HELP! TBS for GST?

Michael DiDonato michael.didonato at utoronto.ca
Tue Dec 3 14:29:05 EST 1996


I would like to know if anyone has carried out a purification of a GST
fusion protein using Tris-buffered saline instead of PBS as the lysis
buffer?  If so, does the fusion still bind to the column using Tris? At
what pH?

Any suggestions/hints would be greatly appreciated.
Mike

-- 
Michael DiDonato
Department of Biochemistry Research
Hospital for Sick Children
Toronto, Ontario, Canada



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