inclusion bodies, purification of

Karin Schott schott at snoopy.org.chemie.tu-muenchen.de
Mon Dec 2 10:16:36 EST 1996


We have an E. coli strain which expresses high levels of a recombinant 
protein. Unfortunately it is made as inclusion bodies and is therfore 
inactive. We would like to purify these inclusion bodies and 
denature/renature the protein. (This has been prooven to yield a 
functional enzyme).
Does anybody know a simple method, (or a literature citation) for 
separating inclusion bodies from bacterial debris? (probably by 
ultracentrifugation?)

Thanks 

Karin Schott



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