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Methods August 1996 Archives by date

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Starting: Thu Aug 1 00:34:52 EST 1996
Ending: Sat Aug 31 15:12:12 EST 1996
Messages: 1169

Removing endotoxin from plasmids Mike Sklar
Dig-riboprobes and RNase A Gabriele Kerber
Dig-riboprobes and RNase A Gabriele Kerber
Antibodies to poly-His Keld Sorensen
ELISA with 4-chloro-1-naphthol Keld Sorensen
Help:ss-DNA Anja
gradient pcr? Keld Sorensen
Please Help (pMAL) wsa
SDS PAGE and phosphorylation Keld Sorensen
SDS PAGE bluring Denni Schnapp
carbenicillin Chang-Muk Lee
SmaI digestion problems Rosemary Thresher
SmaI problems, correct address Rosemary Thresher
Label incorporation into DNA in vivo Igor Sidorenkov
How many Kb in a centiMorgan????Help dasuser at PO-Box.McGill.CA
N-terminal His-tag expression vector Susana Galvez
test Minh Q. Nguyen
Qiagen DNA purification resin - what is is? Glenn Jenkins
Removing endotoxin from plasmids Mikhail Alexeyev
Looking for H. Bujard's Tet system plasmids: pBI-4 ... Fred Jacobs
SDS PAGE band blurring brett
Primer Design??? Fred Jacobs
isolation of DNA from mouse tails Seth Corey
Antibodies to poly-His IAN MCFARLANE
Antibody to CKR5 ? Mike Herron
Antibodies to poly-His Maria C. Costanzo
Anyone tried Sypro Gel Stains??? Mike Holbrook
fungal genomic DNA EGUARINOS at MVAX.CBM.UAM.ES
The Biotech BiblioNet Dyann K. Schmidel
PKC assay in Hela cells David Tak Yan Yu
Need Pyrimidine Dimer-DNA Glycosylase Scott Jelinsky
test Minh Q. Nguyen
Dyestuff-biotech stain gem at pcnet.com
Size markers for sequencing gel Paul Ulanch
How many Kb in a centiMorgan????Help Paul Ulanch
DNA Mass Ladder problems Bettina Lehnhardt
geneclean: vortexing Charles M Griswold
Removal of Ethidium Bromide Ed Seijo (BIO)
dhfr activity assay? Shanthi Adimoolam
Carbenicillin Torben Gjetting
trehalose determination Ed Bellion
DNA-quantification by software Andreas Becker
How to establish a stable pure line from transgenic plant? §Ñ¼~¯ó
Fluorescent Nucleotides Janice Kranz
4+2 rule Robert Darnell
Tetracycline inducible system Dong Yin
How many kb in a centiMorgan?? Graham Dellaire
catalase/tissue culture Tim Buss
X-gal staining without non-specific in the rat brain ? Sukwon Lee
Streck fixative for in situ Molecular Micro
Qiagen DNA purification resin - what is it? Cornelius Krasel
Oligonucleotide purification Edward Wang
SYBR Green II RNA coloration Isabelle Ernest
geneclean: vortexing Manuel G. CLAROS
Molecular biology software search Eitan Rubin
Reasons for PCR failure....? Bill Burnett
Primer Design??? Gary Kucera
"Translabel" isotopes Ian A. York
Reasons for PCR failure....? Martha L. Reed
"Translabel" isotopes David L. Haviland, Ph.D.
purity of urea for sequencing gels martin LEACH
Oligonucleotide purification martin LEACH
How many kb in a centiMorgan?? martin LEACH
Antibodies directed against interferon WENCENLAUS
Linscott alternative? WENCENLAUS
Linscott alternative? WENCENLAUS
Spots on Northern probed with oligos (50-mers) Andy Phillips
Reasons for PCR failure....? Sailesh Surapureddi
Double stranded dna Sequencing help Mozart de Azevedo Marins
UV crosslinking of DNA to proteins Simon Easterbrook-Smith
GST fusions forming dimers - reference, PLEASE Dima Klenchin
Comparing products Magazine Frederick Garbrecht
Freeze Shearing of DNA Steve Griffiths
Oligonucleotide purification Lynn Hughes
Oligonucleotide purification Lynn Hughes
Prodigisin Source? Stephen W. Mamber
Anyone tried Sypro Gel Stains??? Toumy Guettouche
test Allan Colthart
Reasons for PCR failure....? jason affourtit
human beta actin promoter in Euk exp. vector Richard Moldwin
Your basic stupid question about PCR cloning Frederick Garbrecht
Removal of Ethidium Bromide MELANSOND
Molecular biology software search Gary Kucera
BM non-radioactive probe labels? Robert J. Toonen
Your basic stupid question about PCR cloning martin LEACH
Reasons for PCR failure....? Susan Jane Hogarth
Problems with lipids Wolfgang Schechinger
How to "normalize" DREDD
Primer Design software Homepage DREDD
European Radiochemical suppliers Wolfgang Schechinger
Your basic stupid question about PCR cloning DREDD
How to "normalize" Buddha
Primer Design??? Buddha
Help with DD RT-PCR?? Alex Haddad
Q: Looking for GFP-cDNA Wolfgang Schechinger
Antibodies to poly-His Pat Thoyts
NIH Image advice... Dr Alexander J Annala
Contech Capfinder Michael_J_Hansbury
How many Kb in a centiMorgan????Help Daniel Mytelka
freeze-squeeze (Re: geneclean: vortexing) Trevor Bezdek
PRINS / midisat human DNA Manfred Schuermann
Anyone tried Sypro Gel Stains??? Michael Lassner
heme and binding Patrick Koppitz
How to normalize Gary Truett
Double stranded dna Sequencing help Immu-I
GST-fusions and proteolysis BIOTEC54
sexing birds GERALDINE KAMINSKI
promoter screening Leung Kwan Chi
sexing birds nigel at mendel.berkeley.edu
Harris -80 Freezers nigel at mendel.berkeley.edu
Chlorampehical inhibit ssDNA packaging? Dr. James E. Graham
Using ClaI for subcloning Dr. James E. Graham
Taq polymerase patent (not) Dr. James E. Graham
Staining gels in a microwave rjanssen_imm at opal.tufts.edu
normalizing expression lmrc.
Continuous gel ref Louis Hom
Taq polymerase patent (not) Tom Bickle
subcloning pcr bands L. Bastian
MCH in mammals Anabela Pinto Jensen
Primer Design??? Andreas Becker
Carbenicillin Dr. Derya Unutmaz
silver staining... Christian Radauer
BioRad CHEF DR-II Pulsed Field Gel Electrophoresis Apparatus Dr. Alexander J. Annala
about thermocycling sequencing Jin-seon Im
about squeez-freeze Jin-seon Im
Wanted: Mouse B-cell library Shane Albright
sexing birds Dr. James E. Graham
Evaluating reverse transcription Patrick HJ Falckh
Double stranded dna Sequencing help Vlad Svetlov
Chloropane ??? what the h... ??? Robot Boy
SDS PAGE band blurring Inyup Park
Oligonucleotide purification Daniel Jacobs
Does a cDNA clone contain a full length???????? Eitan Rubin
Does a cDNA clone contain a full length???????? Eitan Rubin
RAPD Trouble!!! Roland Koelliker
Your basic stupid question about PCR cloning Eitan Rubin
Q: E.coli and Inositol Lipids? Wolfgang Schechinger
Where is the gfp group Wolfgang Schechinger
test: please, ignore me ! Christine CARTIER
Antibody against c-jun Volker Noelle
GST fusions forming dimers - reference, PLEASE Chen Ho An
Accession # for Human and Rat beta-globin gene Mary P. Remington
PCR and food colors/dyes wick at cris.com
PCR variability with DNA extraction method P R Orange
RNA Quantification... Edouard Lauzier
Cellulose-substrate for Chromatography Nadia Wechsler
Total RNA labelling G.W.Phillips
<None> varyc.mmcwp4 at mmc.org
Reasons for PCR failure....? Mayumi Yagi
Harris -80 Freezers mpk6
Continuous gel ref Paul Digard
Cellulose-substrate for Chromatography Achim Recktenwald
SDS PAGE band blurring Achim Recktenwald
long PCR primer Chen Ho An
UV mutagenesis Steven Goldberg
Double stranded dna Sequencing help Jeremy Marcus
food colors in PCR Paul N. Hengen
trehalose determination Sanford Silverman
retroviral infection of lymphoblastoid cells Kimberly Ann Winkeler
Harris -80 Freezers Bruce Thomas Blakely
freeze-squeeze (Re: geneclean: vortexing) Jim Graham
NIH-Image Blanca Lupiani
sexing birds martin LEACH
lysis of RAW macrophage-like cell line eheinecke at STUDENTS.WISC.EDU
HELP HELP HELP needed with RNA extraction Paul A Bucciaglia
UV crosslinking of DNA to proteins Luc Vallieres
dsRNA Killer Virus M probe sherif at DARWIN.UCSC.EDU
Reasons for PCR failure Susan B. McLeskey
Need Mac NIH Grant Forms Alan Kaiser
Harris -80 Freezers Jeremy Marcus
Reasons for PCR failure....? Gerald Hamilton
HELP HELP HELP needed with RNA extraction alex emil kentsis
Cellulose-substrate for Chromatography Dima Klenchin
MICROEXTRACTION.
