
- glycerol to avoid protein precipitation?...
Achim Recktenwald
- glycerol to avoid protein precipitation?...
Achim Recktenwald
- Kinetics software
Achim Recktenwald
- DNA contanimate
Achim Recktenwald
- Reasons for PCR failure....?
Martha L. Reed
- Accession # for Human and Rat beta-globin gene
Mary P. Remington
- Delta RNA Fingerprinting Kit
Mary P. Remington
- Ligating small insert
S.L. Rennie
- Stains-all: Protein staining
Scott Ripley.
- lower detection limit with Coomssie?
Bruce W. Ritchings
- Antibodies to 6-His Tag
Joseph Roland
- Codons Usage software
Francisco Uribe Romeo
- sequencing
MIcha Ron
- Demo DNA
Rosnthorn at aol.com
- SV40 vs CMV promoter
Marc Rosoff
- Bacterially Expressed Proteins: IPTG induction
Jay L. Rothstein
- Microcentrifuge preferences???
Eric Roush
- I need a PROTOCOL !!!!
Rover
- Molecular biology software search
Eitan Rubin
- Does a cDNA clone contain a full length????????
Eitan Rubin
- Does a cDNA clone contain a full length????????
Eitan Rubin
- Your basic stupid question about PCR cloning
Eitan Rubin
- 4+2 rule
Eitan Rubin
- freeze-squeeze (Re: geneclean: vortexing)
Eitan Rubin
- Taq polymerase patent (not)
Eitan Rubin
- Reasons for PCR failure....?
Eitan Rubin
- RAPD Trouble!!!
Eitan Rubin
- Best prices of oligo synthesis - looking for details
Eitan Rubin
- Statistics and biology - a text book
Eitan Rubin
- purity of urea for sequencing gels
George Rutherford
- Reasons for PCR failure....?
George Rutherford
- Help with RNA extraction method
George Rutherford
- Hybond N Problems
George Rutherford
- BEMITHYL: ITS MECHANISM AND ITS EFFECTS
STEMC
- smear Northern
Klaus Salger
- Cloning vector with kanamycin instead of ampicillin resistance
Klaus Salger
- enolase
Kamran Salim
- Need help with CAT assays in bacteria
Sarah K. Highlander, Ph.D.
- Northern Normalization
Heinz-Juergen Schaefers
- Yeast contamination on cell culture:HELP!!
Heinz-Juergen Schaefers
- Problems with lipids
Wolfgang Schechinger
- European Radiochemical suppliers
Wolfgang Schechinger
- Q: Looking for GFP-cDNA
Wolfgang Schechinger
- Q: E.coli and Inositol Lipids?
Wolfgang Schechinger
- Where is the gfp group
Wolfgang Schechinger
- Q: E.coli and Inositol Lipids?
Wolfgang Schechinger
- Q: Looking for GFP-cDNA
Wolfgang Schechinger
- FITC fading kinetics
Wolfgang Schechinger
- Qiagen Quiaquick : reliable? pure enough for Autoseq?
Wolfgang Schechinger
- re-using electroporation cuvettes
Claire Schimke
- The Biotech BiblioNet
Dyann K. Schmidel
- EMSA problems
Alexander Schmiechen
- SDS PAGE bluring
Denni Schnapp
- pipetting detergents
Denni Schnapp
- RNA Sequencing
Daniel R. Schoenberg
- PRINS / midisat human DNA
Manfred Schuermann
- Tricks for pipetting detergents??
Bart Sefton
- Invitrogen's pZERO & E.coli with ccdA
Bret Sellman
- Annealing of two complementary circular SS DNAs
Robert Seymour
- Other minipreps (was Re: Qiagen: This is ridiculous)
Robert Seymour
- Automated DNA Sequencing - better gel composition
Sudarshan Sharma
- ?urine RT-PCR
Dr. J.M. Sherwood
- PCR variability with DNA extraction method
benedicte Shevchenko
- PCR variability with DNA extraction method
benedicte Shevchenko
- Reasons for PCR failure....?
Joan Shields
- looking for a method to strip gel plates
Joan Shields
- anti fungal GAPDH antibodies?
Mari L. Shinohara
- Problems with ligation after GeneCleaning
Nori Shiraishi
- Label incorporation into DNA in vivo
Igor Sidorenkov
- Cloning tandem repeats
Richard Siegel
- trehalose determination
Sanford Silverman
- PromoterFinder Construction Kit
Gurmil Singh s/o Dalip Singh
- Removing endotoxin from plasmids
Mike Sklar
- chemical mutagenesis
Mark Slack
- Seamless Cloning
Dave Smith
- RNA PURIFICATION
Stephen Smith
- blunt end ligation problems
Wendy-Anne Smith
- Tricks for pipetting detergents??
Gary Smyly
- Cloning vector with kanamycin instead of ampicillin resistance
Kimberley Snowden
- Sections not sticking to slides
Kimberley Snowden
- Antibodies to poly-His
Keld Sorensen
- ELISA with 4-chloro-1-naphthol
Keld Sorensen
- gradient pcr?
Keld Sorensen
- SDS PAGE and phosphorylation
Keld Sorensen
- Westerns-blotting-BSA / E coli Ab
Keld Sorensen
- Detergent for Tissue Culture glassware
Keld Sorensen
- which imager?
Keld Sorensen
- smear Northern
J. David Spafford
- Harris -80 Freezers
J. David Spafford
- re-using electroporation cuvettes
Ralf Spenneberg
- sequence pTrcHis
Ralf Spenneberg
- NH4Ac-Miniprep becomes bad - why?
Ralf Spenneberg
- Which company sells Maxam-Gilbert reaction kit
Dom Spinella
- LiCl in DNA prep
Dom Spinella
- Rules of thumb for sequencing cloned PCR products
Dom Spinella
- PCR Detection of HLA Alleles
Dom Spinella
- RNA Sequencing
Otello Stampacchia
- WANTED: Yeast Library
John Stebbins
- Active Component in Skunk Urine
Stefan Einar Stefansson
- ConA - glycoproteins - ovalbumin
Steve
- Westerns-blotting-BSA
Steve
- Fiske and Subbarow reagent . . .
Dr. Timothy Strabala
- who makes 'OLIGO' software?
Steven Sullivan
- Reasons for PCR failure....?
Sailesh Surapureddi
- about transformation of DNA into E.Coli
Sailesh Surapureddi
- (none)
Sailesh Surapureddi
- DNA transformations into E.coli
Sailesh Surapureddi
- about competent cell
Sailesh Surapureddi
- 5 min transformation E. coli
Sailesh Surapureddi
- 5 min transformation E. coli
Sailesh Surapureddi
- Identification of a c-DNA clone
Sailesh Surapureddi
- who makes 'OLIGO' software?
Sailesh Surapureddi
- about transformation of DNA into E.Coli
Sailesh Surapureddi
- Double stranded dna Sequencing help
Vlad Svetlov
- Your basic stupid question about PCR cloning
Vlad Svetlov
- chemical mutagenesis
Vlad Svetlov
- No banding in CsCl Spin,Why?
Vlad Svetlov
- blunt end ligation problems
Vladimir Svetlov
- Microcentrifuge preferences???
Vladimir Svetlov
- Some polymerase better for PCR?
Vladimir Svetlov
- Cloning PCR products
Vladimir Svetlov
- PCR primer selection software
Vladimir Svetlov
- Qiagen: This is ridiculous
Vladimir Svetlov
- Reasons for PCR failure....?
Michal Svoboda
- MedImmune INc anyone ??
Ganesh Jawahar Swaminathan
- (no subject)
Antoinette Sweeney
- Chemifluorescence vs Chemiluminescence
TPM
- Genomic DNA prep
Paul Tadich
- dhfr activity assay?
Steven Tait
- GST fusions forming dimers - reference, PLEASE
Song Tan
- ANti human cytochrome b antibodies?
Shiping Tang
- Antibodies for human mt cytochrome b?
Shiping Tang
- Antibodies for human mt cytochrome b?
Shiping Tang
- pGex expression
Ron Tate
- ABI 310--help!
Agnes Tay
- AmpliTaq Gold vs AmpliTaq: where is the magic ?
Dr G R Taylor
- Kinetics software
Cristiana M.G. Teixeira
- Your basic stupid question about PCR cloning
Chromosome Terror
- Your basic stupid question about PCR cloning
Chromosome Terror
- I need a PROTOCOL !!!!
