inclusion bodies (IBs), purification of

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Tue Dec 3 06:58:33 EST 1996


Clemens, 
How efffective is this differential centrifugation step (5k/13k)? Do these
values apply on all kinds of IBs or do I have to experiment?


BTW, there exist other protocols that include differential solubilization
with increasing amounts of e.g. Urea (2M, 6M).

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Wolfgang Schechinger
Chemist            
University of Tuebingen         
Med Clinic IV,
Dpt. of Pathobiochemistry                   
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