Q: E.coli and Inositol Lipids? C E Pical
pCMVCAT Dieter Wiesmann
Where is the gfp group Roberto Cappai
Chloropane ??? what the h... ??? Olivier Gandrillon
Help with PCR library screening John Brennand
MICROEXTRACTION. Fraser Dr N J
MICROEXTRACTION. Fraser Dr N J
Shearing genomic DNA Keith Grimaldi
MICROEXTRACTION. Matthew P. Frosch, M.D., Ph.D.
Q: E.coli and Inositol Lipids? Wolfgang Schechinger
normalizing expression K.Kramer
MICROEXTRACTION Embryo Typing WSchick at aol.com
Where is the gfp group Andy Phillips
Source for 3'-dCTP??? Alex Harvey
Need Mac NIH Grant Forms Scott Moye-Rowley
BTX electroporator johanna preiszner
pTrc99A-like plasmid... Pierre Olivier
Taq polymerase Alastair Hamilton
(none) Martin, Martin
Harris -80 Freezers Mark Farinha
lysis of RAW macrophage-like cell line Jim Graham
Recomend Fluor. Plate reader? Mike Herron
Enumerating adherent cells (w/o harvest)? Jim Graham
Reasons for PCR failure....? Jim Beasley
about Tag polymerase Jin-seon Im
Where is the gfp group Warren Casey
Where is the gfp group Warren Casey
Q: Looking for GFP-cDNA Wolfgang Schechinger
U937 cell extracts, retardation assay bodammer at mailer.uni-marburg.de
Oligo-services... David L. Haviland, Ph.D.
GST-fusions and proteolysis ijiwaru at wheel.dcn.davis.ca.us
Reasons for PCR failure....? Joan Shields
Your basic stupid question about PCR cloning Chromosome Terror
Your basic stupid question about PCR cloning Chromosome Terror
about transformation of DNA into E.Coli Jin-seon Im
about transformation of DNA into E.Coli Sailesh Surapureddi
Shearing genomic DNA Michael W. Thompson
about transformation of DNA into E.Coli Edgar Valencia
food colors in PCR Paul N Hengen
Nylon and Silver staining Pierre Commercon
Shearing genomic DNA Majumder lab
MutS gel mobility shift Les Hoffman
Source for KlenTaq? Johannes Winkler
Cloning tandem repeats Richard Siegel
reply: SSCP: Restriction Enzymes vs. Shorter Fragments Gregory S. Buzard 'Buz'
Q. gel photo documentation systems. Ken Kaufman
RNA Quantification... Allan Colthart
Test, please disregard Tony
Your basic stupid question about PCR cloning Vlad Svetlov
1997 FEBS Meeting on Cell Signalling Mechanisms Albert Noorlandt
smear Northern kankaku
E. coli host strain for transformation ?=4~$^
Uncouplers & pH Louis Hom
Help with RNA extraction method Karen Kroeger
about transformation of DNA into E.Coli Hidevaldo Bueno Machado
insect cell fusion Bill Langridge
Recomend Fluor. Plate reader? WSchick at aol.com
smear Northern Klaus Salger
IsoQuick by Microprobe H. Woo
Help with RNA extraction method Ned Mantei
sequencing MIcha Ron
RNA Quantification... Colin Dolphin
catalase/tissue culture Harold Drabkin
Large MW Standard f. SDSPAGE Christoph H. Winter
pCMVCAT Harutyan Melkonyan
Quantitation of individual nucleotides stein.bergan at labmed.uio.no
PCR variability with DNA extraction method benedicte Shevchenko
PCR variability with DNA extraction method benedicte Shevchenko
Accession # for Human and Rat beta-globin gene Harry Witchel
Cloning vector with kanamycin instead of ampicillin resistance Harry Witchel
Oligo-services... MELANSOND
about transformation of DNA into E.Coli MELANSOND
about squeez-freeze Vince Nguyen
(none) fotgar!fotgar.uba.ar!metodos at ccc.uba.ar
Oligo-services... milkman
Cloning vector with kanamycin instead of ampicillin resistance John Brennand
Genetrapper kit Dr N.J. Doyle
Copy number quantitation- PCR Daraporn Pittayakhachonwut
HELP:Ab against LuECAM Thomas Mohr
about transformation of DNA into E.Coli jane sandall
Green Balancer ? reichhart at astorg.u-strasbg.fr
Taq polymerase Robin Beech
(none) Sailesh Surapureddi
peptide syntheses Lisa Harrison-Bernard, PhD.
4+2 rule Eitan Rubin
(none) Fraser Dr N J
freeze-squeeze (Re: geneclean: vortexing) Eitan Rubin
Taq polymerase patent (not) Eitan Rubin
purity of urea for sequencing gels George Rutherford
Reasons for PCR failure....? George Rutherford
does IPTG go bad??? jfh
about transformation of DNA into E.Coli Jeremy Marcus
(none) Venkat Pisupati
X-gal staining without non-specific in the rat brain ? Riekeltje Koedood
Double stranded dna Sequencing help Shiao Wang
about transformation of DNA into E.Coli Paul N Hengen
Pointer to FAQ lists Paul N Hengen
Shearing genomic DNA Paul N Hengen
Epitope tags Steven Theg
Reasons for PCR failure....? Tom Deits
about competent cell Jin-seon Im
DNA transformations into E.coli Sailesh Surapureddi
Hi Dr. SaileshIce transformation question Edgar Valencia
Reasons for PCR failure....? :-Peter
Help with DD RT-PCR?? Louis Alarcon
about competent cell Sailesh Surapureddi
Two hybrid library Steven Theg
about transformation of DNA into E.Coli Paul N Hengen
RNA extraction Farid jalali
Reasons for PCR failure....? Gregory S. Buzard 'Buz'
inducible mammalian promoter Paul_J_Ryan
5 min transformation E. coli KHUANG at CHEMVX.TAMU.EDU
about transformation of DNA into E.Coli H. ROYCHOWDHURY
5 min transformation E. coli Sailesh Surapureddi
5 min transformation E. coli KHUANG at CHEMVX.TAMU.EDU
5 min transformation E. coli Sailesh Surapureddi
Identification of a c-DNA clone Rajesh Kumar
E. coli host strain for transformation .69g
Taq polymerase Jim Graham
look for proof reading service William
NIH-Image Hongyu yang
NIH-Image Hongyu yang
RAPD Trouble!!! Hongyu yang
Chemifluorescence vs Chemiluminescence TPM
Help: genomic DNA purification Hongyu yang
Help: genomic DNA purification Michelle Gleeson
Oligo-services... Josh Udall
DOW CHEMICAL JOINS GLOBAL CONSORTIUM ON ELECTRONIC LAB NOTEBOOKS CreateENBs at aol.com
DOW CHEMICAL JOINS GLOBAL CONSORTIUM ON ELECTRONIC LAB NOTEBOOKS CreateENBs at aol.com
T-vector ssd2rc at po.iijnet.or.jp
CFTR mutation analysis Mike Morris sur cmusun12
Reasons for PCR failure....? Eitan Rubin
Large MW Standard f. SDSPAGE Dr E. Buxbaum
Identification of a c-DNA clone Sailesh Surapureddi
PCR variability with DNA extraction method Dr Claudia Macaubas
Reasons for PCR failure....? Michal Svoboda
antibody against human L1 L. Griffith
GST fusions forming dimers - reference, PLEASE Song Tan
RNA extraction Fraser Dr N J
Oligo-services... Tonja Burshek
Life on Mars Andrew Wallace
Recombinant ICE and CPP32 Andrew Wallace
Sliver staining DNA Josh Udall
Oligo-services... Frank Murphy
Sliver staining DNA Paul N Hengen
Recombinant ICE and CPP32/Apopain Andrew Wallace
Screening Libraries by PCR Paul N Hengen
Mung bean nuclease ? Fourie Joubert
OD at 320nm Edouard Lauzier
CHO cells in suspension Ruth McMahon
bioluminescence-coelentrazine Luc Giasson
Uncouplers & pH L. Fliegel
HELP!!! Lambda prep... Allison
chemical mutagenesis Mark Slack
about transformation of DNA into E.Coli Paul N Hengen
who makes 'OLIGO' software? Steven Sullivan
about transformation of DNA into E.Coli Trevor Bezdek
RAP PCR vs Differential display Sandra Pena De Ortiz
Lambda ZAP as a vector for bacterial libraries billing at vetsci.microvet.arizona.edu
Disinfectant contact t & Mycobacterium tuberculosis Jim Graham
source for ICE or IchL Trevor Bezdek
Cloning vector with kanamycin instead of ampicillin resistance Klaus Salger
Q. gel photo documentation systems. Josh Udall
