inclusion bodies (IBs), purification of

slavemaster un691cs at genius.embnet.dkfz-heidelberg.de
Tue Dec 3 08:11:58 EST 1996


In article <5814l9$m6f at mserv1.dl.ac.uk>, wgschech at med.uni-tuebingen.de says...
>
>Clemens, 
>How efffective is this differential centrifugation step (5k/13k)? Do these
>values apply on all kinds of IBs or do I have to experiment?

I arrived at it emperically, so you should do
the same, I guess.
Also, the sonicators or ultrasound producers have
different power level, which could tear your inclusion
bodies to bits. This needs optimization also.

>BTW, there exist other protocols that include differential solubilization
>with increasing amounts of e.g. Urea (2M, 6M).

clemens




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