Tom Thatcher
- Epitope tags
Steven Theg
- Two hybrid library
Steven Theg
- U.S.E. mutagenesis
Didier Thomas
- GST-fusion expression problem
Didier Thomas
- FITC fading kinetics
Didier Thomas
- Shearing genomic DNA
Michael W. Thompson
- Cloning vector with kanamycin instead of ampicillin resistance
Michael W. Thompson
- Antibodies to 6-His Tag
Michael W. Thompson
- Tricks for pipetting detergents
Michael W. Thompson
- Hybond N Problems
Michael W. Thompson
- Detecting HISx6
Michael W. Thompson
- Eukaryotic protein expression
Michael W. Thompson
- Eukaryotic protein expression
Michael W. Thompson
- DNA electrophoresis
Michael W. Thompson
- Qiagen refuses to tell!
Michael W. Thompson
- Qiagen refuses to tell!
Michael W. Thompson
- Selection with puromycin
Michael W. Thompson
- Qiagen refuses to tell!
Michael W. Thompson
- Looking for different CAT assay protocols !
Phillip Thompson
- Antibodies to poly-His
Pat Thoyts
- Antibodies to 6-His Tag
Pat Thoyts
- SmaI digestion problems
Rosemary Thresher
- SmaI problems, correct address
Rosemary Thresher
- Test, please disregard
Tony
- BM non-radioactive probe labels?
Robert J. Toonen
- software for DNA and Protein comparision
Giang Tran
- pGex expression
Giang Tran
- Spectrophotometer
Krzysztof Treder
- Spectrophotometer
Krzysztof Treder
- (no subject)
Otha Tripp
- How to normalize
Gary Truett
- smear Northern
James Tsuruta
- Westerns-blotting-BSA
James Tsuruta
- I Need Advice on single cell differential display
Douglas B. Tully
- Sequence homology shareware
Douglas B. Tully
- Seamless Cloning->LIC (ligation independent cloning)
Bradley Turner
- Cloning tandem repeats
Bradley Turner
- Seamless Cloning->LIC (ligation independent cloning)
Bradley Turner
- Northern Normalization
Przemko Tylzanowski
- Oligo-services...
Josh Udall
- Sliver staining DNA
Josh Udall
- Q. gel photo documentation systems.
Josh Udall
- Size markers for sequencing gel
Paul Ulanch
- How many Kb in a centiMorgan????Help
Paul Ulanch
- PCR - SSCP loading mixture/denaturant??
Paul Ulanch
- Purification of VLP«s
Felix Unckell
- Carbenicillin
Dr. Derya Unutmaz
- I need a PROTOCOL !!!!
Virginia Urquidi
- about transformation of DNA into E.Coli
Edgar Valencia
- Hi Dr. SaileshIce transformation question
Edgar Valencia
- UV crosslinking of DNA to proteins
Luc Vallieres
- endotoxin assay kit
Marc Visconti
- Discrepancy between OD-value on spec and DNA on agarose gel
Botma Visser
- Thrombin Cleavage of GST
Dr A.H.M. Van Vliet
- sequence pTrcHis
Dr A.H.M. Van Vliet
- Biotin Pcr primers and anealing temperature
Karl Voss
- Biotin Pcr primers and anealing temperature
Karl Voss
- DNA Quantitation-Quick and Dirty
Karl Voss
- Biotin Pcr primers and anealing temperature
Karl Voss
- Antibodies directed against interferon
WENCENLAUS
- Linscott alternative?
WENCENLAUS
- Linscott alternative?
WENCENLAUS
- al antibodies against soybean BBI needed
WENCENLAUS
- contextual expression analysis
LMIB MULLERS WIL
- MICROEXTRACTION Embryo Typing
WSchick at aol.com
- Recomend Fluor. Plate reader?
WSchick at aol.com
- PCR Not Allowed
WSchick at aol.com
- gradient robocycler time savings
WSchick at aol.com
- DNA dye for fluorometric quantitation
WSchick at aol.com
- Qiagen refuses to tell! Proprietary information.
WSchick at aol.com
- Tricks for pipetting detergents??
Paul Wakenight
- Life on Mars
Andrew Wallace
- Recombinant ICE and CPP32
Andrew Wallace
- Recombinant ICE and CPP32/Apopain
Andrew Wallace
- Recombinant ICE and Apopain/CPP32
Andrew Wallace
- expressed protein is 5-6kda bigger than
Andrew Wallace
- Eukaryotic protein expression
Katie Walstrom
- Oligonucleotide purification
Edward Wang
- Mung bean nuclease ?