Green (GFP) Balancer (bis) ? reichhart at astorg.u-strasbg.fr
Screening Libraries by PCR Harry Witchel
who makes 'OLIGO' software? Sailesh Surapureddi
about transformation of DNA into E.Coli Sailesh Surapureddi
unsubscribe REGCHAK at USTHK.UST.HK
Automated DNA Sequencing - better gel composition Sudarshan Sharma
lobster genome size Michael Myers
(none) VASANTHARAJAN JANAKIRAMAN
about transformation of DNA into E.Coli H. ROYCHOWDHURY
size of pBI 121? pzhang
RT primer for quantitative PCR Kathleen Hopp
inducible mammalian promoter NETSCAPE APPLICATION
RNA-extraction from PAGE Detlef.Ritter at stud.uni-hannover.de
inducible mammalian promoter NETSCAPE APPLICATION
Help: genomic DNA purification Chi-kuang Wen
size of pBI 121? Mikhail Alexeyev
BioRad PROTEIN A BINDING BUFFER Alex Chang
Asparagine synthetase selection Masamichi Oshima
cDNA visualization on agarose, HELP!!! Gerardo Medrano
How to construct lab WWW page KHUANG at CHEMVX.TAMU.EDU
OD at 320nm Jose E. Mejia
Help with RNA extraction method George Rutherford
Cloning vector with kanamycin instead of ampicillin resistance Michael W. Thompson
about transformation of DNA into E.Coli Paul N Hengen
Cloning vector with kanamycin instead of ampicillin resistance Kimberley Snowden
WANTED: Yeast Library John Stebbins
Statistics and biology Edmundo Castro
10 %FCS Clemens Oliver Hanemann
A problem in topology Ed Beaty
chemical mutagenesis Vlad Svetlov
Discrepancy between OD-value on spec and DNA on agarose gel Botma Visser
Your basic stupid question about PCR cloning Dr. Duncan Clark
Sliver staining DNA D. KIM
Discrepancy between OD-value on spec and DNA on agarose gel MELANSOND
renaturing proteins jgluz at STUD.MED.CORNELL.EDU
A problem in topology Rafael Maldonado
Discrepancy between OD-value on spec and DNA on agarose gel Rafael Maldonado
To Sailesh Takbum Ohn
who makes 'OLIGO' software? - forget them! go with DNASTAR! zinc
RAPD Trouble!!! Eitan Rubin
Best prices of oligo synthesis - looking for details Eitan Rubin
dhfr activity assay? Steven Tait
vaccination with HIS tag protein Stephen J Billington
How to construct lab WWW page martin LEACH
DEHYDRATED CULTURE MEDIA $18/LB Robert Randolph
DEHYDRATED CULTURE MEDIA $18/LB David Kristofferson
Beckman system gold nouveau hassane mchaourab
How to construct lab WWW page Jim Woodgett
Grant Available - Genetic Engineering - Fungi Mark Emalfarb
Mung bean nuclease ? Edward Wang
RAP PCR vs Differential display Edward Wang
DNA gel extraction ehwang at ucdavis.edu
Genomic DNA prep Paul Tadich
Discrepancy between OD-value on spec and DNA on agarose gel Jim Hutchins
No banding in CsCl Spin,Why? Vlad Svetlov
Toxic fragment in pBR322 Stephan Geley
Help with nuclease S1 protocol Dinakar
No banding in CsCl Spin,Why? Stephanie Yong
contextual expression analysis LMIB MULLERS WIL
Searching for oleosin probe Stephanie Yong
Cholesterolfree FCS FABIAN MOEBIUS
PCR variability with DNA extraction method Frederick Garbrecht
HELP HELP HELP needed with RNA extraction Manuel G. CLAROS
No banding in CsCl Spin,Why? MELANSOND
How do I terminate transcription at a specific site in a plasmid that transfected into mammalian cells??? michael.baron at bbsrc.ac.uk
Help with nuclease S1 protocol (long!) Ned Mantei
long PCR kits Michael B. Coulthart
who makes 'OLIGO' software? Dave Knorr
No banding in CsCl Spin,Why? brett
OD at 320nm Achim Recktenwald
endotoxin assay kit Marc Visconti
blunt end ligation problems Jeremy C. Ahouse
Discrepancy between OD-value on spec and DNA on agarose gel H. ROYCHOWDHURY
renaturating protein Jin-seon Im
How do I terminate transcription at a specific site in a plasmid that transfected into mammalian cells??? Andrew Brooks
pGEX/GST gene fusion problems-> HELP! Brandon Harder
BEMITHYL: ITS MECHANISM AND ITS EFFECTS STEMC
pCMV-LacZ ? Sukwon Lee
endotoxin assay kit Leslie Carvalho
pGEX/GST gene fusion problems-> HELP! Mark Evans
(no subject) Otha Tripp
pGEX/GST gene fusion problems-> HELP! Michael DiDonato
VVND Leslie Carvalho
Somatic cell hybridization Ian A. York
Specificity of NEM toward cysteine residues Dominique DELFORGE
Invitrogen's pZERO & E.coli with ccdA Bret Sellman
RNA Sequencing Otello Stampacchia
Specificity of NEM toward cysteine residues Mikhail Alexeyev
MluI cuts like it wants Volker Noelle
Invitrogen's pZERO & E.coli with ccdA Mikhail Alexeyev
info on polyclonal antibodies against soybean BBI needed Vadim Beilinson
size of pBI 121? dinakar bhattramakki
Invitrogen's pZERO & E.coli with ccdA Mikhail Alexeyev
transcription termination ae at dna.bio.warwick.ac.uk
Statistics and biology John Ladasky
Kozak Sequence? Andy Peters
Indicators for electroporation eficiency Levan Darjania
Bind-Silane and sequencing Dr. Duncan Clark
Wedge Sequencing gels Dr. Duncan Clark
Tricks for pipetting detergents?? Paul Wakenight
What is: the toxic fragment in pBR322? Stephan Geley
Permenant mounting of AP-NBT/BCIP slides Wes Garrett
pGEX/GST gene fusion problems-> HELP! ijiwaru at wheel.dcn.davis.ca.us
Toxic fragment in pBR322 Bernard Murray
In situ hybridization on tissue sections D. P. Wijesinghe
Experiences with HERMLE Microcentrifuges? Johannes Winkler
Cosmids Stephen J Billington
pGEX/GST gene fusion problems-> HELP! Pete
Spectrophotometer Krzysztof Treder
Wedge Sequencing gels Andy Phillips
Gene knockout in E.coli Andy Morby
about transformation of DNA into E.Coli Alexander Kraev
Smeary touchdown PCR gel. Stephanie Yong
Bpu10I enzyme carlo at florina.angers.inra.fr
Gene knockout in E.coli P.R. Alefounder
Factor Xa Cleavage G E Martin
Taq DNA ligase and PCR P.R. Alefounder
subscription susan duthie
Sequence homology shareware mpoulin at s-and-s.com
Gene knockout in E.coli Mikhail Alexeyev
Re- Smeary Touchdown PCR Brett Beitzel
(no subject) Antoinette Sweeney
Reasons for PCR failure....? Susan Jane Hogarth
Tricks for pipetting detergents?? Glenn Jenkins
Factor Xa Cleavage Paul Digard
Cosmids J. Pat Martinez
Purification of VLP«s Felix Unckell
Formaldehyde aliquots Paula Marquardt
Mung bean nuclease ? Karah Lanier
Epitope tags: Methionines? Arle Kruckeberg
DIG-labelled RNA probes GILLIAN NI BHARLO BOTANY PG
Tricks for pipetting detergents?? brett
Position Open Technical Assistance
blood spot extractions Norm
Mung bean nuclease ? David Clarke
Microcentrifuge preferences??? Eric Roush
blood spots extractions Norm
Bind-Silane and sequencing H. ROYCHOWDHURY
No banding in CsCl Spin,Why? Zhiyong Zhao
Bind-Silane and sequencing Jeremy Marcus
DIG-labelled RNA probes Ed Beaty
Specificity of NEM toward cysteine residues Michael Galperin
Seamless Cloning Carolyn Hoyal-Wrightson
Bind-Silane and sequencing LouPass
Microcentrifuge preferences??? LouPass
"Translabel" isotopes LouPass
MluI cuts like it wants Richard J. Dudley
blunt end ligation problems Vladimir Svetlov
Microcentrifuge preferences??? Vladimir Svetlov
New at Experimental Medicine Job Listings!!! Graham Dellaire
Fiske and Subbarow reagent . . . Dr. Timothy Strabala
PCR Not Allowed WSchick at aol.com
HELP!!! Lambda prep... Atsushi Isoai
PCR Not Allowed David L. Haviland, Ph.D.
