Why don't my plasmid freeze in freezer?
Marieke R. Koedood Zhao
rkoedood at bio.bu.edu
Mon Dec 2 12:42:04 EST 1996
We have one freezer where we frequently notice that our plasmid preps
are not frozen. It's a so-called frost-free freezer, i.e. it goes thru
regular defrosting cycles. Since in other freezers our plasmidsa re ALWAYS
forzen, I presume that in this freezer they thaw during the defrosting
Just an idea...
Marieke Koedood Zhao
Takbum Ohn (ahnss at hitel.kol.co.kr) wrote:
: I prepared my plasmid sample for sequencing with Wizard DNA purification
: After washing the column with alcohol(maybe 70% et-OH), I drained plasmid
: attached on resin with hot 300 lambda water and stored in freezer.
: about 24 hours later, i knew that my plasmid sample did't freezed.
: Are there anyone know the cause?
: Tak-bum Ohn.
: ahnss at hitel.kol.co.kr
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