Phenol extraction problem/phases not separating

slavemaster un691cs at genius.embnet.dkfz-heidelberg.de
Mon Dec 2 03:34:41 EST 1996


In article <msnyder.80.329F43AA at ace.acadiau.ca>, msnyder at ace.acadiau.ca 
says...
>
>I am extracting DNA from frog toes collected in lysis buffer 
>:SDS/Tris/EDTA/Sucrose - nothing out of the ordinary.
>
>I am using a standard phenol-chloroform extraction.
>
>Most of my extractions work as expected but about 1 in 8 refuses to separate; 
>the lysis buffer and phenol mix.  

>Ian Paterson

Ian, try adding the salt needed for precipitation before the
phenol/CHCl3 extraction, this may help. Do you really need the
sucrose ?

clemens




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