Has anyone separated proteins using agarose. John Hind
Tricks for pipetting detergents?? Wulf Dirk Leuschner
Fiske and Subbarow reagent . . . Dr E. Buxbaum
Molecular biology software search sczpjh at szn1.nott.ac.uk
DCOOD anhydrous: IS IT A DREAM? piero pollesello
expressed protein is 5-6kda bigger than Zheng Huanquan
Delta RNA Fingerprinting Kit Mary P. Remington
pipetting detergents Denni Schnapp
endotoxin assay kit Jan Hoolwerf
Thermostable DNA polymerase can be used in Taq DNA ligase buffer? Mr. Chris Hoyle
PCR Not Allowed Duke Groebe
Re- PCR not allowed Brett Beitzel
RPA, protect bigger band than probe Xin Guo
pGEX/GST gene fusion problems-> HELP! Mark Gosink
RPA, protect bigger band than probe Ned Mantei
Size fractionation of DNA John Brennand
Re- PCR not allowed H. ROYCHOWDHURY
Size fractionation of DNA Michael Feldman
Statistics and biology - a text book Eitan Rubin
No banding in CsCl Spin,Why? Jeremy Marcus
Microcentrifuge preferences??? Bernard Murray
Cosmids Peter J. Myler
Some polymerase better for PCR? Shiao Wang
IEC micromax jfh
expressed protein is 5-6kda bigger than ae at dna.bio.warwick.ac.uk
Re- PCR not allowed Bernard Murray
smear Northern J. David Spafford
Harris -80 Freezers J. David Spafford
Bind-Silane and sequencing Frank Murphy
Need help with CAT assays in bacteria Sarah K. Highlander, Ph.D.
Need help with CAT assays in bacteria Mikhail Alexeyev
DNA gel extraction Frederick Garbrecht
Liposomal transfection into human hematopoetic stem cells Frederick Garbrecht
Seamless Cloning Dave Smith
Bind-Silane and sequencing H. ROYCHOWDHURY
puromycin selection in e. coli and lymphoblastoid cell lines (LCLs) Ashok Aiyar
Some polymerase better for PCR? Vladimir Svetlov
Annealing of two complementary circular SS DNAs Lloyd Graham
help find peptide antisera service and information Vineet B Rajgarhia
Where to buy pentosidine? KC Dunn
PromoterFinder Construction Kit Gurmil Singh s/o Dalip Singh
DNA labelling Vivek Kumar
Statistics and biology - a text book Nigel Kenward
Problem with mtDNA-Degradation Frederik Intelmann
peptide/antibody production companies Guido Hooiveld
Microcentrifuge preferences??? Dr E. Buxbaum
Formaldehyde aliquots Dr E. Buxbaum
Has anyone separated proteins using agarose. Glenn Jenkins
Searching desperately for pJM17 (E1 adenovirus) Trevor Bezdek
PCR - SSCP loading mixture/denaturant?? alex parker
hybridization of expression lib James Warren Lafollette
Some polymerase better for PCR? Nick Jacobsen
Differential display kits - recommendation please Vernon Coyne
Vent polymerase from NEB Chin-Fen Yang
Bind-Silane and sequencing Jim Hutchins
Possible reasons for RAPD failure Roland Koelliker
RNA PURIFICATION MCCABE AJ
Annealing of two complementary circular SS DNAs Ken Howe
who makes 'OLIGO' software? - forget them! go with DNASTAR! mullenlynne at jsei.ucla.edu
who makes 'OLIGO' software? - forget them! go with DNASTAR! Pearse Ward
Postdoc position in New Zealand available hraapg at marccri.marc.cri.nz
DNA analysis program Mozart de Azevedo Marins
EIA detection of PCR products (streptoavidin capture) Jose Alejandro Palacios
Chloropane ??? what the h... ??? Josee Ash
Spectrophotometer Krzysztof Treder
PCR-SSCP loading mixture/denaturant?? Gregory S. Buzard 'Buz'
green fluorescent protein Michael Cooley
Antibodies to 6-His Tag Joseph Roland
Seamless Cloning->LIC (ligation independent cloning) Bradley Turner
Zoo blots Mr Hassan Abdulrazzak
I Need Advice on single cell differential display Douglas B. Tully
Sliver staining DNA Jacqueline Delp
Second hand zoo blots Mr Hassan Abdulrazzak
BioRad PROTEIN A BINDING BUFFER Jeffrey Bjorge
Cloning tandem repeats Bradley Turner
Taq data concealed?(was: Re-PCR not allowed) Bill Alexander
Seamless Cloning->LIC (ligation independent cloning) brett
Discrepancy between OD-value on spec and DNA on agarose gel Quentin E. Low
c600 David B. Knaebel
about transformation of DNA into E.Coli ProZyme, Inc.
Cloning vector with kanamycin instead of ampicillin resistance ProZyme, Inc.
Seamless Cloning->LIC (ligation independent cloning) Bradley Turner
Size fractionation of DNA ProZyme, Inc.
Demo DNA ProZyme, Inc.