Edward Wang
- RAP PCR vs Differential display
Edward Wang
- RNA prep of Glomerular Core
Edward Wang
- Re- Cloning PCR Products
S. Wang
- expression vector
S. Wang
- Double stranded dna Sequencing help
Shiao Wang
- Some polymerase better for PCR?
Shiao Wang
- who makes 'OLIGO' software? - forget them! go with DNASTAR!
Pearse Ward
- DNA gel extraction
Pearse Ward
- Cellulose-substrate for Chromatography
Nadia Wechsler
- Help: genomic DNA purification
Chi-kuang Wen
- DNA gel extraction
Jon West
- pCMVCAT
Dieter Wiesmann
- In situ hybridization on tissue sections
D. P. Wijesinghe
- look for proof reading service
William
- YAC fragmentation vectors
Gareth Williams
- Reuse of Ni-columns?
Troels Wind
- lower detection limit with Coomssie?
Troels Wind
- Ni-chelation and Mg-binding
Troels Wind
- retroviral infection of lymphoblastoid cells
Kimberly Ann Winkeler
- Source for KlenTaq?
Johannes Winkler
- Experiences with HERMLE Microcentrifuges?
Johannes Winkler
- Large MW Standard f. SDSPAGE
Christoph H. Winter
- expressed protein is 5-6kda bigger than
Christoph H. Winter
- expressed protein is 5-6kda bigger than
Christoph H. Winter
- Accession # for Human and Rat beta-globin gene
Harry Witchel
- Cloning vector with kanamycin instead of ampicillin resistance
Harry Witchel
- Screening Libraries by PCR
Harry Witchel
- Disappearing plasmids
Arthur Wohlwill
- IsoQuick by Microprobe
H. Woo
- How to construct lab WWW page
Jim Woodgett
- Remove SDS from protein preparation
Chao-Liang Wu
- Q: PCR cloning from cDNA library
Jinling Xie
- ligation-mediated PCR
H YANG
- Reasons for PCR failure....?
Mayumi Yagi
- Rules of thumb for sequencing cloned PCR products?
Mayumi Yagi
- Vent polymerase from NEB
Chin-Fen Yang
- TEST
Feng Yang
- non-denaturing protein gel electrophoresis recipe?
Feng Yang
- blunt end ligation problems
HY Yang
- Detergent for Tissue Culture glassware
George Yeung
- Tricks for pipetting detergents??
H. Yim
- Tetracycline inducible system
Dong Yin
- EBV/LMP-1
Chui Yiu-Loon
- EBV/LMP-1
Chui Yiu-Loon
- No banding in CsCl Spin,Why?
Stephanie Yong
- Searching for oleosin probe
Stephanie Yong
- Smeary touchdown PCR gel.
Stephanie Yong
- "Translabel" isotopes
Ian A. York
- Somatic cell hybridization
Ian A. York
- Qiagen: This is ridiculous
Ian A. York
- Qiagen: This is ridiculous
Ian A. York
- Ultracentrifuge experiences?
Ian A. York
- DNA gel extraction
Taek H. You
- DNA gel extraction
Taek H. You
- RNA PURIFICATION
Taek H. You
- Northern Normalization
Taek H. You
- RT-PCR difficulty - PLEASE HELP!!!!!
Taek H. You
- Help with Genomic PCR
Taek H. You
- First Base, free molecular biology software exclusively for OS/2
Taek H. You
- LiCl in DNA prep
Taek H. You
- Sequencing directly from gel slices ?
Taek H. You
- RT-PCR difficulty - PLEASE HELP!!!!!
Taek H. You
- PKC assay in Hela cells
David Tak Yan Yu
- No banding in CsCl Spin,Why?
Zhiyong Zhao
- RNA Purification
Zhiyong Zhao
- problems with gel mobility shift assay
Zhang Zhiying
- Which company sell Maxam-Gilbert reaction kit?
Fanxiu Zhu
- DNA don't migrate...
