plasmid vector with lambdaGEM11-like SfiI sites?

Michael J. Prigge prigge at darkwing.uoregon.edu
Tue Dec 3 02:03:50 EST 1996


Greetings,
Does anyone know of a plasmid with a pair of SfiI sites that match
those of Promega's lambdaGEM11 (aka lambdaEMBO-3 T7/SP6?)?

I am trying to subclone whole inserts from LambdaGEM11 Arabidopsis 
genomic clones. The lambda vector has symmetric EcoRI, BamHI, XhoI, 
and SacI sites flanking the insert; but they all cut in the inserts 
and would require partial digests. There are also the SfiI sites 
flanking the insert. Since the bases overhanging in SfiI sites are 
degenerate, however, I can't clone into any old SfiI site. (The two 
sites in lamGEM11 are different from each other.)

Any help would be appreciated.
Mike
Michael Prigge                    prigge at darkwing.uoregon.edu
Institute of Molecular Biology           University of Oregon
/\\ /\\ /\\ /\\ /\\ /\\ /\\ /\\ /\\ /\\ /\\ /\\ /\\ /\\ /\\ /
/ \\/ \\/ \\/ \\/ \\/ \\/ \\/ \\/ \\/ \\/ \\/ \\/ \\/ \\/ \\/ 



More information about the Methods mailing list