(none) Thomas J. Jacks
S-35 DNA and differential display Gene Huh
Tricks for pipetting detergents Dan Coovert
Separating proteins on agarose Rosemary T. Hoffman
I need a PROTOCOL !!!! Victor Anaya-Baez
U.S.E. mutagenesis Jane.Wyatt.1 at massey.ac.nz
DNA gel extraction Taek H. You
(none) LIMARMG%BRUFMG.BITNET at UBVM.CC.BUFFALO.EDU
Annealing of two complementary circular SS DNAs Ned Mantei
DNA gel extraction W R BENNETT
RNA PURIFICATION Stephen Smith
al antibodies against soybean BBI needed WENCENLAUS
detection of PCR products by hydridization in streptoavidin coated plates Alejandro Palacios
A Test Manuel Palacios
AmpliTaq Gold vs AmpliTaq: where is the magic ? bb1dopeg
DNA gel extraction Pearse Ward
Has anyone separated proteins using agarose. Jim Hutchins
DNA gel extraction Taek H. You
RNA PURIFICATION Taek H. You
Hybond N Problems Mike Gruidl
Active Component in Skunk Urine Stefan Einar Stefansson
blunt end ligation problems HY Yang
HELP!!! Fungal contamination of IMPORTANT transfectants! Kiley R. Prilliman
blunt end ligation problems Morris Maduro
Tricks for pipetting detergents?? Robert Fjellstrom
Westerns-blotting-BSA / E coli Ab Keld Sorensen
who makes 'OLIGO' software? - forget them! go with DNASTAR! Rubin Eitan
GST-fusion expression problem Chris Houchens
HELP!!! Fungal contamination of IMPORTANT transfectants! Glenn Jenkins
Tricks for pipetting detergents?? Gary Smyly
Annealing of two complementary circular SS DNAs Robert Seymour
Antibodies to 6-His Tag Jongchan Lee
Antibodies to 6-His Tag Ashok Aiyar
Detecting HISx6 Mark Prescott
Antibodies to 6-His Tag AShaw5
expressed protein is 5-6kda bigger than AShaw5
Microcentrifuge preferences??? AShaw5
some polymerase better than Tag? Jin-seon Im
DNA gel extraction Jon West
Help me! / Yeast transformation in Two-Hybrid screening Takashi Nagano
Help - problem with bFGF PCR! Rebecca
who makes 'OLIGO' software? - forget them! go with DNASTAR! "Stephen A. Bustin 3123", SURG
Cloning PCR products Dr Andrew J. Doherty
Recombinant ICE and Apopain/CPP32 Andrew Wallace
Spotty microsatellite sequencing gels:HELP (was: bind silane and sequencing) craig.primmer at bmc.uu.se
Cloning PCR products Gary Kucera
RNA PURIFICATION Keith Leppard
chloramphenicol p.j. van santbrink
Reuse of Ni-columns? Troels Wind
expressed protein is 5-6kda bigger than Christoph H. Winter
Permenant mounting of AP-NBT/BCIP slides Paul C. Grimm MD
Re- Amplitaq Gold Brett Beitzel
RNA Sequencing Daniel R. Schoenberg
Flow Cytometry Protocols John MacDougall
Re- Cloning PCR Products Brett Beitzel
RNA Purification MCCABE AJ
yeast expression vector bhalerao
RNA Purification Zhiyong Zhao
RNA Purification Michael Kertesz
AmpliTaq Gold vs AmpliTaq: where is the magic ? Bernard Murray
HELP!!! Fungal contamination of IMPORTANT transfectants! Richard J. Dudley
Antibodies to 6-His Tag Michael W. Thompson
Tricks for pipetting detergents Michael W. Thompson
Hybond N Problems Michael W. Thompson
Detecting HISx6 Michael W. Thompson
hnRNP isolation Michael Robert Ladomery
Detecting HISx6 TJ Murphy
Tricks for pipetting detergents?? H. Yim
Bind-Silane and sequencing Rafael Maldonado
Problems with ligation after GeneCleaning Steven Goldberg
Inserting cDNA into promoter constructs??? Obaid Y. Khan
Custom BAC libraries? STACY A CIUFO
chloramphenicol Bernard Murray
Cosmid isolation Dubear Kroening
TEST Feng Yang
Hybond N Problems sunil shaw
non-denaturing protein gel electrophoresis recipe? Feng Yang
Re- Cloning PCR Products S. Wang
Q: PCR cloning from cDNA library Jinling Xie
expression vector S. Wang
DNA gel extraction Michelle Gleeson
Hybond N Problems Paul N Hengen
DNA dye for fluorometric quantitation alex at alfred.med.monash.edu.au
Plasmid pKK3535? Michael Casey
Tricks for pipetting detergents?? Bart Sefton
AMBIONS ANTISENSE 18S TEMPLATE Jennifer L Causey
AMBIONS ANTISENSE 18S TEMPLATE Jennifer L Causey
AMBIONS ANTISENSE 18S TEMPLATE Jennifer L Causey
detection of PCR products by hydridization in streptoavidin coated plates Rick Nicholson
Cloning PCR products Vladimir Svetlov
Demo DNA Rosnthorn at aol.com
EBV/LMP-1 Chui Yiu-Loon
EBV/LMP-1 Chui Yiu-Loon
removing fungal contamination from cell cultures Michelle Gleeson
Gram-positive sequence similarities-info req Maria Bobrowska
Discrepancy between OD-value on spec and DNA on agarose gel Louis Geller
anti-heparin and EDTA PCR tricks Dan Andrews
Antibody contamination during immunoaffinity purification Dooyeon Kim
Cy5 Custom DNA Synthesis Dr Alan Chan
who makes 'OLIGO' software? - forget them! go with DNASTAR! Andy Law
Re- Cloning PCR Products Andrew J. Doherty
Cy5 Custom DNA Synthesis Dr Michael Morris
E.coli cultures lysing after induction Peter Ashton
Annealing of two complementary circular SS DNAs Joe Boutell
chloramphenicol John Dixon
Problems with ligation after GeneCleaning MELANSOND
RT-PCR M.MECRIN
Stains-all: Protein staining Scott Ripley.
Annealing of two complementary circular SS DNAs Ned Mantei
Cloning PCR products Ashok Aiyar
CAT gene albano renee
Cloning PCR products Volker Blaschke
test jwobrien
Glycogen for co-precipitating DNA jwobrien
Optimum conditions for Mitochondrial PCR Tara Paton
anti-heparin and EDTA PCR tricks jbw.hammond at ukonline.co.uk
E.coli cultures lysing after induction Brent Pollock
Test don't read Rodney Earl Pettway
Problems with ligation after GeneCleaning Yoonsuk Park
AmpliTaq Gold vs AmpliTaq: where is the magic ? Dr G R Taylor
AMBIONS ANTISENSE 18S TEMPLATE Bernard Murray
ConA - glycoproteins - ovalbumin Steve
Which company sell Maxam-Gilbert reaction kit? Fanxiu Zhu
RT-PCR Gary Kucera
problems with gel mobility shift assay Zhang Zhiying
Eukaryotic protein expression ijiwaru at wheel.dcn.davis.ca.us
Ligating small insert ijiwaru at wheel.dcn.davis.ca.us
Glycogen for co-precipitating DNA Paul N Hengen
expressed protein is 5-6kda bigger than Paul N Hengen
Director Level Position Martin Blankfard
DNA gel extraction Paul N Hengen
2-hybrid library for Candida albicans? john w. puziss
Antibodies to 6-His Tag Pat Thoyts
Direct Protection Assay Help... john phelan
Hybond N Problems Mike Gruidl
Using TdT to clean up sequencing reactions? edwardm at hhmi.ucla.edu
Ligating small insert Minh Q. Nguyen
Antibody contamination during immunoaffinity purification Bernard Murray
glycerol to avoid protein precipitation?... Arioch
Using TdT to clean up sequencing reactions? drdad at ktc.com
DNA Gel extraction pudwell at qut.edu.au
Eukaryotic protein expression Katie Walstrom
I need a PROTOCOL !!!! Rover
smear Northern James Tsuruta
Cy5 Custom DNA Synthesis Dr Alan Chan
Cy5 Custom DNA Synthesis Dr Alan Chan
Northern Normalization Susan Patrick
How determine target sequence of a novel DNA binding protein mcatania at ucla.edu
Second hand zoo blots Imgenfm
U.S.E. mutagenesis Didier Thomas
glycerol to avoid protein precipitation?... Cornelius Krasel
Northern Normalization Przemko Tylzanowski
