HELP! TBS for GST?
michael.didonato at utoronto.ca
Tue Dec 3 14:29:05 EST 1996
I would like to know if anyone has carried out a purification of a GST
fusion protein using Tris-buffered saline instead of PBS as the lysis
buffer? If so, does the fusion still bind to the column using Tris? At
Any suggestions/hints would be greatly appreciated.
Department of Biochemistry Research
Hospital for Sick Children
Toronto, Ontario, Canada
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