RNase removal by phenol-chloroform ?

Hiranya Roychowdhury hroychow at NMSU.EDU
Mon Dec 2 17:36:16 EST 1996

At 03:43 PM 12/2/96 -0000, Deepak Khandka wrote:
>Is  it possible to remove RNase contamination from a Tris-containing buffer
by extracting with phenol-chloroform

Yes, at least partially. But it is not a foolproof way.

 without purturbating the pH of the
>buffer ?

That depends on the pH of the buffer used originally to equilibrate the phenol.

>Deepak K Khandka
>Ben-Gurion University
>Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu

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