GST-fusion expression problem Didier Thomas
Probes for rat ISH Amanda Marsh
Glycogen for co-precipitating DNA Frederik Intelmann
Test, Please Ignore Gary Kucera
removing fungal contamination from cell cultures Robert Randolph
Help me! / Yeast transformation in Two-Hybrid screening Joe Boutell
I Need Advice on single cell differential display Paul C. Grimm MD
Ligating small insert Gary Kucera
expressed protein is 5-6kda bigger than Christoph H. Winter
I need a PROTOCOL !!!! Virginia Urquidi
glycerol to avoid protein precipitation?... Achim Recktenwald
test Rodney Earl Pettway
Carbos. in DNA prep!!!!!! Rodney Earl Pettway
Northern Normalization Heinz-Juergen Schaefers
Sections not sticking to slides GILLIAN NI BHARLO BOTANY PG
Using TdT to clean up sequencing reactions? David Micklem
PCR INHIBITOR M.MECRIN
DNA Quantitation-Quick and Dirty David Barrass
Yeast cDNA bank in yeast vector? Aletta Karsies
E.coli cultures lysing after induction Hong Hung
Eukaryotic protein expression brett
Tricks for pipetting detergents?? Stephen P. Driska PhD
Eukaryotic protein expression jstrassw at OPAL.TUFTS.EDU
RT-PCR difficulty - PLEASE HELP!!!!! Tonja Burshek
Spotty microsatellite sequencing gels:HELP (was: bind silane and sequencing) Paul Hollenbach
expressed protein is 5-6kda bigger than Paul N Hengen
ISO: Next Plant Genome Meeting johanna preiszner
expressed protein is 5-6kda bigger than billing at vetsci.microvet.arizona.edu
Which company sell Maxam-Gilbert reaction kit? Riekeltje Koedood
Cheap Eporation Cuvettes? Bob Belas
Which company sells Maxam-Gilbert reaction kit Dom Spinella
I need a PROTOCOL !!!! Tom Thatcher
ANti human cytochrome b antibodies? Shiping Tang
Antibodies for human mt cytochrome b? Shiping Tang
Antibodies for human mt cytochrome b? Shiping Tang
Mutagenesis: Does QuickChange (TM) work well? Stefan Kahlert
What really indicates induction? Susan Patrick
Microcentrifuge preferences??? DSF
Tricks for pipetting detergents?? DSF
RNA prep of Glomerular Core Edward Wang
DNA extraction from BAC clone Hiroshi Inoue
Sections not sticking to slides Kimberley Snowden
Help with Genomic PCR Miguel Cabrita
blunt end ligation problems Wendy-Anne Smith
DNA extraction from BAC clone Michael J. Prigge
Detergent for Tissue Culture glassware George Yeung
Active Component in Skunk Urine Dianne Emslie
anti fungal GAPDH antibodies? Mari L. Shinohara
Help me! / Yeast transformation in Two-Hybrid screening Michael Hamilton
Northern Normalization Ned Mantei
What really indicates induction? Rick Nicholson
Problems with ligation after GeneCleaning Gabby Gerlitz
Problems with ligation after GeneCleaning Nico Dantuma
(none) Masato Kinoshita
Fugu and zebrafish DNA D.D. Morgan
Cy5 Custom DNA Synthesis K.K.Chan
Test K.K.Chan
Test K.K.Chan
Cy5 Primers K.K.Chan
Cy5 Primers K.K.Chan
Make Your Own Vacuum Pump for Gel Blotting! Robert Passey
expressed protein is 5-6kda bigger than Zheng Huanquan
Tet-off/Tet-on system Philippe Lefebvre
plasmid curing M.De-Ungria at unsw.edu.au
Problems with ligation after GeneCleaning Anis Limami
Problems with ligation after GeneCleaning Anis Limami
INFORMATION NEEDED -Plant Antioxidants- Anis Limami
AMBIONS ANTISENSE 18S TEMPLATE K.Kramer
RT-PCR difficulty - PLEASE HELP!!!!! John Dixon
what conc antibiotics for Carb/Kan/Tet cocktail? John Dixon
Tricks for pipetting detergents?? Dr E. Buxbaum
glycerol to avoid protein precipitation?... Dr E. Buxbaum
I need a promoter selection vector, help Merja.Mikkonen at csc.fi
Problems with ligation after GeneCleaning James Warren Lafollette
Biotin Pcr primers and anealing temperature Karl Voss
Active Component in Skunk Urine Gerald Forrest
Hybond N Problems George Rutherford
blunt end ligation Wulf Dirk Leuschner
Bacterially Expressed Proteins: IPTG induction Jay L. Rothstein
What really indicates induction? Riekeltje Koedood
Biotin Pcr primers and anealing temperature John Dixon
Westerns-blotting-BSA Steve
Vector for direct cloning of PCR products jlight at SICKKIDS.ON.CA
Eukaryotic protein expression Michael W. Thompson
Eukaryotic protein expression Michael W. Thompson
Sections not sticking to slides Wulf Dirk Leuschner
expressed protein is 5-6kda bigger than Wulf Dirk Leuschner
Molecular biology software search Wulf Dirk Leuschner
Westerns-blotting-BSA Bernard Murray
antibody to CKR5? Mike Herron
Sections not sticking to slides Majumder lab
Plasmid amplification in Cm-r E.coli Masayoshi Itoh
U.S.E. mutagenesis Tim Kirksey
Kinetics software Cristiana M.G. Teixeira
Problems with ligation after GeneCleaning Jeremy Marcus
Cre exprssing E. coli strain Yasushi Okazaki
Cleaning Quartz Cuvettes billing at vetsci.microvet.arizona.edu
glycerol to avoid protein precipitation?... Toumy Guettouche
M1/70 Ab and Western Blots 'Fumi' Fumitaka Hayashi
Active Component in Skunk Urine Edward Theodore Michelini
LambdaGEM 11 Xho gLibrary Michelle Gleeson
Biotin Pcr primers and anealing temperature Dan Andrews
PCR primer selection software Vladimir Svetlov
FITC fading kinetics Didier Thomas
PCR primer selection software LAU Tze Chin
U.S.E. mutagenesis Mark Brandt, Ph.D.
Sequence homology shareware Douglas B. Tully
First Base, free molecular biology software exclusively for OS/2 rhughes at ucsd.edu
B.napus genomic library Peta C. Bonham-Smith
Poly A Northern normalisation Peta C. Bonham-Smith
software for DNA and Protein comparision Giang Tran
Smeary touchdown PCR gel. Dietmar
Formaldehyde aliquots MELANSOND
Cleaning Quartz Cuvettes MELANSOND
Need help with CAT assays in bacteria yugtuyg
Active Component in Skunk Urine Dan Johnson
expressed protein is 5-6kda bigger than Andrew Wallace
Help-Lg-scale Bacterial Genomic DNA Prep Joel Bader
Carbos. in DNA prep!!!!!! Joel Bader
Biotin Pcr primers and anealing temperature Duke Groebe
Searching desperately for pJM17 (E1 adenovirus) noone
Eliminate light/heavy chain after IP?? Danny Altschuler
Cleaning Quartz Cuvettes Dr E. Buxbaum
Qiagen Quiaquick : reliable? pure enough for Autoseq? Matt Hudson
re-using electroporation cuvettes Claire Schimke
FITC fading kinetics Graham Atherton
re-using electroporation cuvettes Ralf Spenneberg
Qiagen Quiaquick : reliable? pure enough for Autoseq? futers at biovax.leeds.ac.uk
Westerns-blotting-BSA Robert Hindges
LiCl in DNA prep Rodney Earl Pettway
expressed protein is 5-6kda bigger than lmcmurry_tet at opal.tufts.edu
EMSA problems Alexander Schmiechen
Eliminate light/heavy chain after IP?? Andrei Popov
MedImmune INc anyone ?? Ganesh Jawahar Swaminathan
truncated vector Alexander N. Kukushkin
Biotin Pcr primers and anealing temperature Karl Voss
re-using electroporation cuvettes Justinian
Church buffers (Was Re: Hybond N) Mark Jordan
re-using electroporation cuvettes Ashok Aiyar
Molecular biology software search Bernard Murray
Church buffers (Was Re: Hybond N) Paul N Hengen
Ligating small insert S.L. Rennie
