Rnase removal with phenol:chloroform

Shuiliang Shi sshi at cell-science.org
Mon Dec 2 11:43:14 EST 1996


I would think that treating the tris buffer with phenol:cloroform would
leave a small amount of the organic solvents in the aqueous solution, as
they do have some limited solubility. This may be a problem in downstream
use of the buffer, but who knows?




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