inclusion bodies (IBs), purification of

Wolfgang Schechinger wgschech at
Tue Dec 3 06:58:33 EST 1996

How efffective is this differential centrifugation step (5k/13k)? Do these
values apply on all kinds of IBs or do I have to experiment?

BTW, there exist other protocols that include differential solubilization
with increasing amounts of e.g. Urea (2M, 6M).

---------!all possible disclaimers apply!-----------
Wolfgang Schechinger
University of Tuebingen         
Med Clinic IV,
Dpt. of Pathobiochemistry                   
Lab B2/537                      
Otfried-Mueller-Str. 10
72076 Tuebingen, Germany

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