AmpliTaq Gold vs AmpliTaq: where is the magic ? bb1dopeg
LiCl in DNA prep Dom Spinella
Biotin Pcr primers and anealing temperature Timothy R Jackson
software for DNA and Protein comparision Jack Goff
pGEX/GST gene fusion problems-> HELP! Paul N Hengen
chloramphenicol P.Bundock
Cleaning Quartz Cuvettes edwardm at hhmi.ucla.edu
Sequencing directly from gel slices ? Bryan L. Ford
Westerns-blotting-BSA James Tsuruta
glycerol to avoid protein precipitation?... Bill Prichett
CAT gene -- fluorescent substrates? Gene Huh
EMBO/ICGEB Phage Display Course Paul N Hengen
Northern Normalization Taek H. You
RT-PCR difficulty - PLEASE HELP!!!!! Taek H. You
Help with Genomic PCR Taek H. You
Bacterially Expressed Proteins: IPTG induction Zheng Huanquan
First Base, free molecular biology software exclusively for OS/2 Taek H. You
LiCl in DNA prep Taek H. You
Sequencing directly from gel slices ? Taek H. You
BIOSCI/bionet miniFAQ & Fundraiser BIOSCI Administrator
DNA contanimate Gabby Gerlitz
ISO: Next Plant Genome Meeting Roy G. Cantrell
Probes for rat ISH kevin gilbride
FITC fading kinetics Wolfgang Schechinger
Qiagen Quiaquick : reliable? pure enough for Autoseq? Wolfgang Schechinger
Help me! / Yeast transformation in Two-Hybrid screening Sourav Ghosh
Qiagen: This is ridiculous Ian A. York
Tricks for pipetting detergents?? Piotr Kozbial
Northern Normalization Bernard Murray
Qiagen: This is ridiculous Vladimir Svetlov
Codons Usage software Francisco Uribe Romeo
Problems with ligation after GeneCleaning Nori Shiraishi
LambdaGEM 11 Xho gLibrary Carsten Hoege
Problems with ligation after GeneCleaning Chris Houchens
Qiagen: This is ridiculous brett
DNA contanimate MELANSOND
Qiagen: This is ridiculous Nikolai Chitaev
AmpliTaq Gold vs AmpliTaq: where is the magic ? Steve Griffiths
RNA sizing against DNA markers GILLIAN NI BHARLO BOTANY PG
RNA sizing against DNA markers Bernard Murray
Transgenic reporter constructs Sandra Pena De Ortiz
Qiagen: This is ridiculous Ian A. York
Detergent for Tissue Culture glassware Dima Klenchin
bacterially expressed protein Jin-seon Im
pGex expression Giang Tran
Southern Blotting of Pulse Field Gels.... HELP!!? Graham Dellaire
RNA Centrifuge Spins Patrick HJ Falckh
Transgenic reporter constructs Bernard Murray
poly-A purification Szabadkai Gyorgy
DNA don't migrate... Jukka U Palo
DNA contanimate M.De-Ungria at unsw.edu.au
Southern Blotting of Pulse Field Gels.... HELP!!? Pascal Cousin
YAC fragmentation vectors Gareth Williams
How to denature target DNA on nylon membrane? Jeremy Chen
UV safe TAE ? Rico Laage
unsubscribe Woo-Jai Lee
glycerol to avoid protein precipitation?... Achim Recktenwald
myoglobin cDNA Louis Alarcon
Yeast contamination on cell culture:HELP!! Xose S. Puente
Loss of polymorphism Pooba Ganeshan
Kinetics software Achim Recktenwald
DNA contanimate Achim Recktenwald
LiCl in DNA prep H. ROYCHOWDHURY
gradient robocycler help wegloehner at mpimg-berlin-dahlem.mpg.de
ligation-mediated PCR H YANG
Qiagen: This is ridiculous Tsichlis Lab
DNA size markers Dr. Douglas A. Johnson
MedImmune INc anyone ?? martin LEACH
gradient robocycler help martin LEACH
Detergent for Tissue Culture glassware IAN MCFARLANE
GENOMIC SOUTHERN: help with DNAseI Hypersensitivity (please!!!!!) (: Heidi Moss
test Lee, Sun
PCR - SSCP loading mixture/denaturant?? Paul Ulanch
Transgenic reporter constructs milstone
cDNA sequencing Islam Khan
cleavage of MBP fusion proteins Guriq Basi
pGEX/GST gene fusion problems-> HELP! Jeanne McAdara
pGex expression Ron Tate
antibiotics for LBA4404 pzhang
Qiagen: This is ridiculous Jeanne McAdara
LiCl in DNA prep rcowherd at mail.med.upenn.edu
DNA Quantitation-Quick and Dirty Karl Voss
Protocol for Drosphila genomic DNA prep gc
DNA Quantitation-Quick and Dirty Yoonsuk Park
Cycle sequence results? Eric W. Linton
gradient robocycler help zinc
gradient robocycler help Jill Kellogg
Bacterially Expressed Proteins: IPTG induction Ted Michelini
antibiotics for LBA4404 Byeong-ha Lee
Qiagen: This is ridiculous Susan Jane Hogarth
gradient robocycler help Kiley R. Prilliman
cleavage of MBP fusion proteins Mark Brandt, Ph.D.
PCR - SSCP loading mixture/denaturant?? Administrador de iihema.fmed.uba.ar
pGEX/GST gene fusion problems-> HELP! Steve Cohen
Qiagen: This is ridiculous sharmaa at acsu.buffalo.edu
Test. Pls. ignore sharmaa at acsu.buffalo.edu
HELP nuclear runon nuclear runoff Anis Limami
Thrombin Cleavage of GST Evan Burkala
?urine RT-PCR Dr. J.M. Sherwood
Nucleotide Sequence of pTich5 takashi matsumoto
DNA contanimate Joe Boutell
Roche AMPLICOR HCV PCR GERALDINE KAMINSKI
DNA contanimate Halil-Cem Guersoy
biotin labelling Joachim Kremerskothen
Qiagen buffer contents Fredrik Kamme
source of annexin Joanna Aretz
Qiagen refuses to tell! Fredrik Kamme
Rules of thumb for sequencing cloned PCR products? Mark Pallen
AmpliTaq Gold vs AmpliTaq: where is the magic ? bb1dopeg
Rules of thumb for sequencing cloned PCR products Dom Spinella
CAT gene -- fluorescent substrates? Ed Beaty
UV safe TAE ? Ed Beaty
Plasmid amplification in Cm-r E.coli DAVE BOYD
Bacterially Expressed Proteins: IPTG induction Randy Nonay
Tricks for pipetting detergents Sarah King
Southern Blotting of Pulse Field Gels.... HELP!!? Nick Jacobsen
Using TdT to clean up sequencing reactions? drdad at ktc.com
Rules of thumb for sequencing cloned PCR products? Mayumi Yagi
Ultracentrifuge experiences? Ian A. York
Yeast contamination on cell culture:HELP!! Richard J. Dudley
Biotin Pcr primers and anealing temperature Karl Voss
pGEX/GST gene fusion problems-> HELP! Michael DiDonato
DNA don't migrate... Guan Zhu
RNA sizing against DNA markers D. KIM
Better method for deglycosylation...? Àå°ÇÈñ
Yeast contamination on cell culture:HELP!! Heinz-Juergen Schaefers
standard for northern blot analysis Dr. Benoit Gauthier
MOLCULAR WEIGHT MARKERS Doug Johnson
Ribozyme construction John MacDougall
(none) Martin Daoust
Sequencing PCR product by degenerateprimer KHUANG at CHEMVX.TAMU.EDU
SV40 vs CMV promoter Marc Rosoff
looking for a method to strip gel plates brad swanson
Thrombin Cleavage of GST AShaw5
pGex expression AShaw5
Thrombin Cleavage of GST Lyle Najita
Glycogen for co-precipitating DNA Edmundo Castro
DNA don't migrate... rcowherd at mail.med.upenn.edu
UV safe TAE ? Rafael Maldonado
looking for a method to strip gel plates Michelle Gleeson
CAT gene Tim Barnett
HELP nuclear runon nuclear runoff Rick Nicholson
gradient robocycler help MichaelCataniamcatania at ucla.edu
I wish radioactive colloidal chitin!! choslab
RT-PCR difficulty - PLEASE HELP!!!!! Taek H. You
looking for a method to strip gel plates Gene Huh
First Base, free molecular biology software exclusively for OS/2 rhughes at ucsd.edu
Broad-range host for E.coli phages Peter Kuhnert
Test - no message Mike Challen
UV safe TAE ? Rico Laage
Yeast contamination on cell culture:HELP!! Joao Vasconcelos Costa
Detergent for Tissue Culture glassware Keld Sorensen