Guan Zhu
- Thrombin Cleavage of GST
Guan Zhu
- whole mount in situ hybridization
a101anne at horus.sara.nl
- transcription termination
ae at dna.bio.warwick.ac.uk
- expressed protein is 5-6kda bigger than
ae at dna.bio.warwick.ac.uk
- Reasons for PCR failure....?
jason affourtit
- DNA dye for fluorometric quantitation
alex at alfred.med.monash.edu.au
- AmpliTaq Gold vs AmpliTaq: where is the magic ?
bb1dopeg
- AmpliTaq Gold vs AmpliTaq: where is the magic ?
bb1dopeg
- AmpliTaq Gold vs AmpliTaq: where is the magic ?
bb1dopeg
- yeast expression vector
bhalerao
- size of pBI 121?
dinakar bhattramakki
- DNA dye for fluorometric quantitation
bigcheez at ma.ultranet.com
- Lambda ZAP as a vector for bacterial libraries
billing at vetsci.microvet.arizona.edu
- expressed protein is 5-6kda bigger than
billing at vetsci.microvet.arizona.edu
- Cleaning Quartz Cuvettes
billing at vetsci.microvet.arizona.edu
- Carbos. in DNA prep!!!!!!
billing at vetsci.microvet.arizona.edu
- Temperature Sensitive pACYC or Derivatives
billing at vetsci.microvet.arizona.edu
- U937 cell extracts, retardation assay
bodammer at mailer.uni-marburg.de
- Affordable Patent Searching
breffni at neca.com
- SDS PAGE band blurring
brett
- No banding in CsCl Spin,Why?
brett
- Tricks for pipetting detergents??
brett
- Seamless Cloning->LIC (ligation independent cloning)
brett
- Eukaryotic protein expression
brett
- Qiagen: This is ridiculous
brett
- Concentrating proteins already in SDS sample buffer?
brett
- Bpu10I enzyme
carlo at florina.angers.inra.fr
- transgenic mice
carreer
- transgenic mice
carreer
- I wish radioactive colloidal chitin!!
choslab
- CFTR mutation analysis
Mike Morris sur cmusun12
- Spotty microsatellite sequencing gels:HELP (was: bind silane and sequencing)
craig.primmer at bmc.uu.se
- How many Kb in a centiMorgan????Help
dasuser at PO-Box.McGill.CA
- Using TdT to clean up sequencing reactions?
drdad at ktc.com
- Using TdT to clean up sequencing reactions?
drdad at ktc.com
- subscription
susan duthie
- Using TdT to clean up sequencing reactions?
edwardm at hhmi.ucla.edu
- Cleaning Quartz Cuvettes
edwardm at hhmi.ucla.edu
- lysis of RAW macrophage-like cell line
eheinecke at STUDENTS.WISC.EDU
- DNA gel extraction
ehwang at ucdavis.edu
- (none)
fotgar!fotgar.uba.ar!metodos at ccc.uba.ar
- Qiagen Quiaquick : reliable? pure enough for Autoseq?
futers at biovax.leeds.ac.uk
- Protocol for Drosphila genomic DNA prep
gc
- pBEND anyone?
gc
- Dyestuff-biotech stain
gem at pcnet.com
- Probes for rat ISH
kevin gilbride
- Postdoc position in New Zealand available
hraapg at marccri.marc.cri.nz
- PCR - SSCP loading mixture/denaturant??
Administrador de iihema.fmed.uba.ar
- GST-fusions and proteolysis
ijiwaru at wheel.dcn.davis.ca.us
- pGEX/GST gene fusion problems-> HELP!
ijiwaru at wheel.dcn.davis.ca.us
- Eukaryotic protein expression
ijiwaru at wheel.dcn.davis.ca.us
- Ligating small insert
ijiwaru at wheel.dcn.davis.ca.us
- RNA extraction
Farid jalali
- anti-heparin and EDTA PCR tricks
jbw.hammond at ukonline.co.uk
- does IPTG go bad???
jfh
- IEC micromax
jfh
- renaturing proteins
jgluz at STUD.MED.CORNELL.EDU
- Vector for direct cloning of PCR products
jlight at SICKKIDS.ON.CA
- Eukaryotic protein expression
jstrassw at OPAL.TUFTS.EDU
- test
jwobrien
- Glycogen for co-precipitating DNA
jwobrien
- smear Northern
kankaku
- HELP HELP HELP needed with RNA extraction
alex emil kentsis
- Shearing genomic DNA
Majumder lab
- Sections not sticking to slides
Majumder lab
- expressed protein is 5-6kda bigger than
lmcmurry_tet at opal.tufts.edu
- normalizing expression
lmrc.
