Plant DNA Extraction

Rick Bright rbright at mnw.net
Sun Dec 1 14:27:49 EST 1996


Soren K. Rasmussen wrote:
> 
> In article <329F7510.2CCA at mnw.net>, rbright at mnw.net says...
> >
> >I have successfully extracted DNA from whole blood and frozen blood
> >using a non-toxic (no phenol-chloroform) technique.  It seems to be
> >quite pure without an additional purification step.
> >
> >My next step is to find a simila (non-toxic) technique for Plant DNA.  I
> >can adapt what I have, maybe, changing the detergent and adding an
> >exzyme (papain) step.  I am hoping someone has come across a good
> >method.
> >
> >Also, what type of plant would be best to use initially?  I attempted an
> >onion and it was not that successful.  I would appreciate any ideas for
> >a plant to use that is not as hard to break down.
> >
> >Thanks for your help.
> >
> >Rick Bright
> >
> Dear Rick
> in my experience green fresh leaves are good source for total DNA
> extraction. You may try some from your garden/window but remember best
> results from expanding tissues. Or you may get some seeds, barley, wheat,
> rice or maize and let them germinate for one week. As I am working with
> cereals I will be happy to hear about your non-phenol/chloroform
> procedure.
> soren

Soren --

What is your correct email address?  I receive an error by trying to
reply to this one posted.

Rick



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