[Q] detecting a mutation site on a BAC clone
Hong- Gil Nam
hgn at WWW.BRIC.POSTECH.AC.KR
Mon Dec 2 21:26:43 EST 1996
I am trying to clone a gene for gi mutataion in Arabidopsis. We have
identified a BAC clone that are very likely to encompass the mutation.
We wish to find where the mutation site is on the BAC clone to get a
clone for funtional complementation by transformation. The BAC clone is
from wild type. We have three independent alleles of mutation, two of
which are by X-ray and gamma-ray mutations.
I would greatly appreciate if someone give an information on this type
of approach to find a mutation on a BAC clone.
Hong Gil Nam
Department of Life Science
Pohang University of Science and Technology
Hyoja DOng, Pohang,
e-mail:hgn at bric.postech.ac.kr
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