What is the typical concentration of a DNA polynucleotide in mols/L after PCR amplification?

Ludvig Mortberg lugmog96 at student.umu.se
Sun Dec 1 10:36:59 EST 1996


If I amplificate a DNA molecule with PCR, approximately what
concentration in mols/L will the solution contain? Yes I know that it
depends on how many cycles I run and how much I started out with in
the beginning. I'm interested in knowing the range people work with
and also the upper limit on how much DNA can be "crowded" in a
solution.

Interested in reading your replies!

Ludvig Mortberg



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