RNase removal by phenol-chloroform ?

eric anderson e-anderson at ski.mskcc.org
Mon Dec 2 15:55:31 EST 1996

In article <57utfc$69g at mserv1.dl.ac.uk>, deepak at bgumail.bgu.ac.il (Deepak
Khandka) wrote:

> Is  it possible to remove RNase contamination from a Tris-containing buffer
> by extracting with phenol-chloroform without purturbating the pH of the
> buffer ?

IMO, the easiest way to get RNASE-free Tris is to buy a bottle of Tris
that never gets any scoops or anything put in it (i.e. for RNA use only)
and make the Tris solution in mQ-H2O that has been DEPC treated and
autoclaved to inactivate the DEPC.  we make our Tris and TE for RNA work
this way and have no problems.

good luck,


Eric C. Anderson
Memorial Sloan-Kettering Cancer Center
Sloan-Kettering Institute
1275 York Ave. Box 470
New York, NY  10028
(212) 639-2977
e-anderson at ski.mskcc.org

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