PCR of a 4.9 kb fragment

Christophe beloin at igmors.u-psud.fr
Mon Dec 2 11:44:19 EST 1996

Dear all,
I intend to make an amplification of a 4.9 kb DNA fragment in a plasmid
using a classical Taq polymerase.
Could somebody give me some advice (elongation time, concentration of
MgCl2, ...).
Thanks a lot.

Tophcri. ;-)

             Christophe Beloin
           Beloin at igmors.u-psud.fr
           ;-) ;-) ;-) ;-) ;-) ;-)

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