- Nucleotide Sequence of pTich5
takashi matsumoto
- How determine target sequence of a novel DNA binding protein
mcatania at ucla.edu
- Beckman system gold nouveau
hassane mchaourab
- hemocyte marker?
mhlee
- How do I terminate transcription at a specific site in a plasmid that transfected into mammalian cells???
michael.baron at bbsrc.ac.uk
- Oligo-services...
milkman
- Transgenic reporter constructs
milstone
- Harris -80 Freezers
mpk6
- Sequence homology shareware
mpoulin at s-and-s.com
- who makes 'OLIGO' software? - forget them! go with DNASTAR!
mullenlynne at jsei.ucla.edu
- CAT
susanne müller
- sexing birds
nigel at mendel.berkeley.edu
- Harris -80 Freezers
nigel at mendel.berkeley.edu
- Searching desperately for pJM17 (E1 adenovirus)
noone
- DIG Northerns
nsaunders at molbiol.ox.ac.uk
- PCR - SSCP loading mixture/denaturant??
alex parker
- Direct Protection Assay Help...
john phelan
- DCOOD anhydrous: IS IT A DREAM?
piero pollesello
- BTX electroporator
johanna preiszner
- ISO: Next Plant Genome Meeting
johanna preiszner
- DNA Gel extraction
pudwell at qut.edu.au
- 2-hybrid library for Candida albicans?
john w. puziss
- size of pBI 121?
pzhang
- antibiotics for LBA4404
pzhang
- ref Quantitative PCR
raconte at ibm.net
- LiCl in DNA prep
rcowherd at mail.med.upenn.edu
- DNA don't migrate...
rcowherd at mail.med.upenn.edu
- Green Balancer ?
reichhart at astorg.u-strasbg.fr
- Green (GFP) Balancer (bis) ?
reichhart at astorg.u-strasbg.fr
- CAT gene
albano renee
- ssDNA->dsDNA transfos...was:annealing of ssDNA...
rhubner at molbiol.ox.ac.uk
- First Base, free molecular biology software exclusively for OS/2
rhughes at ucsd.edu
- First Base, free molecular biology software exclusively for OS/2
rhughes at ucsd.edu
- Staining gels in a microwave
rjanssen_imm at opal.tufts.edu
- about transformation of DNA into E.Coli
jane sandall
- chloramphenicol
p.j. van santbrink
- 3' prime radiolabeling of oligo using dideoxynucleotides
p.j. van santbrink
- Molecular biology software search
sczpjh at szn1.nott.ac.uk
- Qiagen: This is ridiculous
sharmaa at acsu.buffalo.edu
- Test. Pls. ignore
sharmaa at acsu.buffalo.edu
- Hybond N Problems
sunil shaw
- dsRNA Killer Virus M probe
sherif at DARWIN.UCSC.EDU
- T-vector
ssd2rc at po.iijnet.or.jp
- Quantitation of individual nucleotides
stein.bergan at labmed.uio.no
- looking for a method to strip gel plates
brad swanson
- Looking for different CAT assay protocols !
th.neumann at dkfz-heidelberg.de
- DNA electrophoresis
u.381 at inserm.u-strasbg.fr
- <None>
varyc.mmcwp4 at mmc.org
- gradient robocycler help
wegloehner at mpimg-berlin-dahlem.mpg.de
- PCR and food colors/dyes
wick at cris.com
- Please Help (pMAL)
wsa
- NIH-Image
Hongyu yang
- NIH-Image
Hongyu yang
- RAPD Trouble!!!
Hongyu yang
- Help: genomic DNA purification
Hongyu yang
- Need help with CAT assays in bacteria
yugtuyg
- who makes 'OLIGO' software? - forget them! go with DNASTAR!
zinc
- gradient robocycler help
zinc
- How to establish a stable pure line from transgenic plant?
§Ñ¼~¯ó
- Better method for deglycosylation...?
Àå°ÇÈñ
Last message date:
Sat Aug 31 15:12:12 EST 1996
Archived on: Sun Mar 6 21:10:22 EST 2005
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