RNase removal by phenol-chloroform ?

[Hiranya Roychowdhury]

At 03:43 PM 12/2/96 -0000, Deepak Khandka wrote:
>Is  it possible to remove RNase contamination from a Tris-containing buffer
by extracting with phenol-chloroform

Yes, at least partially. But it is not a foolproof way.


 without purturbating the pH of the
>buffer ?

That depends on the pH of the buffer used originally to equilibrate the phenol.

>
>-------------------------------
>Deepak K Khandka
>Ben-Gurion University
>Israel
>-------------------------------
>
>
>
>
>Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu