Phenol extraction problem/phases not separating

[Jan Hoolwerf]

Hello,

msnyder at ace.acadiau.ca (MARLENE SNYDER) wrote:

 MS> Most of my extractions work as expected but about 1 in 8 refuses
 MS> to separate; the lysis buffer and phenol mix.

Add a *little* drop of chloroform to the tube.

No garantee but it offen helps me.


with regards, Jan Hoolwerf

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 J.D. Hoolwerf
 Neth. Inst. for Dairy Research
 P.O. Box 20                        phone  : 31-318-659511
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 the Netherlands                    e-mail : hoolwerf at solair1.inter.nl.net
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