Phenol extraction problem/phases not separating

Jan Hoolwerf hoolwerf at
Mon Dec 2 01:48:54 EST 1996


msnyder at (MARLENE SNYDER) wrote:

 MS> Most of my extractions work as expected but about 1 in 8 refuses
 MS> to separate; the lysis buffer and phenol mix.

Add a *little* drop of chloroform to the tube.

No garantee but it offen helps me.

with regards, Jan Hoolwerf

******************* these words were mine, all mine !! ********************
 J.D. Hoolwerf
 Neth. Inst. for Dairy Research
 P.O. Box 20                        phone  : 31-318-659511
 6710 BA  Ede                       fax    : 31-318-650400
 the Netherlands                    e-mail : hoolwerf at

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