Qiagen: This is ridiculous Nigel Kenward
Yeast contamination on cell culture:HELP!! Stephane Ory
hydropathy Oliver S. DePeyer
antibiotics for LBA4404 Matt Hudson
Westerns-blotting-BSA Matt Hudson
Annealing of two complementary circular SS DNAs Chris Boyd
3' prime radiolabeling of oligo using dideoxynucleotides p.j. van santbrink
Looking for different CAT assay protocols ! th.neumann at dkfz-heidelberg.de
looking for a method to strip gel plates MELANSOND
PCR detection of HLA alleles Robin Beech
RT-PCR difficulty - PLEASE HELP!!!!! Tonja Burshek
DNA contanimate Birgit Jostes
CAT susanne müller
Thrombin Cleavage of GST Guan Zhu
How to denature target DNA on nylon membrane? CRH
Looking for antibodies against Cre-recombinase Thomas R. Anderson
Thrombin Cleavage of GST Dr A.H.M. Van Vliet
sequence pTrcHis Dr A.H.M. Van Vliet
looking for a method to strip gel plates H. ROYCHOWDHURY
DNA-binding protein data file? Jean-Francois Larrivee
transgenic mice carreer
DNA-binding protein question Jean-Francois Larrivee
Church buffers (Was Re: Hybond N) Alexander Kraev
Sequencing directly from gel slices ? Alexander Kraev
PCR Detection of HLA Alleles Dom Spinella
Transgenic reporter constructs Riekeltje Koedood
Other minipreps (was Re: Qiagen: This is ridiculous) Victor Levenson
Qiagen: This is ridiculous Frank Murphy
Need Help with Cos-7 Transfection and Transit Matthew Kennedy
UV Safe TAE Les Hoffman
Northern Normalization Ken Dwyer
How to construct lab WWW page MMCCARTHY at BIOTECHNET.COM
looking for a method to strip gel plates Joan Shields
BIOMETRA - Thermoblock ? Michael Casey
looking for a method to strip gel plates Karl Fischer
Looking for different CAT assay protocols ! Phillip Thompson
Carbos. in DNA prep!!!!!! billing at vetsci.microvet.arizona.edu
Qiagen: This is ridiculous/purity Richard A. Janssen
Looking for different CAT assay protocols ! Richard A. Janssen
Does Taq Stoffel fragment produce 3' A overhangs. Michelle Gleeson
gradient robocycler time savings WSchick at aol.com
PCR detection of HLA alleles John Ladasky
Other minipreps (was Re: Qiagen: This is ridiculous) Robert Seymour
sequence pTrcHis Ralf Spenneberg
Looking for anti-CD9 and CD63 abs Yves Poumay
UV Safe TAE Ned Mantei
Does Taq Stoffel fragment produce 3' A overhangs. Jamie
DNA size markers Dr. Duncan Clark
Sequencing PCR product by degenerateprimer *
Pipette for PCR Chui Yiu Loon
NH4Ac-Miniprep becomes bad - why? *
Patent on GeneClean? *
Patent on GeneClean? *
Looking for different CAT assay protocols ! Keith Leppard
XL-1 Red Mutagenesis FABIAN MOEBIUS
transgenic mice carreer
which imager? Keld Sorensen
Qiagen: This is ridiculous Oliver S. DePeyer
Insect cell electroporation Mike Holbrook
NH4Ac-Miniprep becomes bad - why? H. ROYCHOWDHURY
(none) Martin Daoust
Temperature Sensitive pACYC or Derivatives billing at vetsci.microvet.arizona.edu
which phophoImager? Riekeltje Koedood
Elongase vs. Expand: Which is better? Miguel Cabrita
NH4Ac-Miniprep becomes bad - why? Jose E. Mejia
MOLCULAR WEIGHT MARKERS Pamela Norton
Trypsinization and NRO Vince Nguyen
Nuclear run-off with DIG-UTP Vince Nguyen
Carbos. in DNA prep!!!!!! Tim Chipman
Looking for antibodies against Cre-recombinase Jennifer Lyon
DNA electrophoresis u.381 at inserm.u-strasbg.fr
lower detection limit with Coomssie? Dima Klenchin
Trizol Susan Boyle-Vavra
NH4Ac-Miniprep becomes bad - why? Michelle Gleeson
Rules of thumb for sequencing cloned PCR products? Bernard Murray
lower detection limit with Coomssie? Bruce W. Ritchings
DNA electrophoresis R Daines
Genetic Analysis Linda St. James
"Biotech Rumor Mill" additions Linda St. James
NH4Ac-Miniprep becomes bad - why? Toumy Guettouche
Hela Cells and Adenylylcylase Components Tracy Blevins
Rules of thumb for sequencing cloned PCR products? Bryan John Maloney
DNA dye for fluorometric quantitation bigcheez at ma.ultranet.com
Affordable Patent Searching breffni at neca.com
postivie control of HIV-1 Amplicor Alex Chang
ssDNA->dsDNA transfos...was:annealing of ssDNA... rhubner at molbiol.ox.ac.uk
Antibodies to cre recombinase Thomas R. Anderson
Perkin-Elmer Taqman System Bharat Patel
lower detection limit with Coomssie? Troels Wind
peptide/antibody production companies Pascal Mertens
Red Cell Band 4.1 Dr. Brian M. Cooke
postivie control of HIV-1 Amplicor Dr. J.P. Clewley
postivie control of HIV-1 Amplicor Dr. J.P. Clewley
ABI 310--help! Agnes Tay
Qiagen: This is ridiculous Oliver S. DePeyer
Remove SDS from protein preparation Chao-Liang Wu
whole mount in situ hybridization a101anne at horus.sara.nl
hemocyte marker? mhlee
cloning in M13 Tony Raizis
Vector for direct cloning of PCR products Dr Ian Graham
NH4Ac-Miniprep becomes bad - why? Keith Leppard
Pipette for PCR Birgit Jostes
Remove SDS from protein preparation Keith Leppard
DNA dye for fluorometric quantitation WSchick at aol.com
Qiagen refuses to tell! Steven Cohen
cloning in M13 Kirk A Bartholomew
Selection with puromycin Danny Altschuler
NH4Ac-Miniprep becomes bad - why? Ralf Spenneberg
Ni-chelation and Mg-binding Troels Wind
blunt end cloning Jin-seon Im
Maxam-Gilbert sequencing ?! Dr Michael Morris
NH4Ac-Miniprep: The Protocol *
Selection with puromycin Ashok Aiyar
enolase Kamran Salim
a good protocol for far westerns/overlays?? Gerald W. Hart
Remove SDS from protein preparation W R BENNETT
NH4Ac-Miniprep: The Protocol Josee Ash
which phophoImager? Randy Nonay
biotinylated nucleotide source? Ed Beaty
which phophoImager? Rork Kuick
more Hybond-N problems Gerald B. Downes
Qiagen refuses to tell! David Micklem
Temperature Sensitive pACYC or Derivatives Michael Benedik
DNA electrophoresis Michael W. Thompson
Qiagen refuses to tell! Michael W. Thompson
Qiagen refuses to tell! Michael W. Thompson
Selection with puromycin Michael W. Thompson
Selection with puromycin Victor Levenson
Qiagen refuses to tell! Michael W. Thompson
NH4Ac-Miniprep: The Protocol Victor Levenson
oligo synthesizer - farewell!!! Norm
Concentrating proteins already in SDS sample buffer? D. Pearton
Selection with puromycin Ashok Aiyar
Qiagen refuses to tell! Richard Daines
Concentrating proteins already in SDS sample buffer? brett
Qiagen: This is ridiculous Steven Cohen
pBEND anyone? gc
DIG Northerns nsaunders at molbiol.ox.ac.uk
Absorbance spectrum for Xgal product Michael B. Elowitz
Yeast contamination on cell culture:HELP!! Kiley R. Prilliman
pBEND anyone? Ashok Aiyar
RNA sizing against DNA markers Dr. Peter Gegenheimer
Selection with puromycin Nikolai Chitaev
a good protocol for far westerns/overlays?? Nikolai Chitaev
ref Quantitative PCR raconte at ibm.net
DNA electrophoresis Maria Bobrowska
Qiagen refuses to tell! Proprietary information. WSchick at aol.com
Disappearing plasmids Arthur Wohlwill

Last message date: Sat Aug 31 15:12:12 EST 1996
Archived on: Sun Mar 6 21:10:22 EST 2005

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