RNase removal by phenol-chloroform ?

eric anderson e-anderson at ski.mskcc.org
Mon Dec 2 15:55:31 EST 1996


In article <57utfc$69g at mserv1.dl.ac.uk>, deepak at bgumail.bgu.ac.il (Deepak
Khandka) wrote:

> Is  it possible to remove RNase contamination from a Tris-containing buffer
> by extracting with phenol-chloroform without purturbating the pH of the
> buffer ?

IMO, the easiest way to get RNASE-free Tris is to buy a bottle of Tris
that never gets any scoops or anything put in it (i.e. for RNA use only)
and make the Tris solution in mQ-H2O that has been DEPC treated and
autoclaved to inactivate the DEPC.  we make our Tris and TE for RNA work
this way and have no problems.

good luck,

eric

-- 
Eric C. Anderson
Memorial Sloan-Kettering Cancer Center
Sloan-Kettering Institute
1275 York Ave. Box 470
New York, NY  10028
(212) 639-2977
e-anderson at ski.mskcc.org



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